Integrin beta 8 (ITGB8) (NM_002214) Human Untagged Clone

CAT#: SC118752

ITGB8 (untagged)-Human integrin, beta 8 (ITGB8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Integrin beta 8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002214, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGGCGAGGTCCCG
  CCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGCCAAGGTGAAGACAATAGATG
  TGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCGCTGGGTCCAGAATGTGGATGGTGTGTTCAA
  GAGGATTTCATTTCAGGTGGATCAAGAAGTGAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCT
  GCTCAGTTGATTCAATAGAATACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCA
  GGTGACACCAGGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
  CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCACAATAATATAG
  AAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTCTCCCGTGACTTTCGTCTTGG
  ATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATTAGCATCCACCCCGAAAGGATTCATAATCAA
  TGCAGTGACTACAATTTAGACTGCATGCCTCCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACA
  TCACTGAGTTTGAGAAAGCAGTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTT
  TGACGCCATGCTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
  CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTGGTGCCCAATG
  ACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATGGAACACCCCTCACTAGGCCA
  ACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATCTTTGCAGTTCAAGGAAAACAATTTCATTGG
  TATAAGGATCTTCTACCCCTCTTGCCAGGCACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCA
  ATAATTTGGTAGTGGAAGCCTATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACA
  AGGCATCTATTTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
  AACGTGACGAGCAATGATGAAGTTCTTTTCAATGTAACAGTTACAATGAAAAAATGTGATGTCACAGGAG
  GAAAAAACTATGCAATAATCAAACCTATTGGTTTTAATGAAACCGCTAAAATTCATATACACAGAAACTG
  CAGCTGTCAGTGTGAGGACAACAGAGGACCTAAAGGAAAGTGTGTAGATGAAACTTTTCTAGATTCCAAG
  TGTTTCCAGTGTGATGAGAATAAATGTCATTTTGATGAAGATCAGTTTTCTTCTGAGAGTTGCAAGTCAC
  ACAAGGATCAGCCTGTTTGCAGTGGTCGAGGAGTTTGTGTTTGTGGGAAATGTTCATGTCACAAAATTAA
  GCTTGGAAAAGTGTATGGAAAATACTGTGAAAAGGATGACTTTTCTTGTCCATATCACCATGGAAATCTG
  TGTGCTGGGCATGGAGAGTGTGAAGCAGGCAGATGCCAATGCTTCAGTGGCTGGGAAGGTGATCGATGCC
  AGTGCCCTTCAGCAGCAGCCCAGCACTGTGTCAATTCAAAGGGCCAAGTGTGCAGTGGAAGAGGCACGTG
  TGTGTGTGGAAGGTGTGAGTGCACCGATCCCAGGAGCATCGGCCGCTTCTGTGAACACTGCCCCACCTGT
  TATACAGCCTGCAAGGAAAACTGGAATTGTATGCAATGCCTTCACCCTCACAATTTGTCTCAGGCTATAC
  TTGATCAGTGCAAAACCTCATGTGCTCTCATGGAACAACAGCATTATGTCGACCAAACTTCAGAATGTTT
  CTCCAGCCCAAGCTACTTGAGAATATTTTTCATCATTTTCATAGTTACATTCTTGATTGGGTTGCTTAAA
  GTCCTGATCATTAGACAGGTGATACTACAATGGAATAGTAATAAAATTAAGTCCTCATCAGATTACAGAG
  TGTCAGCCTCAAAAAAGGATAAGTTGATTCTGCAAAGTGTTTGCACAAGAGCAGTCACCTACCGACGTGA
  GAAGCCTGAAGAAATAAAAATGGATATCAGCAAATTAAATGCTCATGAAACTTTCAGGTGCAACTTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002214 unedited
  GGGCGGCCCGCGAATTCGCACGAGGGGACTGCGGGACGCCTGAGCCGCGCACTGAGGCGA
  AAAGGACAAGGGCACGCAGCCCCCGCCCCGCGAAGCCGGGCTCCGGCACCTCGCGGAACC
  TCCCCTCCTGCGCTCGCAGCCGCAGGCCCCACTCCCCGCCAGGGAGGGAGCGGCGATGCC
  TCGCTCTGTGCCTGCGGGTGTCGGCGGGTGCTTCTAGGGCGCTCCCAGAGCCGCCTCCCC
  CTGTTGCTGGCATCCCGAGCTTCCTCCCTTGCCAGCCAGGACGCTGCCGACTTGTCTTTG
  CCCGCTGCTCCGCAGACGGGGCTGCAAAGCTGCAACTAATGGTGTTGGCCTCCCTGCCCA
  CCTGTGGAAGCAACTGCGCTGATTGATGCGCCACAGACTTTTTTCCCCTCGACCTCGCCG
  GCGTCCCCTCCCACAGATCCAGCATCACCCAGTGAATGTACATTAGGGTGGTTTCCCCCC
  CAGCTTCGGGCTTTGTTTGGGTTTGATTGTGTTTGGCTCTTCGCTAAGCTGATTTATGCA
  GCAGAAGCCCCACCGGCTGGAGAGAAACAAAAGCTCTTTTCTTTGTCCAGGAGCANGCTG
  CGGAGCCCTTGCAGAGCCCTCTCTCCAGTCGCCGCCGGAGCCCTTGGCCGTCGAAGGAAG
  TGCTTCTCGCGGAGACCGCGGGACCCGCCGTGCCGAGCCGGGAGGGCCGCAGGGGCCCTG
  AGATGCCGAGCGGTGCCCGGGCCCGCTTACCTGCACCGCTTGCTCCCGAGCCGGGGGTCC
  GNCTGCTNAGCCTGCNGAAAACGTCCTANCGACACTCGGNCCGCGGGCCCCGAGGTGCGC
  CGCGGAGGCGCGAGCCCCGGTCCGGAAGGAANCAGCCGGCGGCCGCGGGCGGGCTGTTTG
  CATTAGTGCGCCTCGCCCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002214 unedited
  GTTTACTTGNACCCGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGAAAGAT
  GAAACACGAATCATTTATTCATCTAGCTGTTCTTGTCTATTCAACCACCAACTGTTGAAG
  TGTTTGGCAACCTTAGAATATACTTGCCAGATTATTTTTTTCCTCACATGTCCAGTGCAG
  GGATTACTGTACCCTTGTAGTGACACAATGCTAAACTCTCTCACAGCATTGTTCTCTCTG
  GGAAGCGTAGGGATAACAGGTACCTGTAAAGTGTAATCAGTGCTCTTCAGAGGATCTACA
  TTCTATGCTGATCTAAATAAGTTATATATTACAGTAAAGTGCTACAACGACACTAGTCTC
  AAACAGTAGTAAGACACATTTTTCTCTGAAAAAGTCAGCAGCTATGTGAGTCACATCATG
  ATGAGGATACTTGTCAGTCTTCGTTCGAGTGTACAACCAGCAACTGGCATGACCGTGAGC
  AATTTGCCTCCTCCTGTGACTTTTAAAATTATAATCTTTAGGAGCAATTATTAACAATTC
  CAGTCTTCCCATTAAGTGTTTAAAAATCCTTTTTTACAAGTCGCACCTGAAAGCCCATGA
  GCCTTCAATCCCGCTGCTATCCATTCTTATTTCCTCAGGCCTCTCACGTCCGTAGGCGAT
  CGCTCTTGGGCCAACACTTTGCAGAATCAACCTATCCTTTTTTGAGGCTGCCCCTCTGCC
  ATCCCCGCAGGCCCCAACGCCCCCAGCACCCCCCTCGCAATCTCCACCTGCCCAATGATC
  CAGGATTTTCACTCATCCCCATTTCCAATGCTCCCCTTCTACTGATGAAATACCTCCCCC
  GGTAGCTTGTCTTGCACCACAATTCCTTAGCCCTGTCATCTTATGCCTCTCTCCCCTTAC
  ACCACCCACGCCCCACCCTCAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002214
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002214.1, NP_002205.1
• RefSeq Size: 8787 bp
• RefSeq ORF: 8787 bp
• Locus ID: 3696
• UniProt ID: P26012
• Cytogenetics: 7p21.1
• Domains: INB, PSI
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• Gene Summary: This gene is a member of the integrin beta chain family and encodes a single-pass type I membrane protein with a VWFA domain and four cysteine-rich repeats. This protein noncovalently binds to an alpha subunit to form a heterodimeric integrin complex. In general, integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]