COX5A (NM_004255) Human Untagged Clone

CAT#: SC117461

COX5A (untagged)-Human cytochrome c oxidase subunit Va (COX5A), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COX5A
• Synonyms: COX; COX-VA; MC4DN20; VA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004255 edited
  ATGCTGGGCGCCGCTCTCCGCCGCTGCGCTGTGGCCGCAACCACCCGGGCCGACCCTCGA
  GGCCTCCTGCACTCCGCCCGGACCCCCGGCCCCGCCGTGGCTATCCAGTCAGTTCGCTGC
  TATTCCCATGGGTCACAGGAGACAGATGAGGAGTTTGATGCTCGCTGGGTAACATACTTC
  AACAAGCCAGATATAGATGCCTGGGAATTGCGTAAAGGGATAAACACACTTGTTACCTAT
  GATATGGTTCCAGAGCCCAAAATCATTGATGCTGCTTTGCGGGCATGCAGACGGTTAAAT
  GATTTTGCTAGTACAGTTCGTATCCTAGAGGTTGTTAAGGACAAAGCAGGACCTCATAAG
  GAAATCTACCCCTATGTCATCCAGGAACTTAGACCAACTTTAAATGAACTGGGAATCTCC
  ACTCCGGAGGAACTGGGCCTTGACAAAGTGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004255 unedited
  CACAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCCGCCATCGCCG
  TCATGCTGGGCGCCGCTCTCCGCCGCTGCGCTGTGGCCGCAACCACCCGGGCCGACCCTC
  GAGGCCTCCTGCACTCCGCCCGGACCCCCGGCCCCGCCGTGGCTATCCAGTCAGTTCGCT
  GCTATTCCCATGGGTCACAGGAGACAGATGAGGAGTTTGATGCTCGCTGGGTAACATACT
  TCAACAAGCCAGATATAGATGCCTGGGAATTGCGTAAAGGGATAAACACACTTGTTACCT
  ATGATATGGTTCCAGAGCCCAAAATCATTGATGCTGCTTTGCGGGCATGCAGACGGTTAA
  ATGATTTTGCTAGTACAGTTCGTATCCTAGAGGTTGTTAAGGACAAAGCAGGACCTCATA
  AGGAAATCTACCCCTATGTCATCCAGGAACTTAGACCAACTTTAAATGAACTGGGAATCT
  CCACTCCGGAGGAACTGGGCCTTGACAAAGTGTAAACCGCATGGATGGGCTTCCCCCAAG
  GATTTATTGACATTGCTACTTGAGTGTGAACAGTTACCTGGAAATACTGATGATAACATA
  TTACCTTATTTGAACAAGTTTTCCTTTATTGAGTACCAAGCCATGTAATGGTAACTTGGA
  CTNTAATAAAAGGGAAATGAGTTTGAACTGAAAAAAAAAAAAAAAAACTCGACTCTAGAT
  TGCGGCCGCGGTCATAGCTGTTTCCTGACCAGATCCCGGTTGGCATCCCTGTGACCCCTC
  CCCAGTGCCTNTCCTGGCCCTGGAGGTGCCACTCCAGGGCCACCAGCCTTGCCCTATAAA
  ATAAGTGCATCATTTTGCCTGACTAGTGTCCCTCTAAATATATGGGTGAGCGGGGGGGAA
  TTGGACCAGGGCCCACCGGCAACACACCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004255 unedited
  CACTTCCAGGGCCGGNAAAGCACTGGGGNAGGGGTCACAGGGATGCCACCCGGGATCTGT
  TCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTC
  AGTTCAAACTCATTTCCCTTTTATTAAAGTCCAAGTTACCATTACATGGCTTGGTACTCA
  ATAAAGGAAAACTTGTTCAAATAAGGTAATATGTTATCATCAGTATTTCCAGGTAACTGT
  TCACACTCAAGTAGCAATGTCAATAAATCCTTGGGGAAGCCCATCCATGCGGTTTACACT
  TTGTCAAGGCCCAGTTCCTCCGGAGTGGAGATTCCCAGTTCATTTAAAGTTGGTCTAAGT
  TCCTGGATGACATAGGGGTAGATTTCCTTATGAGGTCCTGCTTTGTCCTTAACAACTNNN
  ATTGATACGAACTGTACTAGCAAAATCATTTAACCGTCTGCATGCCCGCAAAGCAGCATC
  AATGATTTTGGGCTCTGGAACCATATCATAGGTAACAAGTGTGTTTATCCCTTTACGCAA
  TTCCCAGGCATCTATATCTGGCTTGTTGAAGTATGTTACCCAGCGAGCATCAAACTCCTC
  ATCTGTCTCCTGTGACCCATGGGAATAGCAGCGAACTGACTGGATAGCCACGGCGGGGCC
  GGNGGTCCCGGCCGAGTGCAGGAGGCCTCNAGGGTCGGCCCGGTTGGTTGCGGCCACAGC
  GCAGCGGCGGAATCCTCTTNNNATAGAGGGGAAAGGCCGGCCTCGGGCGAATTCCCGGGC
  GCCCTTATAGGGAGTCGTATACAAATTCTTGACGGTTACTAAACGAGCTCTGCTTATAAG
  ACCTCNACCGTACCGCTTAACGCCTATTGCGTACGGGGGCGGGTTATTACAATTTGAAAG
  ACCCTGATTTGGTGCAACAACTCCATGCGTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004255
• Insert Size: 4680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004255.2, NP_004246.1
• RefSeq Size: 645 bp
• RefSeq ORF: 453 bp
• Locus ID: 9377
• UniProt ID: P20674
• Cytogenetics: 15q24.2
• Domains: COX5A
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008]