PKC beta 1 (PRKCB) (NM_002738) Human Untagged Clone

CAT#: SC118454

PRKCB (untagged)-Human protein kinase C, beta (PRKCB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKC beta 1
• Synonyms: PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1; PRKCB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002738 edited
  ATGGCTGACCCGGCTGCGGGGCCGCCGCCGAGCGAGGGCGAGGAGAGCACCGTGCGCTTC
  GCCCGCAAAGGCGCCCTCCGGCAGAAGAACGTGCATGAGGTCAAGAACCACAAATTCACC
  GCCCGCTTCTTCAAGCAGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGCTTC
  GGGAAGCAGGGATTCCAGTGCCAAGTTTGCTGCTTTGTGGTGCACAAGCGGTGCCATGAA
  TTTGTCACATTCTCCTGCCCTGGCGCTGACAAGGGTCCAGCCTCCGATGACCCCCGCAGC
  AAACACAAGTTTAAGATCCACACGTACTCCAGCCCCACGTTTTGTGACCACTGTGGGTCA
  CTGCTGTATGGACTCATCCACCAGGGGATGAAATGTGACACCTGCATGATGAATGTGCAC
  AAGCGCTGCGTGATGAATGTTCCCAGCCTGTGTGGCACGGACCACACGGAGCGCCGCGGC
  CGCATCTACATCCAGGCCCACATCGACAGGGACGTCCTCATTGTCCTCGTAAGAGATGCT
  AAAAACCTTGTACCTATGGACCCCAATGGCCTGTCAGATCCCTACGTAAAACTGAAACTG
  ATTCCCGATCCCAAAAGTGAGAGCAAACAGAAGACCAAAACCATCAAATGCTCCCTCAAC
  CCTGAGTGGAATGAGACATTTAGATTTCAGCTGAAAGAATCGGACAAAGACAGAAGACTG
  TCAGTAGAGATTTGGGATTGGGATTTGACCAGCAGGAATGACTTCATGGGATCTTTGTCC
  TTTGGGATTTCTGAACTTCAGAAAGCCAGTGTTGATGGCTGGTTTAAGTTACTGAGCCAG
  GAGGAAGGCGAGTACTTCAATGTGCCTGTGCCACCAGAAGGAAGTGAGGCCAATGAAGAA
  CTGCGGCAGAAATTTGAGAGGGCCAAGATCAGTCAGGGAACCAAGGTCCCGGAAGAAAAG
  ACGACCAACACTGTCTCCAAATTTGACAACAATGGCAACAGAGACCGGATGAAACTGACC
  GATTTTAACTTCCTAATGGTGCTGGGGAAAGGCAGCTTTGGCAAGGTCATGCTTTCAGAA
  CGAAAAGGCACAGATGAGCTCTATGCTGTGAAGATCCTGAAGAAGGACGTTGTGATCCAA
  GATGATGACGTGGAGTGCACTATGGTGGAGAAGCGGGTGTTGGCCCTGCCCGGGAAGCCG
  CCCTTCCTGACCCAGCTCCACTCCTGCTTCCAGACCATGGACCGCCTGTACTTTGTGATG
  GAGTACGTGAATGGGGGCGACCTCATGTATCACATCCAGCAAGTCGGCCGGTTCAAGGAG
  CCCCATGCTGTATTTTACGCTGCAGAAATTGCCATCGGTCTGTTCTTCTTACAGAGTAAG
  GGCATCATTTACCGTGACCTAAAACTTGACAACGTGATGCTCGATTCTGAGGGACACATC
  AAGATTGCCGATTTTGGCATGTGTAAGGAAAACATCTGGGATGGGGTGACAACCAAGACA
  TTCTGTGGCACTCCAGACTACATCGCCCCCGAGATAATTGCTTATCAGCCCTATGGGAAG
  TCCGTGGATTGGTGGGCATTTGGAGTCCTGCTGTATGAAATGTTGGCTGGGCAGGCACCC
  TTTGAAGGGGAGGATGAAGATGAACTCTTCCAATCCATCATGGAACACAACGTAGCCTAT
  CCCAAGTCTATGTCCAAGGAAGCTGTGGCCATCTGCAAAGGGCTGATGACCAAACACCCA
  GGCAAACGTCTGGGTTGTGGACCTGAAGGCGAACGTGATATCAAAGAGCATGCATTTTTC
  CGGTATATTGATTGGGAGAAACTTGAACGCAAAGAGATCCAGCCCCCTTATAAGCCAAAA
  GCTTGTGGGCGAAATGCTGAAAACTTCGACCGATTTTTCACCCGCCATCCACCAGTCCTA
  ACACCTCCCGACCAGGAAGTCATCAGGAATATTGACCAATCAGAATTCGAAGGATTTTCC
  TTTGTTAACTCTGAATTTTTAAAACCCGAAGTCAAGAGCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002738 unedited
  AGCCTNTCGCACGAGCACCTCTCGGTCTCCGGCTCCCCGCGCGCAAAAGGCTGACCCGGC
  TGCGGGGCCACCGCCGAGCGAGGGCGAGGAGAGCACCAGGCGCTTCGCCCGCAAAGGCGC
  CCTCCGGCAGAAGAACGTGCATGAGGTCAAGAACCACAAATTCACCGCCCGCTTCGCTAC
  AGCCCACCTTCTGCAGCCTCTGCACCGACTTTATCTGGGGCTTCGGGAAGCAGGGATTCC
  AGTGCCAAGTTTGCTGCTTTGTGGTGCACAAGCGGTGCCATGAATTTGTCACATTCTCCT
  GCCCTGGCGCTGACAAGGGTCCAGCCTCCGATGACCCCCGCAGCAAACACAAGTTTAAGA
  TCCACACGTACTCCAGCCCCACGTTTTGTGACCACTGTGGGTCACTGCTGTATGGACTCA
  TCCACCAGGGGATGAAATGTGACACCTGCATGATGAATGTGCACAAGCGCTGCGTGATGA
  ATGTTCCCAGCCTGTGTGGCACGGACCACACGGAGCGCCGCGGCCGCATCTACATCCAGG
  CCCACATCGACAGGGACGTCCTCATTGTCCTCGGTAAGAGATGCTAAAAACCTTGTACCT
  ATGGACCCCAATGGCCTGTCAAATCCCTACGTTAAACTGAAACTGATTCCCGATCCCAAA
  GTGAGAGCAAACGGAAAACCCAAACCATCAAATGCTCCCTCAACCCTGAGTGGAATGAGA
  CCATTAGATTTCAGCTGAAAAAATCGAACAAGACAGAAGACTGGCATTAGAGATTTGGGA
  TTGGGAATTTGCCCGCCGGAAAGACCTTCCTGGGATCCTTGGCCTTTGGGAATTTTTAAA
  CTTAAAAAAGCCATTGTGGATGGCTGGCTTAAGTTACCTGACCCGGGAGAATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002738 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTTTTTTTTTGCATTTGAA
  CAACAACAGAAACAAAACCCCAAAGCAAATAAAGTTTGAAATGTAATGTAAAACCAGGGC
  CCTTTGGGGGCCTTGCTAGAGTGACTGTGTTGTTTCAGAGAGTGGTGCCAAATGCCCTTT
  TCAAGTAGTTTGGGGATGAGGGTGAGCAAAGACATAAAGTATCAGAAACTACAACACTGA
  GGTAGCACCCAAGCTGGTTGCCCTTTGACCCCTTGAAGTGCTTTTAGATTGAGACCGTCT
  AAAAGCACATTCTTGGAATTTTAGAACTTTGATCATCTTAACTTTGAGTTTACAAAAATA
  ACAGATAACATTTTATTTTGAGCATGTTCTTTTCAAGGTCTGGTGCCTCCAGAACAGGTG
  AAATAGAGGGATTCCTCTTAGGCAAAGCAACCATACACTTGGATACAAGCCAAGCTCCAG
  GCCCGGAAGTGGAAGAGTACCGCTAACATCCAATTATCCACAGATTGGATGGTTGGGGGT
  GAATATGGCAGTGCAGAAAAAGAGGGAAAGAAAGCAGGCATTTTCTCTCCCCATTGGGTC
  TACTTAGCTGACATGCTGCGGAAAATAAATTGTAAGAGGACTTTGATAATGTGTTAATTA
  TGCAGCTGCATTTTCCATAGGCCATCTGCATAATCCCATCTCATAGAGATGCTCCAAACT
  ACCTGAGGACATTTTGGNNTCTGAACACTGTCACTCTAGTCTCATCAGAAAATCAAACAT
  GGNATGCACTTGNNCATGAAAACATAAAATGTCCCACAATAGCCGTTGAGCTTGATGACA
  GAATGAAGGACGGAGACTACCATCTACTTAGCTTTGACTTCGGTTTTAAAAATCAGAGTA
  CAAGAAATCCTTCGATCTGATGGNCATATTCTGAGACTTCTGGNCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002738
• Insert Size: 2900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002738.5, NP_002729.2
• RefSeq Size: 3411 bp
• RefSeq ORF: 2022 bp
• Locus ID: 5579
• UniProt ID: P05771
• Cytogenetics: 16p12.2-p12.1
• Domains: C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Calcium signaling pathway, Chemokine signaling pathway, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.