H2AC18 (NM_003516) Human Untagged Clone

CAT#: SC117916

HIST2H2AA3 (untagged)-Human histone cluster 2, H2aa3 (HIST2H2AA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H2AC18
• Synonyms: H2A; H2a-615; H2A.2; H2A/O; H2A/q; H2AC19; H2AFO; HIST2H2AA; HIST2H2AA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003516 edited
  ATGTCTGGTCGTGGCAAGCAAGGAGGCAAGGCCCGCGCCAAGGCCAAGTCGCGCTCGTCC
  CGCGCTGGCCTTCAGTTCCCGGTAGGGCGAGTGCATCGCTTGCTGCGCAAAGGCAACTAC
  GCGGAGCGAGTGGGGGCCGGCGCGCCCGTCTACATGGCTGCGGTCCTCGAGTATCTGACC
  GCCGAGATCCTGGAGCTGGCGGGCAACGCGGCTCGGGACAACAAGAAGACGCGCATCATC
  CCTCGTCACCTCCAGCTGGCCATCCGCAACGACGAGGAACTGAACAAGCTGCTGGGCAAA
  GTCACCATCGCCCAGGGCGGCGTCTTGCCTAACATCCAGGCCGTACTGCTCCCTAAGAAG
  ACGGAGAGTCACCACAAGGCAAAGGGCAAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003516 unedited
  GGTTGATTATTTTAGTAATACGATTTACTATAGGGCGGNCGCGAATTCGGACGAGGGAGT
  TTCTCTCGGTGACTACTATCGCTGTCATGTCTGGTCGTGGCAAGCAAGGAGGCAAGGCCC
  GCGCCAAGGCCAAGTCGCGCTCGTCCCGCGCTGGCCTTCAGTTCCCGGTAGGGCGAGTGC
  ATCGCTTGCTGCGCAAAGGCAACTACGCGGAGCGAGTGGGGGCCGGCGCGCCCGTCTACA
  TGGCTGCGGTCCTCGAGTATCTGACCGCCGAGATCCTGGAGCTGGCGGGCAACGCGGCTC
  GGGACAACAAGAAGACGCGCATCATCCCTCGTCACCTCCAGCTGGCCATCCGCAACGACG
  AGGAACTGAACAAGCTGCTGGGCAAAGTCACCATCGCCCAAGGCGGTGTCTTGTCTAACA
  TCCAGGCCCGTACTGCTCCCTAAGAAAACGGACAGTCACCACAAGGCAAAGGGCAAGCGA
  AGCTGACCTCCGGCCCAAGTGGGCCCAGCCCGGCCCGCGTCTCCAAGCGCACCCGTGAAC
  TCAAAAGGCTCTCTTTAGAGCCCCCCACCTTTTTCAATACAAGAAGTCGTTATGCCGCGC
  ACTCTCCCAAAAAAAACACAAACCTCCACTCTTAATTGCGGCCGCGGCCATACCTGCCCC
  CTGAAAAGATCCCGGTTGGATTCCTTGGACCCCTCCCCAAGCCTTACTGGCCCTGCAATC
  GCCACTCCATGCCCCCCAACCTTACCTAACAAACTAAATGGATCCTTTCCCCGAACAGCG
  GACCTTCTAATACACGGGACAAAGGCGAGCACTCACCACGAGAAGCTGGACACAACCGTA
  GGCCGCGCGGTATAGGCACCCCCGCCGCACGCAACACCTGACCTTGAACCCTGCCCTCGG
  TCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003516 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGAGTGACCAGCATTAAC
  AACTCTTTTATTTGAAAACGTGGGTGGCTCTGAAAAGAGCCTTTTGAGTTCACAGGTGCC
  CCTTCGAGACGCGGGCCGGGCTGGGCCCACTTGGGCCGGACGTCAGCCTCACTTGCCCTT
  TGCCTTGTGGTGACTCTCCGTCTTCTTAGGGAGCAGTACGGCCTGGATGTTAGGCAAGAC
  GCCGCCCTGGGCGATGGTGACTTTGCCCAGCAGCTTGTTCAGTTCCTCGTCGTTGCGGAT
  GGCCAGCTGGAGGTGACGAGGGATGATGCGCGTCTTCTTGTTGTCCCGAGCCGCGTTGCC
  CGCCAGCTCCAGGATCTCGGCGGTCAGATACTCGAGGACCGCAGCCATGTAGACGGGCGC
  GCCGGCCCCCACTCGCTCCGCGTAGTTGCCTTTGCGCAGCAAGCGATGCACTCGCCCTAC
  CGGGAACTGAAGGCCAGCGCGGGACGAGCGCGACTTGGCCTTGGCGCGGGCCTTGCCTCC
  TTGCTTGCCACGACCAGACATGACAGCGATAGTAGTCACCGAGAGAAACTCCCCTCGTGC
  CGAATTCGCGGCCGCCCTATAGTGAGTCGTAATACAAAAATTCTGACGGGTCACTAAACG
  AGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTAACCGGGCG
  GGGTTATTACGACCTTTGGAAAGCCCCGTGATTTTGGGGCAAAACAAACCCCCTTGCCGG
  AATGGGGGGGGACTGGGAAACCCCGGGAGCAAACGCTTTCCCGCCATTGTGGACTGCCAA
  ACCCATTCCCTGGGATACCGAGACTAACCTAAAGGCCTGCCCATCGAAAGTCCGTACGGC
  TGTACTGGCAAAAGCCGGGGGCCCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003516
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003516.2, NP_003507.1
• RefSeq Size: 534 bp
• RefSeq ORF: 393 bp
• Locus ID: 8337
• UniProt ID: Q6FI13
• Cytogenetics: 1q21.2
• Domains: H2A, histone
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015]