ARF1 (NM_001658) Human Untagged Clone

CAT#: SC119130

ARF1 (untagged)-Human ADP-ribosylation factor 1 (ARF1), transcript variant 4


  "NM_001658" in other vectors (5)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ARF1
Synonyms PVNH8
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119130 sequence for NM_001658 edited (data generated by NextGen Sequencing)
ATGGGGAACATCTTCGCCAACCTCTTCAAGGGCCTTTTTGGCAAAAAAGAAATGCGCATC
CTCATGGTGGGCCTGGATGCTGCAGGGAAGACCACGATCCTCTACAAGCTTAAGCTGGGT
GAGATCGTGACCACCATTCCCACCATAGGCTTCAACGTGGAAACCGTGGAGTACAAGAAC
ATCAGCTTCACTGTGTGGGACGTGGGTGGCCAGGACAAGATCCGGCCCCTGTGGCGCCAC
TACTTCCAGAACACACAAGGCCTGATCTTCGTGGTGGACAGCAATGACAGAGAGCGTGTG
AACGAGGCCCGTGAGGAGCTCATGAGGATGCTGGCCGAGGACGAGCTCCGGGATGCTGTC
CTCCTGGTGTTCGCCAACAAGCAGGACCTCCCCAACGCCATGAATGCGGCCGAGATCACA
GACAAGCTGGGGCTGCACTCACTACGCCACAGGAACTGGTACATTCAGGCCACCTGCGCC
ACCAGCGGCGACGGGCTCTATGAAGGACTGGACTGGCTGTCCAATCAGCTCCGGAACCAG
AAGTGA

Clone variation with respect to NM_001658.3
>OriGene 5' read for NM_001658 unedited
ATTTGTATACGACNACTATAGGCGGCCGCGAATTCGGCACGAGGCAACGCCTGGCTNCGG
ACAGCAGCCTCTGAGGTGTCCCTGGCCAGTGTCCTTCCACCTGTCCACAAGCATGGGGAA
CATCTTCGCCAACCTCTTCAAGGGCCTTTTTGGCAAAAAAGAAATGCGCATCCTCATGGT
GGGCCTGGATGCTGCAGGGAAGACCACGATCCTCTACAAGCTTAAGCTGGGTGAGATCGT
GACCACCATTCCCACCATAGGCTTCAACGTGGAAACCGTGGAGTACAAGAACATCAGCTT
CACTGTGTGGGACGTGGGTGGCCAGGACAAGATCCGGCCCCTGTGGCGCCACTACTTCCA
GAACACACAAGGCCTGATCTTCGTGGTGGACAGCAATGACAGAGAGCGTGTGAACGAGGC
CCGTGAGGAGCTCATGAGGATGCTGGCCGAGGACGAGCTCCGGGATGCTGTCCTCCNTGG
TGTTCGCCAACAAGCAGGACCTCCCCAACGCCATGAATGCGGCCGAGATCACAGACAAGC
TGGGGCTGCACTCACTACGCCACAGAACTGGTACATTCANGCCACCTGCGCCACCACGGC
GACGGGCTTATGAGGACTGGACTGGGCTGTCCAATCAGCTCCGGACCCAGAAGTGACGGC
GACCCCNCTCCTTNTCACTCTNNNTGCCTCTGNTTACTCTCATGTGGCAACGTCNGNNTC
GGGGGGGGGAGTGCAAAGCTGCTCCGGGGGTTTTGGCAACCGGGGCATTGACGGGCTGTT
AATTGGGCAGACCAACCTGGCCAGCTTTNAATTATGAAAAAAAAATTTTTGTTCAGAAAG
GGAAGNTTTGGGACCCCTAGCATATATCAACTTTTTTTTTTGAAAGAAATACCCCGGTTG
CTTGCGGAGGAGGGGGGGGCCCCCGGCCCTGCCCAAAAAATTTA
>OriGene 3' read for NM_001658 unedited
TATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
AATAGTTAAGAGATTTTATTCTAATAGCTATAATTACAGTGCTTGTTTGTCGAAATGAAA
ACTGAAAACAAGTATACAAAACAGTTGATTACTAATCGTGTATTGAAAGCAGTAAGAGGT
TCCACGACACCAAATAGACCAGTTCTGAGGTTTCCCCAAGATAAATTTAACAGCTCCAGC
TTCAGTGTTTATCAAAATACAAAAGAAAAAAGTAGAGGTTGTCTTTTTCGATGGCAAATC
GGACCCTTGCAGGCTGAGGGAGAGAAAGCTACATCACACACAGAGGTGGGGTGCTCCCGA
GGGGCTGTGGGTCTAGGTGGACCGCCTGCCCGGCTTGCACGCGCTCCTGCTGGTGAGGCC
CCAGACATCCTGCCAAAGTGTCTGAGCGAGCACGAGTGTTGGGGACGACGGACCCACTCT
GGCCACACGGCGACCGAGGGACAGATGGGGCCCTGCGTCCCATAGGCTGCCTGAAGGTGG
GTAGGGCGGCCTGCGGCATAGTGGGGTGGCTGTGGGCTCCCAGCCTGGCCCCTGGGAACC
GTGGGAGCACAGGGACAAGCACATGGCTATGGAATGCAGGGTGACCCAAGGACAAGCGAG
TTGCGGGGATCTCTACTGTGACCATGCAAAATTGATCGCAGTCTGCTGCGCCACCACCAC
CTCATGTTNCCGAGGGGAACAGCTGGGCTGGCGACTGGCATCCCAGCCCGTACTGCAAAT
TATGCTAGGCCGGGGTCTCCCTTCTGTGTGTTCAGGGTTCTCGACTTGGATTCTTAACTT
TTTAAAAAGCCCCTGAAGTTGGGGTTAAAACCAACCCCCAAATGC
Restriction Sites NotI-NotI     
ACCN NM_001658
Insert Size 1960 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001658.3, NP_001649.1
RefSeq Size 1901 bp
RefSeq ORF 546 bp
Locus ID 375
UniProt ID P84077
Cytogenetics 1q42.13
Domains RAB, SAR, ARF, arf
Protein Pathways Vibrio cholerae infection
Gene Summary ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (4) has an alternate and shorter 5' UTR, as compared to variant 1.

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