Sorbitol Dehydrogenase (SORD) (NM_003104) Human Untagged Clone

CAT#: SC118158

SORD (untagged)-Human sorbitol dehydrogenase (SORD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Sorbitol Dehydrogenase
• Synonyms: HEL-S-95n; RDH; SDH; SORD1; SORDD; XDH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003104, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCGGCCAAGCCCAACAACCTTTCCCTGGTGGTGCACGGACCGGGGGACTTGCGCCTGGAGA
  ACTATCCTATCCCTGAACCAGGCCCAAATGAGGTCTTGCTGAGGATGCATTCTGTTGGAATCTGTGGCTC
  AGATGTCCACTACTGGGAGTATGGTCGAATTGGGAATTTTATTGTGAAAAAGCCCATGGTGCTGGGACAT
  GAAGCTTCGGGAACAGTCGAAAAAGTGGGATCATCGGTAAAGCACCTAAAACCAGGTGATCGTGTTGCCA
  TCGAGCCTGGTGCTCCCCGAGAAAATGATGAATTCTGCAAGATGGGCCGATACAATCTGTCACCTTCCAT
  CTTCTTCTGTGCCACGCCCCCCGATGACGGGAACCTCTGCCGGTTCTATAAGCACAATGCAGCCTTTTGT
  TACAAGCTTCCTGACAATGTCACCTTTGAGGAAGGCGCCCTGATCGAGCCACTTTCTGTGGGGATCCATG
  CCTGCAGGAGAGGCGGAGTTACCCTGGGACACAAGGTCCTTGTGTGTGGAGCTGGGCCAATCGGGATGGT
  CACTTTGCTCGTGGCCAAAGCAATGGGAGCAGCTCAAGTAGTGGTGACTGATCTGTCTGCTACCCGATTG
  TCCAAAGCCAAGGAGATTGGGGCTGATTTAGTCCTCCAGATCTCCAAGGAGAGCCCTCAGGAAATCGCCA
  GGAAAGTAGAAGGTCAGCTGGGGTGCAAGCCGGAAGTCACCATCGAGTGCACGGGGGCAGAGGCCTCCAT
  CCAGGCGGGCATCTACGCCACTCGCTCTGGTGGGAACCTCGTGCTTGTGGGGCTGGGCTCTGAGATGACC
  ACCGTACCCCTACTGCATGCAGCCATCCGGGAGGTGGATATCAAGGGCGTGTTTCGATACTGCAACACGT
  GGCCAGTGGCGATTTCGATGCTTGCGTCCAAGTCTGTGAATGTAAAACCCCTCGTCACCCATAGGTTTCC
  TCTGGAGAAAGCTCTGGAGGCCTTTGAAACATTTAAAAAGGGATTGGGGTTGAAAATCATGCTCAAGTGT
  GACCCCAGTGACCAGAATCCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003104 unedited
  GGTATATTTGTCAATACGCACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCAGA
  GCGACCAAACGTCCCGCGCCTTCCAGGCCGCACTCCAGAGCCAAAAGAGCTCCATGGCGG
  CGGCGGCCAAGCCCAACAACCTTTCCCTGGTGGTGCACGGACCGGGGGACTTGCGCCTGG
  AGAACTATCCTATCCCTGAACCAGGCCCAAATGAGGTCTTGCTGAGGATGCATTCTGTTG
  GAATCTGTGGCTCAGATGTCCACTACTGGGAGTATGGTCGAATTGGGAATTTTATTGTGA
  AAAAGCCCATGGTGCTGGGACATGAAGCTTCGGGAACAGTCGAAAAAGTGGGATCATCGG
  TAAAGCACCTAAAACCAGGTGATCGTGTTGCCATCGAGCCTGGTGCTCCCCGAGAAAATG
  ATGAATTCTGCAAGATGGGCCGATACAATCTGTCACCTTCCATCTTCTTCTGTGCCACGC
  CCCCCGATGACGGGAACCTCTGCCGGTTCTATAAGCACAATGCAGCCTTTTGTTACAAGC
  TTCCTGACAATGTCACCTTTGAGGAAGGCGCCCTGATCGAGCCACTTTCTGTGGGGATCC
  ATGCCTGCAGGAGAGGCGGAGTTACCCTGNGACACAAGGTCCTTTGTGTGTGGAGCTGGG
  CCAATCGGGATGGTCACTTTGCTCGTGGCCAAAGCAATGGGAGCAGCTCAAGTAGTGGTG
  ACTGATCTGTCTGCTACCCCGATTGCCAAAGCCAAGGAGAAGGGNGCTGATTTACTCCTC
  CAGATCTCCAAGGAAGCCCTCAGAAAACCGCCAGGAAAGTAAAAGTCAGCTGGGGTGCCA
  GCCGGAATCACCATCGAGTGCACGGGGCAGAGCCTCCATCCAGGCGGGCATTACGCCACT
  CGCTCTGTGGGACCATCTTGCTTGTGGGGCTGGCTCTTAAAGACACCGAACCCCTATGAT
  GCGGCCTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003104 unedited
  NGGTACCACCACCAATATTTATTACCCCAAGNNCAATCTGAAGAATCGGGCACAAGGGTC
  ATGGCTTTCCCAGGTTACAAGAAAAATCAGAACCTCTTCAAACTCACCCTCCACCCATAG
  CCAAAGTAAGGAATTAAAGGCTATAGCTCAAGCTATGTCATGGCTACTATATCCTCATTC
  TTGATGAATAGTCTCAAAGTCACAAGTTGGCATGATGCGATTTAGGTCTAAATCTTACCA
  GAAAAAAAACCCACCATTAAAATAAGACAAGTTCATAGAAATCATTCCAACAGAAAGATA
  CCAAGAGCCTTTACTGGTAATGAGAAGAGGAAAGATTTTCTGTTTATAATAACCAGAAAG
  AAATGGAATTGTCTAATAACCATCAATTGAGTATGAGCCTAGTTGAGTTGAGTATGAGTC
  TAGTATGGGATGCTAATCTTCTGGGGTGGGGGAAGTCCTGTGAGCTAACCAAACCCTCCT
  GGTCTCTGGTGAGGTCCACAAGTAGCAGTTTCCTGGTTGCCTGGTGACAAGGGACCATGC
  TTAGGGGTGGGCTGGGGCATGTTGGGGAGGGGGTCCTTCACTTGGCAAGTGAAATAACAC
  AATTCTCAGAAGCTGTAGAAGAGGCAATCTTCAAACAGTGCTGAGTAGTCCTCTTACTTT
  TTCCACATTANGGGAATGCAAACTGGTTTAACCTCACATGACATTTCCTTTTTTTGTTGT
  TTGTTTTTTCATTTGATTGGTTTAAAAATTATCCTGGATCCANGAAAGTGATGCCCTTTT
  CTTATTCTCCTTTTATCATCAAAACANACAGNCCATATGTTCTCTAGAAACATTGCCGTT
  CTAAACCGGAAGTGCTGACCAGCCTTCCATCCAGACCCTGAGTGGCAGATTTCATGAAAT
  AAACCTTCGGGCCAGTTAAGTCTCTCTGCAGCTTTCTTTTCTTACTCAACCCTCATTCCC
  TTTGGGGCANAGCAGGGGAACCGAAAACCCAGGGCCCATTTCCTTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003104
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003104.3, NP_003095.1
• RefSeq Size: 2628 bp
• RefSeq ORF: 1074 bp
• Locus ID: 6652
• UniProt ID: Q00796
• Cytogenetics: 15q21.1
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
  Transcript Variant: This variant (1) represents the protein-coding transcript.