Macrophage inflammatory protein 5 (CCL15) (NM_004167) Human Untagged Clone

CAT#: SC117560

CCL15 (untagged)-Human chemokine (C-C motif) ligand 15 (CCL15), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Macrophage inflammatory protein 5
• Synonyms: HCC-2; HMRP-2B; Lkn-1; LKN1; MIP-1d; MIP-5; NCC-3; NCC3; SCYA15; SCYL3; SY15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117560 sequence for NM_004167 edited (data generated by NextGen Sequencing)
  ATGAAGGTCTCCGTGGCTGCCCTCTCCTGCCTCATGCTTGTTGCTGTCCTTGGATCCCAG
  GCCCAGTTCATAAATGATGCAGAGACAGAGTTAATGATGTCAAAGCTTCCACTGGAAAAT
  CCAGTAGTTCTGAACAGCTTTCACTTTGCTGCTGACTGCTGCACCTCCTACATCTCACAA
  AGCATCCCGTGTTCACTCATGAAAAGTTATTTTGAAACGAGCAGCGAGTGCTCCAAGCCA
  GGTGTCATATTCCTCACCAAGAAGGGGCGGCAAGTCTGTGCCAAACCCAGTGGTCCGGGA
  GTTCAGGATTGCATGAAAAAGCTGAAGCCCTACTCAATATAA
  
  Clone variation with respect to NM_004167.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004167 unedited
  NGTGTCANAATTTGTATACGACTCCTATAGGCGGCCGCGATTCGCCCACGGAATAGACTG
  GAAGGGAAAAAGGCAGCATTCACCACATCCCAATCCTGAATCCAAGAGTCTAAGATAGTC
  CCCCACTCCTATCTCAGGCTTAGAGGATTAGATTAATCTCCTGGAGGGAAGACTCTTCCT
  TGAAACATTTTTTTTTATCTGCCTGTAGCTATTGGGATAATTCGGGAAATCCACAGGGAC
  AGTTCAAGTCATCTTTGTCCTCTACTTTCTGTTGCACTCTCAGCCTTGTTCTCTTTTTAG
  AAACTGCATGGTAACTATTATATAGCTAAAGAAGAGCATTCTGACCTCTGCCCTGGGACT
  TCCTGGATCCTCCTCTTCTTATAAATACAAGGGCAGAGCTGGTATCCCGGGGAGCCAGGA
  AGCAGTGAGCCCAGGAGTCCTCGGCCAGCCCTGCCTGCCCACCAGGAGGATGAAGGTCTC
  CGTGGCTGCCCTCTCCTGCCTCATGCTTGTTGCTGTCCTTGGATCCCAGGCCCAGTTCAC
  AAATGATGCAGAGACAGAGTTAATGATGTCAAAGCTTCCACTGGAAAATCCAGTAGTTCT
  GAACAGCTTTCACTTTGCTGCTGACTGCTGCACCTCCTACATCTCACAAAGCATCCCGTG
  TTCACTCATGAAAAGTTATTTTGAAACGAGCAGCGAGTGCTCCAAGCCAGGTGTCATATT
  CCTCACCAAGAAGGGCGGCAAGTCTGTGCCAAACCCAGTGGTCCGGGAGTTCANGATTGC
  ATGANAAAGCTGAAGCCCCTACTCATATANNTATAAAGAGACCAAAGAGCCAGNCACCCA
  CTNCACACCTNCTGAGCCTCTGAAGCTCCACCCAGCCAGCTCTTCTNCCACACAGCTTCC
  ACAGCATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004167 unedited
  TGAACCGCGGCACCGCATTCTAGTAGTCGAGNNCTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTGGGNAAAAAACCAGAATGGGCTTTTATTCAAAACACGGA
  AACCCCCAAAAGGGGACTGGGGCCTAAAATTAACCGGGGGGGGAAGAAGGGGCCCTGGCA
  TCTTCTCTTTATTGCTCTGAACTGGGCCCTTGCCACCCCTTTTGGGGAACTCAAATCTCC
  CTCATGTCCCTGATATAATCCCGGACCCCCTTTTCACTGGGGGTGGGACAAACCGAATGG
  CCCCTTTTGGGGAAAAAAACAATTCCCGGCTTGGAACACCGGCTGGTGGGCCCATAATAA
  TCCCTAATCCCCTTACCCCGGATCTTTTAAGGAATGTAAGGGAAACAACCCCCTTAAGGG
  GGGGAAAGGCCCCCCGAAGAAGATTTAATCTTGGTCCCCAAGGCCATTGTGATTAAGAAG
  AAAAAAGGAATGGCCTCCCCCGAAATCTTTATGCCGGGGGAAACCTTTTTGGGAAGAAAA
  CTGGCCCGCGGGGAACCTCAAAAGCCCAAGAAGGGTTTGAAGGGGGTGGGCTGCCCCCTT
  TGGCCTTTTATTTTAATATGAGAAGGGGCTTTAACTTTTTTATGCCAATCCCAAAACCCC
  CGGACCCTTGGTTTTGGCACAAACCTTTCCCGCCCCTTTTTGGGAAGGAAATTGACACCC
  GGCTTCGGAAACCCCCCCGCCCCTTACAAAAAAATCTTTTAGAATTAAAACCAGCATTCT
  CTCTCGAATATTTCCAAGTGCCTCCCCCCCCCACCAACTTAAAACCCGTGCAAAACACCC
  TGGATTTTCCATAGAAAGTTTGACACTTATACCTCTGCCTTTGACCATGCGGAAACTGGG
  CCCGGTTCCCCGCTAATCACCACCTCTGGCTGTATATGCTGCCCGCGAACCCTCATTCTT
  TTGGGGCCACCTACTCGTCCGGAGCACCGGTCTTTTCTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004167
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004167.3, NP_004158.2
• RefSeq Size: 1392 bp
• RefSeq ORF: 342 bp
• Locus ID: 6359
• Cytogenetics: 17q12
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (2) is a bicistronic transcript that includes the longer form of the downstream gene CCL14.