MR1 (NM_001531) Human Untagged Clone

CAT#: SC119190

MR1 (untagged)-Human major histocompatibility complex, class I-related (MR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MR1
• Synonyms: HLALS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119190 sequence for NM_001531 edited (data generated by NextGen Sequencing)
  ATGGGGGAACTGATGGCGTTCCTGTTACCTCTCATCATTGTGTTAATGGTGAAGCACAGC
  GATTCCCGGACGCACTCTCTGAGATATTTTCGCCTGGGCGTTTCGGATCCCATCCGTGGG
  GTCCCTGAATTTATTTCGGTTGGGTACGTGGACTCGCACCCTATCACCACATATGACAGT
  GTCACTCGGCAGAAGGAGCCACGGGCCCCATGGATGGCAGAGAACCTCGCGCCTGATCAC
  TGGGAGAGGTACACTCAGCTGCTGAGGGGCTGGCAGCAGATGTTCAAGGTGGAACTGAAG
  CGCCTACAGAGGCACTACAATCACTCAGGGTCTCACACTTACCAGAGAATGATTGGCTGT
  GAGCTGCTGGAGGATGGAAGCACCACAGGATTTCTGCAGTATGCATATGACGGGCAGGAT
  TTCCTGATCTTCAATAAAGACACCCTCTCCTGGCTGGCTGTAGATAATGTGGCTCACACC
  ATCAAGCAGGCATGGGAGGCCAATCAGCATGAGTTGCTGTATCAAAAGAATTGGCTGGAA
  GAAGAATGTATTGCCTGGCTAAAGAGATTCCTGGAGTATGGGAAAGACACCCTACAAAGA
  ACAGAGCCCCCACTGGTCAGAGTAAATCGCAAAGAAACTTTTCCAGGGGTTACAGCTCTC
  TTCTGCAAAGCTCATGGCTTTTACCCCCCAGAAATTTACATGACATGGATGAAAAACGGG
  GAAGAAATTGTCCAAGAAATTGATTATGGAGACATTCTTCCCAGTGGGGATGGAACCTAT
  CAGGCGTGGGCATCAATTGAGCTTGATCCTCAGAGCAGCAACCTTTACTCCTGTCATGTG
  GAGCACTGCGGTGTCCACATGGTTCTTCAGGTCCCCCAGGAATCAGAAACTATCCCTCTT
  GTGATGAAAGCTGTCTCTGGGTCCATTGTCCTTGTCATTGTGCTGGCTGGAGTTGGTGTT
  CTAGTCTGGAGAAGAAGGCCCCGAGAGCAAAATGGAGCCATCTACCTTCCAACACCAGAT
  CGATGA
  
  Clone variation with respect to NM_001531.2
  116 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001531 unedited
  TCGATTTGATCGATCCTTAGGCGCCGGATTCGCCGAGCGGAAAGAAAGACTATGGGGGAA
  CTGATGGCGTTCCTGTTACCTCTCATCATTGTGTTAATGGTGAAGCACAGCGATTCCCGG
  ACGCACTCTCTGAGATATTTTCGCCTGGGCGTTTCGGATCCCATCCGTGGGGTCCCTGAA
  TTTATTTCGGTTGGGTACGTGGACTCGCACCCTATCACCACATATGACAGTGTCACTCGG
  CAGAAGGAGCCACGGGCCCCATGGATGGCAGAGAACCTCGCGCCTGATCACTGGGAGAGG
  TACACTCAGCTGCTGAGGGGCTGGCAGCAGATGTTCAAGGTGGAACTGAAGCGCCTACAG
  AGGCACTACAATCACTCAGGGTCTCACACTTACCAGAGAATGATTGGCTGTGAGCTGCTG
  GAGGATGGAAGCACCACAGGATTTCTGCAGTATGCATATGACGGGCAGGATTTCCTGATC
  TTCAATAAAGACACCCTCTCCTGGCTGGCTGTAGATAATGTGGCTCACACCATCAAGCAG
  GCATGGGAGGCCAATCAGCATGAGTTGCTGTATCAAAAGAATTGGCTGGAAGAAGAATGT
  ATTGCCTGGCTAAAGAGATTCCTGGAGTATGGGAAAGACACCCTACAAAGAACAGAGCCC
  CCACTGGTCAGAGTAAATCGCANAGAAACTTTTCCAGGGGTTACAGCTCTCTTCTGCANA
  GCTCATGGCTTTTACCCCCCAGAAATTTACATGACATGGATGANAAACGGNGAAGAAATT
  GTCCAAGAAATTGATTATGGAGACATTCTTCCCCAGTGGGATGGAACCTATCANGCGTGG
  GCATCAATTGAGCTTGATCCTCAGAGCAGCACCCTTACTNCTGTCATGTGGAGCACTGCG
  GTGTCCACATGGGTNCTTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001531 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTCTTTTTTTTTGGAGACGAGTCTTGCTC
  TGTTGCCCAGGCTGGAGTGCAGCGGTGTGATCTCGGCTCACTGCCACCTCTGCCTTATAG
  ATTCAAGTGATTCTCCTGCCTTAGCCTCCCAAGTAGCTGGGACTATAGGCACATGCCACC
  ATGCCCAGCTAATTATTTGTATTTTAGTAGAGACAGGGTTTCACCGTGTTGCCCAGGCTG
  GTCTTGAACTCCTGAGCTCAGGGAATCCGCCTGCCTCAGCCTCCCAAAGTGCTAGGATTA
  CAGGCGTGAGCCACTGTGCCCGGGCTGAAGGTACCTTTATCTGAGGCTGTAATGATTAAA
  GCCAAAGGTCAAATGTCACAGTCAAGGTCAAGGGAGAGCAGAGGTCAAACCTGAGGTGCA
  GGACAAAAGCCACTTGTCAAAGCTCAGGTCACTGAAGTAAGCATTAGGGCTCAGGTGCAG
  AGTCAAACGGGGTCAGAGAAGTACGACCGAGGAATGTGTGACTGGCAGCCCCAGGGCAAG
  TAGCTCCACATGGCAGGGCAGTAGGCGAGGCCTCAGGGGTTGGGGATAGTAAGGAACATT
  AACGGGATGTTCATTCTCACCCATGGGCCTTTTATTGATTCCCAGCGCATATGTATGAAG
  ACAACCTATGTATGAAGGCAAGTTATGCCCAGCTACCATGAGGCAATACAGGAAAGGCAG
  AAATGAGCATTGAGCCCTCTCCTGACACCAAATATCCCGCCCTAATTACTAATATTAATA
  CTATTTTGAGATAAAGCTTCACTCTGTCGCCAGGCTGCAGTGCAGCCGGCGTGATCCCCG
  CCTCACTGCAATCTCTGTCTTCCCGGGTTAAGGCATTCCCCTGGTTCAGCCTTCNAGAGT
  AGTGGGCCCACCCGCACCGCACACCCTTGCCCAGTTTATTTTTTTGTATTTTACAAAGAA
  AGAGGTTCACCTTAATTGGCAGNATAGGTTTTAATTTCCGCAACTTCAGAACCCGCCCGC
  CTCTGTCGCTACAAGAGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001531
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001531.1, NP_001522.1
• RefSeq Size: 1263 bp
• RefSeq ORF: 1026 bp
• Locus ID: 3140
• UniProt ID: Q95460
• Cytogenetics: 1q25.3
• Domains: MHC_I, IGc1
• Protein Families: Transmembrane
• Gene Summary: MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.