Thymidine Phosphorylase (TYMP) (NM_001953) Human Untagged Clone

CAT#: SC118899

TYMP (untagged)-Human thymidine phosphorylase (TYMP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thymidine Phosphorylase
• Synonyms: ECGF; ECGF1; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; TP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001953, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCACCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCC
  AGGGACTTCCCGACCCTTCGCCAGAGCCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGACGGAGG
  CCGCCTGAGCGAAGCGGACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAG
  ATCGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACCCAGG
  CCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTTGTGGACAAGCATTC
  CACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCCCTGGCGGCATGTGGCTGCAAGGTG
  CCAATGATCAGCGGACGTGGTCTGGGGCACACAGGAGGCACCTTGGATAAGCTGGAGTCTATTCCTGGAT
  TCAATGTCATCCAGAGCCCAGAGCAGATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCA
  GAGTGAGCAGCTGGTTCCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGC
  CTGCCACTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTGGACG
  TTAAGTTCGGAGGGGCCGCCGTCTTCCCCAACCAGGAGCAGGCCCGGGAGCTGGCAAAGACGCTGGTTGG
  CGTGGGAGCCAGCCTAGGGCTTCGGGTCGCGGCAGCGCTGACCGCCATGGACAAGCCCCTGGGTCGCTGC
  GTGGGCCACGCCCTGGAGGTGGAGGAGGCGCTGCTCTGCATGGACGGCGCAGGCCCGCCAGACTTAAGGG
  ACCTGGTCACCACGCTCGGGGGCGCCCTGCTCTGGCTCAGCGGACACGCGGGGACTCAGGCCCAGGGCGC
  TGCCCGGGTGGCCGCGGCGCTGGACGACGGCTCGGCCCTTGGCCGCTTCGAGCGGATGCTGGCGGCGCAG
  GGCGTGGATCCCGGTCTGGCCCGAGCCCTGTGCTCGGGAAGTCCCGCAGAACGCCGGCAGCTGCTGCCTC
  GCGCCCGGGAGCAGGAGGAGCTGCTGGCGCCCGCAGATGGCACCGTGGAGCTGGTCCGGGCGCTGCCGCT
  GGCGCTGGTGCTGCACGAGCTCGGGGCCGGGCGCAGCCGCGCTGGGGAGCCGCTCCGCCTGGGGGTGGGC
  GCAGAGCTGCTGGTCGACGTGGGTCAGAGGCTGCGCCGTGGGACCCCCTGGCTCCGCGTGCACCGGGACG
  GCCCCGCGCTCAGCGGCCCGCAGAGCCGCGCCCTGCAGGAGGCGCTCGTACTCTCCGACCGCGCGCCATT
  CGCCGCCCCCTCGCCCTTCGCAGAGCTCGTTCTGCCGCCGCAGCAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001953 unedited
  GGGNCGGACACGCCAATTCGGCACGAGGGCGAACCCTGAACCCTACGGTCCCGACCCGCG
  GGCGAGGCCGGGTACCTGGGCTGGGATCCGGAGCAAGCGGGCGAGGGCAGCGCCCTAAGC
  AGGCATCCCCGCAGGCCCGGAGCGATGGCAGCCTTGATGACCCCGGGAACCGGGGCCCCA
  CCCGCGCCTGGTGACTTCTCCGGGGAAGGGAGCCAGGGACTTCCCGACCCTTCGCCAGAG
  CCCAAGCAGCTCCCGGAGCTGATCCGCATGAAGCGAGATGGAGGCCGCCTGAGCGAAGCG
  GACATCAGGGGCTTCGTGGCCGCTGTGGTGAATGGGAGCGCGCAGGGCGCACAGATCGGG
  GCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGAGGAGACCTCGGTGCTGACC
  CAGGCCCTGGCTCAGTCGGGACAGCAGCTGGAGTGGCCAGAGGCCTGGCGCCAGCAGCTT
  GTGGACAAGCATTCCACAGGGGGTGTGGGTGACAAGGTCAGCCTGGTCCTCGCACCTGCC
  CTGGCGGCATGTGGCTGCAAGGTGCCAATGATCAGCGGACGTGGTCTGGGGCACACAGGA
  GGCACCTTGGATAAGCTGGAGTCTATTCCTGGATTCAATGTCATCCAGAGCCCAGAGCAG
  ATGCAAGTGCTGCTGGACCAGGCGGGCTGCTGTATCGTGGGTCAGAGTGAGCAGCTGGTT
  CCTGCGGACGGAATCCTATATGCAGCCAGAGATGTGACAGCCACCGTGGACAGCCTGCCA
  CTCATCACAGCCTCCATTCTCAGTAAGAAACTCGTGGAGGGGCTGTCCGCTCTGGTGGTG
  GACGTTAAGTTTCGAAGGGCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001953 unedited
  ATGGCCGCGGCCGCATCTANAGTCGGTTTTTTTTTTTTTTTTTTCGCGGCAAAGGAGCTT
  TATTGCTGCGGCGGCAGAACGAGCTCTGCGAAGGGCGAGGGGGCGGCGAATGGCGCGCGG
  TCGGAGAGTACGAGCGCCTCCTGCAGGGCGCGGCTCTGCGGGCCGCTGAGCGCGGGGCCG
  TCCCGGTGCACGCGGAGCCAGGGGGTCCCACGGCGCAGCCTCTGTCCCACGTCGACCAGC
  AGCTCTGCGCCCACCCCCAGGCGGAGCGGCTCCCCAGCGCGGCTGCGCCCGGCCCCGAGC
  TCGTGCAGCACCAGCGCCAGCGGCAGCGCCCGGACCAGCTCCACGGTGCCATCTGCGGGC
  GCCAGCAGCTCCTCCTGCTCCCGGGCGCGAGGCAGCAGCTGCCGGCGTTCTGCGGGACTT
  CCCGAGCACAGGGCTCGGCCCAGACCGGGATCCACGCCCTGCGCCGCCAGCATCCGCTCG
  AAGCGGCCAAGGGCCCAGCCGTCCTCCAGCGCCGCGGGCACCCGGGCAGCGCCCTGAACC
  TGAGTCCCCGCGGTCCGCTCAGCCAAACCACGCTCCCTCCCACCGTGGCAACCACCCCCC
  TTAAACCTGCGCGGCCCTGCCCCGCCACTCCACCCACACCCCCTCCTTCCACCTCTCGTC
  GCCCGGCCACACACCCCCACCCCTGCGGCTCTCTGTTTCTCGCCGCCCACGACCCCACCT
  TCCCTCGCCCCATCCCCATACCCTCGACCTCCACCCACAACCATCCAGCCCACCTCCTCT
  TCATCCTCCCGTTTTGCCACACTACCTCCAACCCCCACTCCTCCCCTCCACCCCCCATCA
  CACCCCTCCCACCTACTCTATTCCCTCCTCCTCCCCCGATTCCGACCTGCCAGTCTAGAC
  CTCCCCCCCCCTGCCTCCACATTCCCCTCCCTCTCATCAACAAC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001953
• Insert Size: 1660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001953.2, NP_001944.1
• RefSeq Size: 1600 bp
• RefSeq ORF: 1449 bp
• Locus ID: 1890
• UniProt ID: P19971
• Cytogenetics: 22q13.33
• Domains: Glycos_trans_3N
• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• Gene Summary: This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.