SPR (NM_003124) Human Untagged Clone

CAT#: SC118171

SPR (untagged)-Human sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPR
• Synonyms: SDR38C1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118171 sequence for NM_003124 edited (data generated by NextGen Sequencing)
  ATGGAGGGCGGGCTGGGGCGTGCTGTGTGCTTGCTGACCGGGGCCTCCCGCGGCTTCGGC
  CGGACGCTGGCCCCGCTCCTGGCCTCGCTGCTGTCGCCCGGCTCCGTGCTTGTCCTTAGC
  GCCCGCAACGACGAGGCACTGCGCCAGCTGGAGGCCGAGCTGGGCGCCGAGCGGTCTGGC
  CTGCGCGTGGTGCGGGTGCCCGCCGACCTGGGCGCCGAGGCCGGCTTGCAGCAGCTGCTC
  GGCGCCCTGCGCGAGCTCCCCCGGCCCAAGGGGCTGCAGCGACTGCTGCTTATCAACAAC
  GCGGGCTCTCTTGGGGATGTGTCCAAAGGCTTCGTGGACCTGAGTGACTCCACTCAAGTG
  AACAACTACTGGGCACTGAACTTGACCTCCATGCTCTGCCTGACTTCCAGCGTCCTGAAG
  GCCTTCCCGGACAGTCCTGGCCTCAACAGAACCGTGGTTAACATCTCGTCCCTCTGTGCC
  CTGCAACCTTTCAAAGGCTGGGCGCTGTACTGTGCAGGAAAGGCTGCTCGTGATATGCTG
  TTCCAGGTCCTGGCGCTGGAGGAACCTAATGTGAGGGTGCTGAACTATGCCCCAGGTCCT
  CTGGACACAGACATGCAGCAGTTGGCCCGGGAGACCTCCGTGGACCCAGACATGCGAAAA
  GGGCTGCAGGAGCTGAAGGCAAAGGGGAAGCTGGTGGATTGCAAGGTGTCAGCCCAGAAA
  CTGCTGAGCTTACTGGAAAAGGACGAGTTCAAGTCTGGAGCCCACGTGGACTTCTATGAC
  AAATAA
  
  Clone variation with respect to NM_003124.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003124 unedited
  AGTCAAACATTGTAAACGACTCACTATAGGCGGCCGCGACATCGGCACGAGCCGCCGGCG
  GAGAACAGAGCATGGAGGGCGGGCTGGGGCGTGCTGTGTGCTTGCTGACCGGGGCCTCCC
  GCGGCTTCGGCCGGACGCTGGCCCCGCTCCTGGCCTCGCTGCTGTCGCCCGGCTCCGTGC
  TTGTCCTTAGCGCCCGCAACGACGAGGCACTGCGCCAGCTGGAGGCCGAGCTGGGCGCCG
  AGCGGTCTGGCCTGCGCGTGGTGCGGGTGCCCGCCGACCTGGGCGCCGAGGCCGGCTTGC
  AGCAGCTGCTCGGCGCCCTGCGCGAGCTCCCCCGGCCCAAGGGGCTGCAGCGACTGCTGC
  TTATCAACAACGCGGGCTCTCTTGGGGATGTGTCCAAAGGCTTCGTGGACCTGAGTGACT
  CCACTCAAGTGAACAACTACTGGGCACTGAACTTGACCTCCATGCTCTGCCTGACTTCCA
  GCGTCCTGAAGGCCTTCCCGGACAGTCCTGGCCTCAACAGAACCGTGGTTAACATCTCGT
  CCCTCTGTGCCCTGCAACCTTTCAAAGGCTGGGCGCTGTACTGTGCAGGAAAGGCTGCTC
  GTGATATGCTGTTCCAGGTCCTGGCGCTGGAGGAACCTAATGTGAGGGTGCTGAACTATG
  CCCCAGGTCCTCTGGACACAGACATGCAGCAGTTGGCCCGGGAGACCTCCGTGGACCCAG
  ACATGCGAAAAGGGCTGCAGGAGCTGAAGGCAAAGGGGAAGCTTGTGGATTGCAAGGTGT
  CAGCCCAGAACTGCTGAGCTTACTGGAAANGNACGAGTTCAAGTCTTGAGCCACGTGGAC
  TTCTATTGACAATAAGCCATGTTTTTNGCTTCCTGACCTTTTTGCCCCACTTTTAGACTA
  CCCAGAGCCCTGTGCTCCCCACACCTGCCTANGGGCAGTCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003124
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003124.3, NP_003115.1
• RefSeq Size: 1438 bp
• RefSeq ORF: 786 bp
• Locus ID: 6697
• UniProt ID: P35270
• Cytogenetics: 2p13.2
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008]