PCK2 (NM_004563) Human Untagged Clone

CAT#: SC117281

PCK2 (untagged)-Human phosphoenolpyruvate carboxykinase 2 (mitochondrial) (PCK2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCK2
• Synonyms: PEPCK; PEPCK-M; PEPCK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117281 sequence for NM_004563 edited (data generated by NextGen Sequencing)
  ATGGCCGCATTGTACCGCCCTGGCCTGCGGCTTAACTGGCATGGGCTGAGCCCCTTGGGC
  TGGCCATCATGCCGTAGCATCCAGACCCTGCGAGTGCTTAGTGGAGATCTGGGCCAGCTT
  CCCACTGGCATTCGAGATTTTGTAGAGCACAGTGCCCGCCTGTGCCAACCAGAGGGCATC
  CACATCTGTGATGGAACTGAGGCTGAGAATACTGCCACACTGACCCTGCTGGAGCAGCAG
  GGCCTCATCCGAAAGCTCCCCAAGTACAATAACTGCTGGCTGGCCCGCACAGACCCCAAG
  GATGTGGCACGAGTAGAGAGCAAGACGGTGATTGTAACTCCTTCTCAGCGGGACACGGTA
  CCACTCCCGCCTGGTGGGGCCCGTGGGCAGCTGGGCAACTGGATGTCCCCAGCTGATTTC
  CAGCGAGCTGTGGATGAGAGGTTTCCAGGCTGCATGCAGGGCCGCACCATGTATGTGCTT
  CCATTCAGCATGGGTCCTGTGGGCTCCCCGCTGTCCCGCATCGGGGTGCAGCTCACTGAC
  TCAGCCTATGTGGTGGCAAGCATGCGTATTATGACCCGACTGGGGACACCTGTGCTTCAG
  GCCCTGGGAGATGGTGACTTTGTCAAGTGTCTGCACTCCGTGGGCCAGCCCCTGACAGGA
  CAAGGGGAGCCAGTGAGCCAGTGGCCGTGCAACCCAGAGAAAACCCTGATTGGCCACGTG
  CCCGACCAGCGGGAGATCATCTCCTTCGGCAGCGGCTATGGTGGCAACTCCCTGCTGGGC
  AAGAAGTGCTTTGCCCTACGCATCGCCTCTCGGCTGGCCCGGGATGAGGGCTGGCTGGCA
  GAGCACATGCTGATCCTGGGCATCACCAGCCCTGCAGGGAAGAAGCGCTATGTGGCAGCC
  GCCTTCCCTAGTGCCTGTGGCAAGACCAACCTGGCTATGATGCGGCCTGCACTGCCAGGC
  TGGAAAGTGGAGTGTGTGGGGGATGATATTGCTTGGATGAGGTTTGACAGTGAAGGTCGA
  CTCCGGGCCATCAACCCTGAGAACGGCTTCTTTGGGGTTGCCCCTGGTACCTCTGCCACC
  ACCAATCCCAACGCCATGGCTACAATCCAGAGTAACACTATTTTTACCAATGTGGCTGAG
  ACCAGTGATGGTGGCGTGTACTGGGAGGGCATTGACCAGCCTCTTCCACCTGGTGTTACT
  GTGACCTCCTGGCTGGGCAAACCCTGGAAACCTGGTGACAAGGAGCCCTGTGCACATCCC
  AACTCTCGATTTTGTGCCCCGGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAGGCC
  CCAGAGGGTGTCCCCATTGACGCCATCATCTTTGGTGGCCGCAGACCCAAAGGGGTACCC
  CTGGTATACGAGGCCTTCAACTGGCGTCATGGGGTGTTTGTGGGCAGCGCCATGCGCTCT
  GAGTCCACTGCTGCAGCAGAACACAAAGGGAAGATCATCATGCACGACCCATTTGCCATG
  CGGCCCTTTTTTGGCTACAACTTCGGGCACTACCTGGAACACTGGCTGAGCATGGAAGGG
  CGCAAGGGGGCCCAGCTGCCCCGTATCTTCCATGTCAACTGGTTCCGGCGTGACGAGGCA
  GGGCACTTCCTGTGGCCAGGCTTTGGGGAGAATGCTCGGGTGCTAGACTGGATCTGCCGG
  CGGTTAGAGGGGGAGGACAGTGCCCGAGAGACACCCATTGGGCTGGTGCCAAAGGAAGGA
  GCCTTGGATCTCAGCGGCCTCAGAGCTATAGACACCACTCAGCTGTTCTCCCTCCCCAAG
  GACTTCTGGGAACAGGAGGTTCGTGACATTCGGAGCTACCTGACAGAGCAGGTCAACCAG
  GATCTGCCCAAAGAGGTGTTGGCTGAGCTTGAGGCCCTGGAGAGACGTGTGCACAAAATG
  TGA
  
  Clone variation with respect to NM_004563.2
  362 a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004563 unedited
  CCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGGCTCCGCT
  CGGTTCCTGGCCACCCCGCAGCCCCTGCCCAGGTGCCATGGCCGCATTGTACCGCCCTGG
  CCTGCGGCTTAACTGGCATGGGCTGAGCCCCTTGGGCTGGCCATCATGCCGTAGCATCCA
  GACCCTGCGAGTGCTTAGTGGAGATCTGGGCCAGCTTCCCACTGGCATTCGAGATTTTGT
  AGAGCACAGTGCCCGCCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGAACTGAGGC
  TGAGAATACTGCCACACTGACCCTGCTGGAGCAGCAGGGCCTCATCCGAAAGCTCCCCAA
  GTACAATAACTGCTGGCTGGCCCGCACAGACCCCAAGGATGTGGCACGAGTAGAGAGCAA
  GACGGTGATTGTAACTCCTTCTCAGCGGGACACGGTACCACTCCCGCCTGGTGGGGCCCG
  TGGGCAGCTGGGCAACTGGATGTCCCCAGCTGATTTCCAGCGAGCTGTGGATGAGAGGTT
  TCCAGGCTGCATGCAGGGCCGCACCATGTATGTGCTTCCATTCAGCATGGGTCCTGTGGG
  CTCCCCGCTGTCCCGCATCGGGGTGCAGCTCACTGACTCAGCCTATGTGGTGGCAAGCAT
  GCGTATTATGACCCGACTGGGGACACCTGTGCTTCAGGCCCTGNGAGATGGTGACTNTGT
  CAAGTGTCTGCACTCCGTGGGCCAGCCCCTGACAGGACAAGGNGAGCCAGTGAGCCAGTG
  GCCGTGCCACCCAGAGAAACCCTGATTGGTCACGTGCCCGACCAGCGGGAGATCATCTCC
  CTCGGCAGCGGCTATGGTGGGCACTCCCTGCTGGGNCAGAAGTGCTTTGCCCTACGCATC
  GNCTCTCGGCTGCCCCGGNATGAGGNNNCTGCTGCANACACATGCTGATCCTGGCATCAC
  AGACTGCAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004563 unedited
  GACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTGTAAAATAGATAAG
  CATCTCTTATTATTGGACAGTCTTTGTGGGAAGGTCTATGGCACATTGAAGATGTTAGTT
  AATATCAAATTGCTCATATTTTCTGCAAGGTGCCTTCCCTATTCCCAGATGAGGGTGCTA
  TGTCCTCTTGCTAGACTAGGGCCTCAGGTCACATTTTGTGCACACGTCTCTCCAGGGCCT
  CAAGCTCAGCCAACACCTCTTTGGGCAGATCCTGGTTGACCTGCTCTGTCAGGTAGCTCC
  GAATGTCACGAACCTCCTGTTCCCAGAAGTCCTTGGGGAGGGAGAACAGCTGAGTGGTGT
  CTATAGCTCTGAGGCCGCTGAGATCCAAGGCTCCTTCCTTTGGCACCAGCCCAATGGGTG
  TCTCTCGGGCACTGTCCTCCCCCTCTAACCGCCGGCAGATCCAGTCTAGCACCCGAGCAT
  TCTCCCCAAAGCCTGGCCACAGGAAGTGCCCTGCCTCGTCACGCCGGAACCAGTTGACAT
  GGAAGATACGGGGCAGCTGGGCCCCCTTGCGCCCTTCCATGCTCAGCCAGTGTTCCAGGT
  AGTGCCCGAAGTTGTAGCCAAAAAAGGGCCGCATGGCAAATGGGTCGTGCATGATGATCT
  TCCCTTTGTGTTCTGCTGCAGCAGTGGACTCAGAGCGCATGGCGCTGCCCACAAACACCC
  CATGACGCCAGTTGAAGGCCTCGTATACCAGGGGTACCCCCTTTGGGTCTGCGGCCACCA
  AGATGATGGCGTCAATGGGGACACCCCCTCTGGGCCTNCCCAGCCTGGTCCATGATGGNG
  GCACTGGCGAGCCCGNGCACAAATCGAGAGTTGGATGTGACAGGGCTCCTTGTACCAGTT
  NCCAGGTTGCCCAGCAGNAGTACAGTACACCAGNTGGAAAGCTGTCATGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004563
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004563.2, NP_004554.2
• RefSeq Size: 2221 bp
• RefSeq ORF: 1923 bp
• Locus ID: 5106
• UniProt ID: Q16822
• Cytogenetics: 14q11.2-q12
• Domains: PEPCK
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adipocytokine signaling pathway, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism
• Gene Summary: This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).