YB1 (YBX1) (NM_004559) Human Untagged Clone

CAT#: SC117278

YBX1 (untagged)-Human Y box binding protein 1 (YBX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YB1
• Synonyms: BP-8; CBF-A; CSDA2; CSDB; DBPB; EFI-A; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117278 sequence for NM_004559 edited (data generated by NextGen Sequencing)
  ATGAGCAGCGAGGCCGAGACCCAGCAGCCGCCCGCCGCCCCCCCCGCCGCCCCCGCCCTC
  AGCGCCGCCGACACCAAGCCCGGCACTACGGGCAGCGGCGCAGGGAGCGGTGGCCCGGGC
  GGCCTCACATCGGCGGCGCCTGCCGGCGGGGACAAGAAGGTCATCGCAACGAAGGTTTTG
  GGAACAGTAAAATGGTTCAATGTAAGGAACGGATATGGTTTCATCAACAGGAATGACACC
  AAGGAAGATGTATTTGTACACCAGACTGCCATAAAGAAGAATAACCCCAGGAAGTACCTT
  CGCAGTGTAGGAGATGGAGAGACTGTGGAGTTTGATGTTGTTGAAGGAGAAAAGGGTGCG
  GAGGCAGCAAATGTTACAGGTCCTGGTGGTGTTCCAGTTCAAGGCAGTAAATATGCAGCA
  GACCGTAACCATTATAGACGCTATCCACGTCGTAGGGGTCCTCCACGCAATTACCAGCAA
  AATTACCAGAATAGTGAGAGTGGGGAAAAGAACGAGGGATCGGAGAGTGCTCCCGAAGGC
  CAGGCCCAACAACGCCGGCCCTACCGCAGGCGAAGGTTCCCACCTTACTACATGCGGAGA
  CCCTATGGGCGTCGACCACAGTATTCCAACCCTCCTGTGCAGGGAGAAGTGATGGAGGGT
  GCTGACAACCAGGGTGCAGGAGAACAAGGTAGACCAGTGAGGCAGAATATGTATCGGGGA
  TATAGACCACGATTCCGCAGGGGCCCTCCTCGCCAAAGACAGCCTAGAGAGGACGGCAAT
  GAAGAAGATAAAGAAAATCAAGGAGATGAGACCCAAGGTCAGCAGCCACCTCAACGTCGG
  TACCGCCGCAACTTCAATTACCGACGCAGACGCCCAGAAAACCCTAAACCACAAGATGGC
  AAAGAGACAAAAGCAGCCGATCCACCAGCTGAGAATTCGTCCGCTCCCGAGGCTGAGCAG
  GGCGGGGCTGAGTAA
  
  Clone variation with respect to NM_004559.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004559 unedited
  TGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGGGAGCGGAGAGCGGACCC
  CAGAGAGCCCTGAGCAGCCCCACCGCCGCCGCCGGCCTAGTTACCATCACACCCCGGGAG
  GAGCCGCAGCTGCCGCAGCCGGCCCCAGTCACCATCACCGCAACCATGAGCAGCGAGGCC
  GAGACCCAGCAGCCGCCCGCCGCCCCCCCCGCCGCCCCCGCCCTCAGCGCCGCCGACACC
  AAGCCCGGCACTACGGGCAGCGGCGCAGGGAGCGGTGGCCCGGGCGGCCTCACATCGGCG
  GCGCCTGCCGGCGGGGACAAGAAGGTCATCGCAACGAAGGTTTTGGGAACAGTAAAATGG
  TTCAATGTAAGGAACGGATATGGTTTCATCAACAGGAATGACACCAAGGAAGATGTATTT
  GTACACCAGACTGCCATAAAGAAGAATAACCCCAGGAAGTACCTTCGCAGTGTAGGAGAT
  GGAGAGACTGTGGAGTTTGATGTTGTTGAAGGAGAAAAGGGTGCGGAGGCAGCAAATGTT
  ACAGGTCCTGGTGGTGTTCCAGTTCAAGGCAGTAAATATGCAGCAGACCGTAACCATTAT
  AGACGCTATCCACGTCGTNAGGGGGTCCTCCACGCAATTACCAGCAAAATTACCAGAATA
  GTGGAGAGTGGGGGAAAAGAACGAGGGATCGGNAGAGTGCTNNCCGAAGGCCAGGCCCAA
  CAACGCCGGCCCCTACCGCAGGCGAAAGGTCCCACCTTACTACATGCGGAGACCCTAATG
  GGCGTCGACCACAGTATTCCAACCCTCTGTGCAGGGAGAGGATTGGGAGGGTGCTGACAC
  CCAGGTGCAGGAGAACAAGGTAGACCAGTGAGCAGATATGTATTCGGGGAATAGACACGA
  TNCCGCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004559 unedited
  CACAACACAAAACCCCACAAAACAACCCCCCCCCCACAAACCCATTGCTTTTTACTCTGC
  NCNCGACGGCCCGCTTTTTNANGATCGAGTTTTTTTTTTTTTTTTTTTAATTATTTAAGA
  CCTTTATTAACAGGTGCTTGCAGTTTGTTGACTTTTTTCGAAAAAATCAAGTTGTAAACT
  TTTATTACAAATTAAAAATGAAGTTCTTAAAAATCTCAACTTGACCAGATATGAAACAAT
  TTAAAAACCTTTAAAGGCGTATTGAGAAAAACCAGGCTTTTTTAAAAAACACGTTTGTTA
  TTACCAAAAAGAGACGTCTTTAGGTAAAAATAATAAAAACCCCATGCTGCATAGATACTG
  CAGATAGTTCTATTTATCTGGTCAACGGGCAAAAAGCAAGCACTTTAGGTCTTCAGCTCC
  AATCTTTTGTTCATTTCTTATTGCTGGAATTTCATATTTCTTCTTGTTGGATGACTAAAC
  CGGATGATGGTAGAGATGGTAAGCCGGCATTTACTCAGCCCCGCCCTGCTCAGCCTCGGG
  AGCGGACGAATTCTCAGCTGGTGGATCGGCTGCCTTTGTCTCTTTGCCATCTTGTGGTTT
  AGGGTCTTCTGGGCGTCTGCGTCGGTAATTGAAGTTGCGGCGGTACCGACGTTGAGGTGG
  CTGTTGACCTTGGGCCTCATCTCCTTGATTTTCTTTATCTTCTTAATTGCCCGCCTCTCT
  AGGCTTGCTTTGGCGAGGAGGGCCCCTGGGGAACCGGGGCTATACCCCCCAACCTATCCT
  GCCTCCTGGGTTACCTTGCTCCCCTGCACCCCGGTTGGCAGCACCCTCCATAATTTTTCC
  CTGCCCAGGAGGGTTTGGAATCCTGTGCCCCACCCCCTAAGGTCTTCCCATGGCACAAGG
  GGGGAACCCTTCCCCTGCGGGAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004559
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004559.2, NP_004550.2
• RefSeq Size: 1554 bp
• RefSeq ORF: 975 bp
• Locus ID: 4904
• UniProt ID: P67809
• Cytogenetics: 1p34.2
• Domains: CSD
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.