FHIT (NM_002012) Human Untagged Clone

CAT#: SC118885

FHIT (untagged)-Human fragile histidine triad gene (FHIT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FHIT
• Synonyms: AP3Aase; FRA3B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118885 sequence for NM_002012 edited (data generated by NextGen Sequencing)
  ATGTCTTTCAGATTTGGCCAACATCTCATCAAGCCCTCTGTAGTGTTTCTCAAAACAGAA
  CTGTCCTTCGCTCTTGTGAATAGGAAACCTGTGGTACCAGGACATGTCCTTGTGTGCCCG
  CTGCGGCCAGTGGAGCGCTTCCATGACCTGCGTCCTGATGAAGTGGCCGATTTGTTTCAG
  ACGACCCAGAGAGTCGGGACAGTGGTGGAAAAACATTTCCATGGGACCTCTCTCACCTTT
  TCCATGCAGGATGGCCCCGAAGCCGGACAGACTGTGAAGCACGTTCACGTCCACGTTCTT
  CCCAGGAAGGCTGGAGACTTTCACAGGAATGACAGCATCTATGAGGAGCTCCAGAAACAT
  GACAAGGAGGACTTTCCTGCCTCTTGGAGATCAGAGGAGGAAATGGCAGCAGAAGCCGCA
  GCTCTGCGGGTCTACTTTCAGTGA
  
  Clone variation with respect to NM_002012.2
  6 g=>t;294 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002012 unedited
  NGGGTTCAAAATTTGNTATACGACTCACTATAGGGCGGCCGCGNAATCGGCACGAGGCTC
  TGTCCGGTCACAGGACTTTTTGCCCTCTGTTCCCGGGTCCCTCAGGCGGCCACCCAGTGG
  GCACACTCCCAGGCGGCGCTCCGGCCCCGCGCTCCCTCCCTCTGCCTTTCATTCCCAGCT
  GTCAACATCCTGGAAGCTTTGAAGCTCAGGAAAGAAGAGAAATCCACTGAGAACAGTCTG
  TAAAGGTCCGTAGTGCTATCTACATCCAGACGGTGGAAGGGAGAGAAAGAGAAAGAAGGT
  ATCCTAGGAATACCTGCCTGCTTAGACCCTCTATAAAAGCTCTGTGCATCCTGCCACTGA
  GGACTCCGAAGAGGTAGCAGTCTTCTGAAAGACTTCAACTGTGAGGACATGTCTTTCAGA
  TTTGGCCAACATCTCATCAAGCCCTCTGTAGTGTTTCTCAAAACAGAACTGTCCTTCGCT
  CTTGTGAATAGGAAACCTGTGGTACCAGGACATGTCCTTGTGTGCCCGCTGCGGCCAGTG
  GAGCGCTTCCATGACCTGCGTCCTGATGAAGTGGCCGATTTGTTTCAGACGACCCAGAGA
  GTCGGGACAGTGGTGGAAAAACATTTCCATGGGACCTCTCTCACCTTTTCCATGCAGGAT
  GGCCCCGAAGCCGGACAGACTGTGAAGCACGTTCACGTCCACGTTCTTCCCAGGAAGGCT
  GGAGACTTTCACAGGAATGACAGCATCTATGAGGAGCTCCAGAAACATGACAGGNAGGAC
  TTTCCTGCCTCTTGGAGATCAGAGGAGGAAATGGCAGCAGAAGCCGCAGCTCTGCGGGTC
  TACTTTCAGTGACACAGGTAAAGACTGCATTTCGTCATTACCCAGAACCCTCCCGTGGCC
  TCAGGAAAGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002012 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TGCAGCAGAAAAGGAAGTTTAATCATAAGGCTGCCGAATAAGGAGACAGGGGGAAACCTC
  AAATCTGCCTGTCTGAGCCGTTTAGGTCTAGGTATTTTAAGGGAGTTGGAGTGACCGAGG
  TGGGGGATCACTGGTTGAAAAATACAGGATGGTGAGATGAAAAAACTGCATTTTCATGCT
  GATTCAGTTCCTCTTGGGGAAAGGCGGGGGGGCGGTCTTCAAACTGGTTGGCAATAGCTC
  TTTTGCTGGAATTCAGGATCTGAAAAACATCTTAAGCCATTTTTAAACAAACAAAGGCCT
  TATGATTCTAACATCAAAAATCTTCTCTGTAGGAACAATGAGGAGGCAAATGGTTAGCAT
  CTGGTGCTCTGGGACTTTTGGTTACCAAGAAGTGGGCCAAAGTGCAGCCTGATTAATGCT
  TAATTACAACTATATTTCTGTCCAGAACCCAGCATCTAATTCTTGTTAACCCTGTGAGGA
  TGGTTTCAATAGAATGTTTCCATTGTCCTTAAAGGGAGGTATAGTTAGTCCCATTATACT
  CCAGGTAATGATGACAGTCTTGGCAAAATCTTAGGACCCTAAGTTGTTTGCCACCTCACA
  ACCTATATTATCCGTCCTAGTCTCAGTTACCANCATCTGACTGCTGGGAAGACTGAGGGT
  GATTTGTTTGGTCTGTGCACAAAACCTTTAAAGAGAATACACATGTTAGTTAACTTAAAA
  ATCCACAGGACTGTGGGCATAGTTTGTGTAACAAGTTAGGGAAATATCTTTCCTGGTAGA
  TGTACAGGGAAGTCATTTACCTGGAAAACAAGAGGGGTACGCCTTCAGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002012
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002012.1, NP_002003.1
• RefSeq Size: 1095 bp
• RefSeq ORF: 444 bp
• Locus ID: 2272
• UniProt ID: P49789
• Cytogenetics: 3p14.2
• Domains: HIT
• Protein Pathways: Non-small cell lung cancer, Purine metabolism, Small cell lung cancer
• Gene Summary: The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1-4 all encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.