NDUFA4 (NM_002489) Human Untagged Clone

CAT#: SC118617

NDUFA4 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa (NDUFA4), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFA4
• Synonyms: CI-9k; CI-MLRQ; COXFA4; MC4DN21; MISTR1; MLRQ; MRCAF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118617 sequence for NM_002489 edited (data generated by NextGen Sequencing)
  ATGCTCCGCCAGATCATCGGTCAGGCCAAGAAGCATCCGAGCTTGATCCCCCTCTTTGTA
  TTTATTGGAACTGGAGCTACTGGAGCAACACTGTATCTCTTGCGTCTGGCATTGTTCAAT
  CCAGATGTTTGTTGGGACAGAAATAACCCAGAGCCCTGGAACAAACTGGGTCCCAATGAT
  CAATACAAGTTCTACTCAGTGAATGTGGATTACAGCAAGCTGAAGAAGGAACGTCCAGAT
  TTCTAA
  
  Clone variation with respect to NM_002489.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002489 unedited
  CACGAGGTGACTTTGGAAGTCCGTAGTGTCTCATTGCAGATAATTTTTAGCTTAGGGCCT
  GGTGGCTAGGTCGGTTCTCTCCTTTCCAGTTGGAGACCTCTGCCGCAAACATGCTCCGCC
  AGATCATCGGTCAGGCCAAGAAGCATCCGAGCTTGATCCCCCTCTTTGTATTTATTGGAA
  CTGGAGCTACTGGAGCAACACTGTATCTCTTGCGTCTGGCATTGTTCAATCCAGATGTTT
  GTTGGGACAGAAATAACCCAGAGCCCTGGAACAAACTGGGTCCCAATGATCAATACAAGT
  TCTACTCAGTGAATGTGGATTACAGCAAGCTGAAGAAGGAACGTCCAGATTTCTAAATGA
  AATGTTTCACTATAACGCTGCTTTAGAATGAAGGTCTTCCAGAAGCCACATCCGCACAAT
  TTTCCACTTAACCAGGAAATATTTCTCCTCTAAATGCATGAAATCATGTTGGAGATCTCT
  ATTGTAATCTCTATTGGAGATTACAATGATTAAATCAATAAATAACTGAAACTTGAAAAA
  AAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGAT
  CCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACT
  CCAGTGCCCACCAGCCTTGTCCTAATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGT
  CCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002489 unedited
  ATCCTGCACCGCGCCGCATTTTANGATCGGTTTTTTTTTTTTTTTTTTCAAGTTTCAGTT
  ATTTATTGATTTAATCATTGTAATCTCCAATAGAGATTACAATAGAGATCTCCAACATGA
  TTTCATGCATTTACAGGAGAAATATTCCCTGGTTAAGTGGAAAATTGTGCGGATGTGGCT
  TCTGGAAGACCTTCATTCTAAAGCAGCGTTATAGTGAAACATTTCATTTACAAATCTGGA
  CGTTCCTTCTTCAGCTTGCTGTAATCCACATTCACTGAGTAAAACTTGTATTGATCATTG
  GGACCCAGTTTGTTCCAGGGCTCTGGGTTATTTCTGTCCCAACAAACATCTGGATTGAAC
  AATGCCAGACGCAAGAGATACAGTGTTGCTCCAGTACCTCCAGTTCCAATAAATCCAAAG
  AGGGGGCTCAAGCTCGGATGCTTCTTGGCCTGACCGCAGATCTGGCGGAGCATGCTTGCG
  GCACAGGTCTCCAACTGGAAAGGAGAGAACCGACCTACCCCCAGGCCCTAAGCTAAAAAT
  TATCTGCAATGAGACACTACGGACTTCCAAAGTCACCTCGTGCCGAATTCCCGGCCGCCC
  TATAGGGAGTCCGATTCCAAAATTCTGACGGTTCCCCTAACGAGCTCCTGTTATATAGAC
  CTCCCACCGGACACGCCTACCGCCCATTTGCGCAACCGGGCCGGGGTTATCACCACATTT
  TTGAAAGTCCCCTTGAATTTGGTGCCCAAACAAACTCCCTTGGACCCCATGGGGCGGACC
  CTGGCAAACCCCCGCAGTAAACCCTTTCCCCCCCCATTGGGTCCCGCAAAACCCACTCCC
  AGGGCAAAACCAGTCCTACACCGCAAACGCCCTGCCACCCGGAAAACCCCCCAAGGTCCT
  CCCTCTGCCCCAATCCAAGCGGGCCCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002489
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002489.2, NP_002480.1
• RefSeq Size: 823 bp
• RefSeq ORF: 246 bp
• Locus ID: 4697
• UniProt ID: O00483
• Cytogenetics: 7p21.3
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]