ELAVL1 (NM_001419) Human Untagged Clone

CAT#: SC119271

ELAVL1 (untagged)-Human ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) (ELAVL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELAVL1
• Synonyms: ELAV1; Hua; HUR; MelG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119271 sequence for NM_001419 edited (data generated by NextGen Sequencing)
  ATGTCTAATGGTTATGAAGACCACATGGCCGAAGACTGCAGGGGTGACATCGGGAGAACG
  AATTTGATCGTCAACTACCTCCCTCAGAACATGACCCAGGATGAGTTACGAAGCCTGTTC
  AGCAGCATTGGTGAAGTTGAATCTGCAAAACTTATTCGGGATAAAGTAGCAGGACACAGC
  TTGGGCTATGGCTTTGTGAACTACGTGACCGCGAAGGATGCAGAGAGAGCGATCAACACG
  CTGAACGGCTTGAGGCTCCAGTCAAAAACCATTAAGGTGTCGTATGCTCGCCCGAGCTCA
  GAGGTGATCAAAGACGCCAACTTGTACATCAGCGGGCTCCCGCGGACCATGACCCAGAAG
  GACGTAGAAGACATGTTCTCTCGGTTTGGGCGGATCATCAACTCGCGGGTCCTCGTGGAT
  CAGACTACAGGTTTGTCCAGAGGGGTTGCGTTTATCCGGTTTGACAAACGGTCGGAGGCA
  GAAGAGGCAATTACCAGTTTCAATGGTCATAAACCCCCAGGTTCCTCTGAGCCCATCACA
  GTGAAGTTTGCAGCCAACCCCAACCAGAACAAAAACGTGGCACTCCTCTCGCAGCTGTAC
  CACTCGCCAGCGCGACGGTTCGGAGGCCCCGTTCACCACCAGGCGCAGAGATTCAGGTTC
  TCCCCCATGGGCGTCGATCACATGAGCGGGCTCTCTGGCGTCAACGTGCCAGGAAACGCC
  TCCTCCGGCTGGTGCATTTTCATCTACAACCTGGGGCAGGATGCCGACGAGGGGATCCTC
  TGGCAGATGTTTGGGCCGTTTGGTGCCGTCACCAATGTGAAAGTGATCCGCGACTTCAAC
  ACCAACAAGTGCAAAGGGTTTGGCTTTGTGACCATGACAAACTATGAAGAAGCCGCGATG
  GCCATAGCCAGCCTGAACGGCTACCGCCTGGGGGACAAAATCTTACAGGTTTCCTTCAAA
  ACCAACAAGTCCCACAAATAA
  
  Clone variation with respect to NM_001419.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001419 unedited
  GGTGCAGATTTTGTATACATTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGCCACCG
  CCGCCACCGCTACCGAGGCCGAGCGGAGCCGTTAGCGCCGCGCCGCCGCCGCCTCCCGCC
  CGCCCCGGAGCAGCCCCGGGCCCGCCCGCCCGCATCCAGATTTTTGAAAAATACAATGTC
  TAATGGTTATGAAGACCACATGGCCGAAGACTGCAGGGGTGACATCGGGAGAACGAATTT
  GATCGTCAACTACCTCCCTCAGAACATGACCCAGGATGAGTTACGAAGCCTGTTCAGCAG
  CATTGGTGAAGTTGAATCTGCAAAACTTATTCGGGATAAAGTAGCAGGACACAGCTTGGG
  CTATGGCTTTGTGAACTACGTGACCGCGAAGGATGCAGAGAGAGCGATCAACACGCTGAA
  CGGCTTGAGGCTCCAGTCAAAAACCATTAAGGTGTCGTATGCTCGCCCGAGCTCAGAGGT
  GATCAAAGACGCCAACTTGTACATCAGCGGGCTCCCGCGGACCATGACCCAGAAGGACGT
  AGAAGACATGTTCTCTCGGTTTGGGCGGATCATCAACTCGCGGGTCCTCGTGGATCAGAC
  TACAGGTTTGTCCAGAGGGGTTGCGTTTATTCGGTTTGACAAACGGTCCGAGGCAGAAGA
  GGCCATTACCAGTTTCAATGGTCATAAACCCCCCAGTTCCTTTGAGCCCATTACAGTGAA
  GTTTGCAGCCAACCCCAACCAGTACATAAACGTTGCATTCCTCTTGAAGTTGTCCACTTG
  CCAGGCGACGGTTCTGAGGCCCCGTTCACCACCAGCGCAAAATTTATGTTCTTCCCCATG
  GGCGCTCGTTACATTAGCGGGCTTCTTGGGTTAACGGGCCAGGAAACGCCTCCTCCGGTG
  GTGCATTTTATTCTCAACTGGGGCAGGAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001419 unedited
  TATTCTATGNACCGCGNCCGCATTCTNANGATCGNATTTTTTTTTTTTTTTTTTCGAGCA
  AAACAAAATCAAATTTAATGGTCTTAAATGCAAAAGTAAAAACAAACAAAAAACACAAAA
  AAGCAAAAAAAAATTAACAGAACAGAACAACCCAAAATGTCAAGGCAGTTATGAAGAGAA
  CTTTGAGGGTTAATCTTTAAAACTACAAATCAAAGGTTTTGGTTAATAGATAACTGGCAA
  GAAGTAAGTACTTTTCTGCTGAGTGTTCATACAAAAGCAAAATTCCAAAACAAAATCCGA
  CTAAAATAGAAAAAGGTCTAAGCGCTCACGAGAAGGGATGCGAGAAATACAATGCTCAAA
  TGTTTCTGTGTTATTAAAATACAGAGCCATTTTACGCCTCCAAGCTATGTTAAATGCTCT
  GGAAAACGGGAGAAATTATCGTGAACAACAGTATCCAATGTGTGGGGCGCTGTGCATGCA
  ATGTGTGGACAGAAACTCGGGATGTCAGAGTAGCAACACATCTTCAGGCGTGCCTGGAGC
  TTAGATCTGACTCTGGAATATACTGGAGTTTCCCTGAAACTCTTTGGTCCATTCCCATTG
  GTGCCTGGGCTTACGAAACACGTTTGTGTCCTTTCTGGGAAGGGGCCCGCCCAGCATGCC
  ACCCCGTGGCCCCCGGGCCCCAGTGGTGGGCCCACGCTGGAAGGGCCCCGCCGCCATTCC
  CAGAACCACCTCGGAAAAACCCGGACTGAGTGAACAGGAAGGGGCCAACGGGCCCTGCCT
  GGAAAAGGAACTTTCTGTCAAACAAAGCAAACATGGTTAAAATGTTGAAACGGTTGGAAA
  AAACCACACCCCCCATTTGGTTAAACTCTTTTGTTAAAAACTAAAACTAAAGGGGGTTTT
  TTTGCCTCCCCATCTTGGGGAAAAATGAAAGAAAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001419
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001419.2, NP_001410.2
• RefSeq Size: 6075 bp
• RefSeq ORF: 981 bp
• Locus ID: 1994
• UniProt ID: Q15717
• Cytogenetics: 19p13.2
• Domains: RRM
• Gene Summary: The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]