STAT6 (NM_003153) Human Untagged Clone

CAT#: SC118195

STAT6 (untagged)-Human signal transducer and activator of transcription 6, interleukin-4 induced (STAT6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAT6
• Synonyms: D12S1644; IL-4-STAT; STAT6B; STAT6C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003153 edited
  ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC
  GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG
  TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT
  GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC
  ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA
  CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT
  TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA
  GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG
  TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC
  ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG
  ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC
  CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG
  GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG
  CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC
  CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG
  TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG
  GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC
  ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC
  AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA
  GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC
  ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC
  AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT
  GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG
  GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG
  ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG
  CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT
  GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC
  TTCATCAGCAAACAGTACGTTACTAGCTTTCTTCTCAATGAGCCCGACGGAACCTTTCTC
  CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG
  GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC
  TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC
  AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT
  TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG
  CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG
  AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC
  CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG
  CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC
  CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG
  CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT
  CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG
  GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA
  CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC
  CTAAGGGCCAACCCCAGTTGGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003153 unedited
  GTTCAGATTTGTATACGACTCACTATAGGCGGCCGACGAATTCGCACGAGGTGAAGAGGG
  AGGACGGGAGAGGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTGTGTGCTT
  TATCTTATTTTTCTTTTTGGTGGTGGTGGTGGAAGGGGGGAGGTGCTAGCAGGGCCAGCC
  TTGAACTCGCTGGACAGAGCTACAGACCTATGGGGCCTGGAAGTGCCCGCTGAGAAAGGG
  AGAAGACAGCAGAGGGGTTGCCGAGGCAACCTCCAAGTCCCAGATCATGTCTCTGTGGGG
  TCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTCGACTTTCCCCAACA
  CCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAGTTCCTGGTCGGCTC
  CGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACTGTCCAGCACCTTCA
  GGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACACATCAGCACCCTTGA
  GAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAAATACTTCAAGG
  AGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCA
  GGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCA
  CCTTCTCCGAGAAGCCCTGCAGAAGGAGGCTGANGCTGGCCAAGTGTCTCTGCACAGCTT
  GATAGAAACTCCTGCNTATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCANGA
  GACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTNGAGAGGATCCAAATTNGAAA
  ACGCAGCANCAGCTGGCAGGGAATGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003153 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGGGCATGCAACTAAGGTGCCA
  GCTATATATTTAACATATCCTAGGTACATACACGTTCACACAGCTATACACGAAGAATCT
  CAGCCCTTGTACTTTTGCATAGTCTCATACACGTATCAAAAGCCTCCACCTGGCTAACAG
  GAATTTGGGGCTTTGGGAGATTTTTTAATCAGGGCAAAACCTGTACTAGTAACCACATGT
  CCAGACTCCTCCTATGCTCCCACCCAGGGTCCCTTGAGCTGCTTCCCATTCCCCTAGGGC
  TGAGACCCAATATCCTCTATCCCTGGCCTCTAGTGTAAATGTGTCTGTATGTTCCTGCCT
  ATCCGTCCTAAGCCCTGGGTCAGAATGGGCGGAGAAGCCTTCCATGCCCTAACCTGTGCT
  CTTACCCAGCCCCCCTCCTGCTCAGCCCCATCACCCTCAGAGAGCTCTGTATGTGTGTGT
  GCGTGCGTGTGCGCGCTGCAAGTGCAGGCATGTTGGGGCGTGTCTCAAAGCCTGAACTTC
  CCTTCCANTCANTGCTGGAAGGAAGTGGGCAGGGGAATGATTCAAAGGAAGGAGTGGATT
  GGCTCCACCCACTGTGCATTCTCCTGTTAGTCTTTTCCTCCTGACCCACGAGTANGTGGG
  GATAGGAAGGCACCCTTNCCATCTGCTGCTTGGCAAGGCATGACCCAGTGTCCATAGCAG
  GTCTGTGGGGGTAAGANAAAAACTGGTTCTTTGGGTTCTCCTTCAGCTGGGATCAACAAC
  TGGGGTTGGCCCTTAGGTCCATGTGGGAAATTGGAATCCCAAATTGCCCCTATTGGAAAG
  GCTGCAGGAGGGGCTTTGCCCCCCAGGAACCTTCCCCCGAATCCCTTGGGCCCCCAAGAG
  AACTTATGGAGGCCCGTTCCATGGAAGCGCGCAAGGAGGAATTGTTTTCACAACCAACGC
  CTTAGGAACCCGGACTGCTTGGTCAGCACTGTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003153
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003153.3, NP_003144.3
• RefSeq Size: 3993 bp
• RefSeq ORF: 2544 bp
• Locus ID: 6778
• UniProt ID: P42226
• Cytogenetics: 12q13.3
• Domains: SH2, STAT
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.