RFC4 (NM_002916) Human Untagged Clone

CAT#: SC118346

RFC4 (untagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC4
• Synonyms: A1; RFC37
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118346 sequence for NM_002916 edited (data generated by NextGen Sequencing)
  ATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGA
  GGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGG
  GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA
  GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT
  GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT
  TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA
  GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG
  TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG
  GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT
  AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG
  CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC
  AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA
  GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA
  GTGATTACAGACATTGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC
  TGTCAGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGT
  CATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCT
  GATAAACAGAAGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGAT
  GGTGCTGATGAACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCT
  CAGAATTGTTAA
  
  Clone variation with respect to NM_002916.3
  798 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002916 unedited
  TTTGTATACGACTCACTATGGGCGGCNCGCGAATTCGCACGAGGGACTGTGGGGACATCA
  GTGATCGTAAGTCTCCTGGGCCCGTTATTCTCAGATTAGGTGACGGAGCTAAGACTTCGA
  GACCATCTCGTCCTTTTTGTATCGCGGAAACCTGAGGAACGAGCCGGCGGCGGTGACCTG
  CACGAGAAGCCAGGCTAACTGGGTGAAGTACCATGCAAGCATTTCTTAAAGGTACATCCA
  TCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCG
  GAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGTGTGG
  ATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATCTTTAGAAGGAGCAG
  ATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAAAAACATCCACTATTTTGG
  CAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAAGAGTTCTTGAGTTAAATG
  CATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAA
  CTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATTCTGG
  ATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACGTACCATGGAGAAGG
  AGTCGAAAACCACCCCGATCTGTCTTATCTGTAACTATGTCAGTCGAATAATTGAACCCC
  TGACCTCTAGATGTTCAAAATTCCGCCTTCAGGCTCTGTCAGATAAATTCAACAGCAGCG
  ATTACTAGACATTGCCCAGAAGGAAATGTCAAAATAGGATGAGGGATAGCTATCTTGGTT
  AGGGTCGAGAGACTTAGAAAGCCTTCATTCTTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002916 unedited
  TTTGGACGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTGGGCATTTTATTTTTATTACAACTTCATTATTTACAAAACCCCCCATCCAGA
  TATATTCACGTTAACAATTCTGAGATAACTGCTGCATCACAGTTGCACAAAGGCTGATGA
  GTTGCAAATGTTCATCAGCACCATCTGCTAGGCATTTGTCAACTTCCTACCAGAAAAATT
  TAAGAAATTAGCATCCTTCAGGTAGTTAAATGTTCCATTGACATGTGCAAAGTACCTACT
  TACGGCAAGTTTTTCTGGGATAATAAACTTCTGTTTATCAAATAAGTTATTTTCTACAAC
  CACATCATGGAGTTGATTGACCAGCTGAGTTGCTGCATGACCCTCATCTATTAAATCCTA
  TAATAAAAAAAACTTTTGGTATGATGACTTAATATTCCTTTCCCCAAAGTTAGTAAGCTG
  ACTTTACCTTGACCACAGCTTCTAATTTGTCAAAAGAGCCACTCTGACAGGCAGCAAATA
  CTCCATCAATTTTCTCAGCTGGTATTACCTAAGTAATTGAATGTTCGGTATTAAAGATGT
  TTCTAGGTGAGACTAGACATACACTCTGCCAACTGGCCCCCGTGCCCCCAGTCATTTCTG
  TTCCACACCTAAGTTCCATACTTTTCTGGGAAGGCTTTGGGAGAACGGAAGGGCCAGAGT
  TACTCCAGCCACCACTAAGCAACCAAGTGCTAACAACTGTGCTTTTAAAGCCCTTTTATG
  GCCTTGTGAACTTCCAAGTTTGGGCACACATGGATTATAGCCTGNTCTGGTCAGGGGATG
  AAGATTAAGTTTTCCCCCTAGCGCTTTACGATTCCTCAAACAGTGGCCAGATCACCACAA
  GACTGATTNCTTAAAGGGNAGTGCCTTCTCTCATCTCTCCTGTAAGATGCACTATGAGAT
  GGNTTAAAGTCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002916
• Insert Size: 2580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002916.3, NP_002907.1
• RefSeq Size: 1427 bp
• RefSeq ORF: 1092 bp
• Locus ID: 5984
• UniProt ID: P35249
• Cytogenetics: 3q27.3
• Domains: AAA, AAA
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.