Troponin C (TNNC2) (NM_003279) Human Untagged Clone

CAT#: SC118101

TNNC2 (untagged)-Human troponin C type 2 (fast) (TNNC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Troponin C
• Synonyms: CFAP85; FAP85
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118101 sequence for NM_003279 edited (data generated by NextGen Sequencing)
  ATGACGGACCAGCAGGCTGAGGCCAGGTCCTACCTCAGCGAAGAGATGATCGCTGAGTTC
  AAGGCTGCCTTTGACATGTTTGATGCTGATGGTGGTGGGGACATCAGCGTCAAGGAGTTG
  GGCACGGTGATGAGGATGCTGGGCCAGACACCCACCAAGGAGGAGCTGGACGCCATCATC
  GAGGAGGTGGATGAGGACGGCAGCGGCACCATCGACTTCGAGGAGTTCTTGGTCATGATG
  GTGCGCCAGATGAAAGAGGACGCGAAAGGGAAGAGCGAGGAGGAGCTGGCCGAGTGCTTC
  CGCATCTTCGACAGGAATGCAGACGGCTACATCGACCCGGAGGAGCTGGCTGAGATTTTC
  AGGGCCTCCGGGGAGCACGTGACTGACGAGGAGATCGAATCTCTGATGAAAGACGGCGAC
  AAGAACAACGACGGCCGCATTGACTTCGACGAGTTCCTGAAGATGATGGAGGGCGTGCAG
  TAA
  
  Clone variation with respect to NM_003279.2
  384 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003279 unedited
  GGTTANAATTTGGATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTGCAAAGG
  AGGCGACCTGCAACAGAGGAGTCCCGGTCACCAGCAACCATGACGGACCAGCAGGCTGAG
  GCCAGGTCCTACCTCAGCGAAGAGATGATCGCTGAGTTCAAGGCTGCCTTTGACATGTTT
  GATGCTGATGGTGGTGGGGACATCAGCGTCAAGGAGTTGGGCACGGTGATGAGGATGCTG
  GGCCAGACACCCACCAAGGAGGAGCTGGACGCCATCATCGAGGAGGTGGATGAGGACGGC
  AGCGGCACCATCGACTTCGAGGAGTTCTTGGTCATGATGGTGCGCCAGATGAAAGAGGAC
  GCGAAAGGGAAGAGCGAGGAGGAGCTGGCCGAGTGCTTCCGCATCTTCGACAGGAATGCA
  GACGGCTACATCGACCCGGAGGAGCTGGCTGAGATTTTCAGGGCCTCCGGGGAGCACGTG
  ACTGACGAGGAGATCGAATCTCTGATGAAAGACGGCGACAAGAACAACGACGGCCGCATT
  GACTTCGACGAGTTCCTGAAGATGATGGAGGGCGTGCAGTAAGGAGTGGACAGTCCGCCC
  CTACCAAGATCGCGTGGCCCTAAGGTGTCGGGAGACTCCGCCCTGCCGGGTCCCCCACCA
  GGGAGGCGCGCGCCCTTGTGGGTCTTTGGCTGGAAGGAATAAATGCAAATGTTCCAAACC
  TCGTGGCCTGAATGAGCGGAAGCCCAGGACCCCACGTCGCAAGGGCTGTGCGTCAGGGGG
  CCATTCCGGCTTGGCACCACCCCGGACCACCCTTGGGCACCCCCGGAGAGGCTGCCTTAC
  CGGCGGCATGTGGTAGGAACCCGGGCCCTTGTATCCCCAGGCGGCCCCCTGCCCACTCTT
  CCATGGCGCTCGACTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003279
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003279.2, NP_003270.1
• RefSeq Size: 698 bp
• RefSeq ORF: 483 bp
• Locus ID: 7125
• UniProt ID: P02585
• Cytogenetics: 20q13.12
• Domains: EFh
• Protein Pathways: Calcium signaling pathway
• Gene Summary: Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq, Jul 2008]