ATP6V0C (NM_001694) Human Untagged Clone

CAT#: SC119084

ATP6V0C (untagged)-Human ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c (ATP6V0C), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V0C
• Synonyms: ATP6C; ATP6L; ATPL; VATL; Vma3; VPPC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119084 sequence for NM_001694 edited (data generated by NextGen Sequencing)
  ATGTCCGAGTCCAAGAGCGGCCCCGAGTATGCTTCGTTTTTCGCCGTCATGGGCGCCTCG
  GCCGCCATGGTCTTCAGCGCCCTGGGCGCTGCCTATGGCACAGCCAAGAGCGGTACCGGC
  ATTGCGGCCATGTCTGTCATGCGGCCGGAGCAGATCATGAAGTCCATCATCCCAGTGGTC
  ATGGCTGGCATCATCGCCATCTACGGCCTGGTGGTGGCAGTCCTCATCGCCAACTCCCTG
  AATGACGACATCAGCCTCTACAAGAGCTTCCTCCAGCTGGGCGCCGGCCTGAGCGTGGGC
  CTGAGCGGCCTGGCAGCCGGCTTTGCCATCGGCATCGTGGGGGACGCTGGCGTGCGGGGC
  ACCGCCCAGCAGCCCCGACTATTCGTGGGCATGATCCTGATTCTCATCTTCGCCGAGGTG
  CTCGGCCTCTACGGTCTCATCGTCGCCCTCATCCTCTCCACAAAGTAG
  
  Clone variation with respect to NM_001694.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001694 unedited
  GGGCACTATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCTGGTATT
  TAGAGCGCAGCGGCTGACGGGCCGGATCGCCTTCGCCGCCGCCCGCCCGCAAACCTTCGT
  GCCCGGCCCGTCCTCGCCCCCGCCTCCGCCACCGCCTCGGCCCGCAGAGCTTGCCCCCTC
  CCCACCCGCAGACATGTCCGAGTCCAAGAGCGGCCCCGAGTATGCTTCGTTTTTCGCCGT
  CATGGGCGCCTCGGCCGCCATGGTCTTCAGCGCCCTGGGCGCTGCCTATGGCACAGCCAA
  GAGCGGTACCGGCATTGCGGCCATGTCTGTCATGCGGCCGGAGCAGATCATGAAGTCCAT
  CATCCCAGTGGTCATGGCTGGCATCATCGCCATCTACGGCCTGGTGGTGGCAGTCCTCAT
  CGCCAACTCCCTGAATGACGACATCAGCCTCTACAAGAGCTTCCTCCAGCTGGGCGCCGG
  CCTGAGCGTGGGCCTGAGCGGCCTGGCAGCCGGCTTTGCCATCGGCATCGTGGGGGACGC
  TGGCGTGCGGGGCACCGCCCAGCAGCCCCGACTATTCGTGGGCATGATCCTGATTCTCAT
  CTTCGCCGAAGTGCTCGGCCTCTACGGTCTCATCGTCGACCTCATCCTCTCCACAAAGTA
  GACCCTCTCCGAGCCACCAGCCACAGGATATTATGTAAAGACCACCCCTCCTCATTCCAG
  AACGAACAGNCTGACACATACGCCCGGGGGCCCGCCGCCCCCAAAAGTGGGGCTTGTACC
  ATGCCCATGTCCTAGTGCCCATCGTTTGGTTTTCCCGCCTTGCCCCCGCCCGCCCCCTGC
  CGTGGAAATCTGGGCCCACTAATCGCCCCTTCAAGCCCCCGGGGCCCCCCCCCTAAAAGG
  TTCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001694 unedited
  ATGACCGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTCAACATCCAAAAACTTTATTGGACACAGTTCCGCGCAGGGCCACGGGCCCGGCGGTG
  AGGGCGGTTCCATCCAGAAAGTCTCAGCAACAGGAACCCACAAGGCGACACTCTACCTAA
  GGTTATAAATAGCTGGGAAACAAGGGCCGTTCCGCAAACGCAGGAACAGGCCAAATCTTT
  ATAAATAAACATCCAGTAAAGAAAAATGACAATTCTAAATCATCCGCATACACAAAGCAC
  TCTAGGGGGTGGGGCCCCGGGGGCCTGAAGGGGCAATAAGGGGCCCCAAATGTCCACGGC
  ACGGGGCGGCCGGGGGCAAGGCCGGGGAAACAGACGATGGGCACTAGGACACTGCGCATG
  TACAAGACCAACTACTGGGGGCGGCGGCCCCGTGCGTATGTGTCAGGCTGTTCGTTCTGG
  AATGAGGAGGGGGGGCCTTTACATAATATTCTGAGGCTGGCGGGCTCGGAAAGGTCTACT
  TTGTGGAGAGGATGAGGGCGACAATTACACCGTAAAGGCCGAACACCTCGGGGAAAATGA
  CTTCAGGATCTTGCCCACGAATAATTGGGGTTGTTGGGCGGGGGCCCGTACGCCAAGGGC
  CCCAAAAAGCCTATGGCAAACCCGTCTGCAGGCCCCTTAGCCCCATTTAGGCCGGGCCCA
  ATTGGGGGAACCTTGTAAAGGCTATGCTTAATTTAGGGAATTGCGAAAAGATGGCAACCC
  AAAACGAAAATGGGAAAGAGCCCCCTTCCCATGGGAGAGGGCCTCAAATCCTCCCCCCCC
  CATAAAAACTGTTTAAAGCCCGTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001694
• Insert Size: 1210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001694.2, NP_001685.1
• RefSeq Size: 1126 bp
• RefSeq ORF: 468 bp
• Locus ID: 527
• UniProt ID: P27449
• Cytogenetics: 16p13.3
• Domains: ATP-synt_C
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.