c-Jun (JUN) (NM_002228) Human Untagged Clone

CAT#: SC118762

JUN (untagged)-Human jun proto-oncogene (JUN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: c-Jun
• Synonyms: AP-1; AP1; c-Jun; cJUN; p39
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002228 edited
  GAATTCGGCACGAGGGTGGCTGAAGCAGCGAGGCGGGAGTGGAGGTGCGCGGAGTCAGGC
  AGACAGACAGACACAGCCAGCCAGCCAGGTCGGCAGTATAGTCCGAACTGCAAATCTTAT
  TTTCTTTTCACCTTCTCTCTAACTGCCCAGAGCTAGCGCCTGTGGCTCCCGGGCTGGTGT
  TTCGGGAGTGTCCAGAGAGCCTGGTCTCCAGCCGCCCCCGGGAGGAGAGCCCTGCTGCCC
  AGGCGCTGTTGACAGCGGCGGAAAGCAGCGGTACCCACGCGCCCGCCGGGGGAAGTCGGC
  GAGCGGCTGCAGCAGCAAAGAACTTTCCCGGCTGGGAGGACCGGAGACAAGTGGCAGAGT
  CCCGGAGCGAACTTTTGCAAGCCTTTCCTGCGTCTTAGGCTTCTCCACGGCGGTAAAGAC
  CAGAAGGCGGCGGAGAGCCACGCAAGAGAAGAAGGACGTGCGCTCAGCTTCGCTCGCACC
  GGTTGTTGAACTTGGGCGAGCGCGAGCCGCGGCTGCCGGGCGCCCCCTCCCCCTAGCAGC
  GGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCAAGACCCGCCGCCGGCCGGCCACTG
  CAGGGTCCGCACTGATCCGCTCCGCGGGGAGAGCCGCTGCTCTGGGAAGTGAGTTCGCCT
  GCGGACTCCGAGGAACCGCTGCGCCCGAAGAGCGCTCAGTGAGTGACCGCGACTTTTCAA
  AGCCGGGTAGCGCGCGCGAGTCGACAAGTAAGAGTGCGGGAGGCATCTTAATTAACCCTG
  CGCTCCCTGGAGCGAGCTGGTGAGGAGGGCGCAGCGGGGACGACAGCCAGCGGGTGCGTG
  CGCTCTTAGAGAAACTTTCCCTGTCAAAGGCTCCGGGGGGCGCGGGTGTCCCCCGCTTGC
  CAGAGCCCTGTTGCGGCCCCGAAACTTGTGCGCGCAGCCCAAACTAACCTCACGTGAAGT
  GACGGACTGTTCTATGACTGCAAAGATGGAAACGACCTTCTATGACGATGCCCTCAACGC
  CTCGTTCCTCCCGTCCGAGAGCGGACCTTATGGCTACAGTAACCCCAAGATCCTGAAACA
  GAGCATGACCCTGAACCTGGCCGACCCAGTGGGGAGCCTGAAGCCGCACCTCCGCGCCAA
  GAACTCGGACCTCCTCACCTCGCCCGACGTGGGGCTGCTCAAGCTGGCGTCGCCCGAGCT
  GGAGCGCCTGATAATCCAGTCCAGCAACGGGCACATCACCACCACGCCGACCCCCACCCA
  GTTCCTGTGCCCCAAGAACGTGACAGATGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGCG
  CGCCCTGGCCGAACTGCACAGCCAGAACACGCTGCCCAGCGTCACGTCGGCGGCGCAGCC
  GGTCAACGGGGCAGGCATGGTGGCTCCCGCGGTAGCCTCGGTGGCAGGGGGCAGCGGCAG
  CGGCGGCTTCAGCGCCAGCCTGCACAGCGAGCCGCCGGTCTACGCAAACCTCAGCAACTT
  CAACCCAGGCGCGCTGAGCAGCGGCGGCGGGGCGCCCTCCTACGGCGCGGCCGGCCTGGC
  CTTTCCCGCGCAACCCCAGCAGCAGCAGCAGCCGCCGCACCACCTGCCCCAGCAGATGCC
  CGTGCAGCACCCGCGGCTGCAGGCCCTGAAGGAGGAGCCTCAGACAGTGCCCGAGATGCC
  CGGCGAGACACCGCCCCTGTCCCCCATCGACATGGAGTCCCAGGAGCGGATCAAGGCGGA
  GAGGAAGCGCATGAGGAACCGCATCGCTGCCTCCAAGTGCCGAAAAAGGAAGCTGGAGAG
  AATCGCCCGGCTGGAGGAAAAAGTGAAAACCTTGAAAGCTCAGAACTCGGAGCTGGCGTC
  CACGGCCAACATGCTCAGGGAACAGGTGGCACAGCTTAAACAGAAAGTCATGAACCACGT
  TAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGCAAACATTTTGAAGAGAGACCGT
  CGGGGGCTGAGGGGCAACGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002228 unedited
  GAATTCGGCACGAGGGTGGCTGAAGCAGCGAGGCGGGAGTGGAGGTGCGCGGAGTCAGGC
  AGACAGAACAGAACACAGCCAGCCAGCCAGGTCGGCAGTATAGTCCGAACTGCAAATCTT
  ATTTTCTTTTCACCTTCTCTCTAACTGCCCAGAAGCTAGCGCCTGTGGCTCCCGGGCTGG
  TGTTTCGGGAGTGTCCAGAGAGCCTGGTCTCCAGCCGCCCCCGGGAGGAGAGCCCTGCTG
  CCCAGGCGCTGTTGACAGCGGCGGAAAGCAGCGGTACCCACGCGCCCGCCGGGGGAAGTC
  GGCGAGCGGCTGCAGCAGCAAAGAACTTTCCCGGCTGGGAGGACCGGAGACAAGTGGCAG
  AGTCCCGGAGCGAACTTTTGCAAGCCTTTCCTGCGTCTTAGGCTTCTCCACGGCGGTAAA
  GACCAGAAGGCGGCGGAGAGCCACGCAAGAGAAGAAGGACGTGCGCTCAGCTTCGCTCGC
  ACCGGTTGTTGAACTTGGGCGAGCGCGAGCCGCGGCTGCCGGGCGCCCCCTCCCCCTAGC
  AGCGGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCAGACCCNNGCGCGGCCGGCACT
  GCANGGGTCGCACTGATCCGCTCCGCGGGGAGAGCCGCTGCTTGGGAAGTGAGGTCGCCT
  GCGGGACTCGAGAACCGCTGCGCCGAGAGCGCTCAGTGAGTGACCGCGACTTTTCAAGCC
  GGNTANCGCGCGCGAGTCCACAGTTAAAAGTGCGGGAGGCATCTTATTTAACCCTGCGCT
  CCCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002228 unedited
  TTAGCTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCGTTGC
  CCCTCAGCCCCCGACGGTCTCTCTTCAAAATGTTTGCAACTGCTGCGTTAGCATGAGTTG
  GCACCCACTGTTAACGTGGTTCATGACTTTCTGTTTAAGCTGTGCCACCTGTTCCCTGAG
  CATGTTGGCCGTGGACGCCAGCTCCGAGTTCTGAGCTTTCAAGGTTTTCACTTTTTCCTC
  CAGCCGGGCGATTCTCTCCAGCTTCCTTTTTCGGCACTTGGAGGCAGCGATGCGGTTCCT
  CATGCGCTTCCTCTCCGCCTTGATCCGCTCCTGGGACTCCATGTCGATGGGGGACAGGGG
  CGGTGTCTCGCCGGGCATCTCGGGCACTGTCTGAGGCTCCTCCTTCAGGGCCTGCAGCCG
  CGGGTGCTGCACGGGCATCTGCTGGGGCAGGTGGTGCGGCGGCTGCTGCTGCTGCTGGGG
  TTGCGCGGGAAAGGCCAGGCCGGCCGCGCCGTAGGAGGGCGCCCCGCCGCCGCTGCTCAN
  CGCGCCTGGGTTGAAGTTGCTGAGGTTTGCGTAAACCGGCGGCTCGCTGTGCAGGCTGGC
  GCTGAANCCGCCGCTGCCGCTGCCCCCCTGCCACCGAGGCTACCGCGGGAGCCACCATGC
  CTGCCCCGTTGACCGGCTGCGCCGCCGACGTGACGCTGGGCAGCGTGTTCTGGCTGTGCC
  AATTCGCCAGGGCGCGCACGAAACCCTTCGCGAAGCCCTCCTGCTCATCTGTCACGTTCT
  TGGGGCACAGGAACTGGGGTGGGGGTCGCGGTGGGGGGGAATTACCCCCTTCTTGGACTG
  GATTATAAGGGCCTTCACTTGGGGGGAGGCCAGCTTGAGAATCCCCACCTCGGGGAGGTG
  GGGGGGGCTCATTTCTTGGTCCCGGGGGGCGGGTTCAAGCTCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002228
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002228.3, NP_002219.1
• RefSeq Size: 3338 bp
• RefSeq ORF: 996 bp
• Locus ID: 3725
• UniProt ID: P05412
• Cytogenetics: 1p32.1
• Domains: BRLZ, Jun
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Renal cell carcinoma, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway
• Gene Summary: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]