TRAF5 (NM_004619) Human Untagged Clone

CAT#: SC117268

TRAF5 (untagged)-Human TNF receptor-associated factor 5 (TRAF5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAF5
• Synonyms: MGC:39780; RNF84
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004619 edited
  GAATTCGGCACGAGCGCCGGCCTCAGCCAGGAGCAGCAGCCGCGCCTGCAGACCGGCCTC
  GCGGAGCCCGCGCGCCGAGCCCCACAATGGCTTATTCAGAAGAGCATAAAGGTATGCCCT
  GTGGTTTCATCCGCCAGAATTCCGGCAACTCCATTTCCTTGGACTTTGAGCCCAGTATAG
  AGTACCAGTTTGTGGAGCGGTTGGAAGAGCGCTACAAATGTGCCTTCTGCCACTCGGTGC
  TTCACAACCCCCACCAGACAGGATGTGGGCACCGCTTCTGCCAGCACTGCATCCTGTCCC
  TGAGAGAATTAAACACAGTGCCAATCTGCCCTGTAGATAAAGAGGTCATCAAATCTCAGG
  AGGTTTTTAAAGACAATTGTTGCAAAAGAGAAGTCCTCAACTTATATGTATATTGCAGCA
  ATGCTCCTGGATGTAATGCCAAGGTTATTCTGGGCCGGTACCAGGATCACCTTCAGCAGT
  GCTTATTTCAACCTGTGCAGTGTTCTAATGAGAAGTGCCGGGAGCCAGTCCTACGGAAAG
  ACCTGAAAGAGCATTTGAGTGCATCCTGTCAGTTTCGAAAGGAAAAATGCCTTTATTGCA
  AAAAGGATGTGGTAGTCATCAATCTACAGAATCATGAGGAAAACTTGTGTCCTGAATACC
  CAGTATTTTGTCCCAACAATTGTGCGAAGATTATTCTAAAAACTGAGGTAGATGAACACC
  TGGCTGTATGTCCTGAAGCTGAGCAAGACTGTCCTTTTAAGCACTATGGCTGTGCTGTAA
  CGGATAAACGGAGGAACCTGCAGCAACATGAGCATTCAGCCTTACGGGAGCACATGCGTT
  TGGTTTTAGAAAAGAATGTCCAATTAGAAGAACAGATTTCTGACTTACACAAGAGCCTAG
  AACAGAAAGAAAGTAAAATCCAGCAGCTAGCAGAAACTATAAAGAAACTTGAAAAGGAGT
  TCAAGCAGTTTGCACAGTTGTTTGGCAAAAATGGAAGCTTCCTCCCAAACATCCAGGTTT
  TTGCCAGTCACATTGACAAGTCAGCTTGGCTAGAAGCTCAAGTGCATCAATTATTACAAA
  TGGTTAACCAGCAACAAAATAAATTTGACCTGAGACCTTTGATGGAAGCAGTTGATACAG
  TGAAACAGAAAATTACCCTGCTAGAAAACAATGATCAAAGATTAGCCGTTTTAGAAGAGG
  AAACTAACAAACATGATACCCACATTAATATTCATAAAGCACAGCTGAGTAAAAATGAAG
  AGCGATTTAAACTGCTGGAGGGTACTTGCTATAATGGAAAGCTCATTTGGAAGGTGACAG
  ATTACAAGATGAAGAAGAGAGAGGCGGTGGATGGGCACACAGTGTCCATCTTCAGCCAGT
  CCTTCTACACCAGCCGCTGTGGCTACCGGCTCTGTGCTAGAGCATACCTGAATGGGGATG
  GGTCAGGGAGGGGGTCACACCTGTCCCTATACTTTGTGGTCATGCGAGGAGAGTTTGACT
  CACTGTTGCAGTGGCCATTCAGGCAGAGGGTGACCCTGATGCTTCTGGACCAGAGTGGCA
  AAAAGAACATTATGGAGACCTTCAAACCTGACCCCAATAGCAGCAGCTTTAAAAGACCTG
  ATGGGGAGATGAACATTGCATCTGGCTGTCCCCGCTTTGTGGCTCATTCTGTTTTGGAGA
  ATGCCAAGAACGCCTACATTAAAGATGACACTCTGTTCTTGAAAGTGGCCGTGGACTTAA
  CTGACCTGGAGGATCTCTAGTCACTGTTATGGGGTGATAAGAGGACTTCTTGGGGCCAGA
  ACTGTGGAGGAGAGCACATTTGATTATCATATTGACCTGGATTTAGACTCAAAGCACATT
  TGTATTTGCCTTTTTCCTTAACGTTTGAAGTCAGTTTAAAACTTCTGAAGTGCTGTCTTT
  TTACATTTTACTCTGTCCCAGTTTGAAACTTAAAACTCTTAGAATATTCTCTTATTATTT
  ATATTTTTATATTTCTTGAAAGATGGTAAGTTTCTTGAAGTTXXXXXXXXXXXXXXXXXX
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004619 unedited
  GTTGTCAGAATTTTGGTATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGCGCC
  GGCCTCAGCCAGGAGCAGCAGCCGCGCCTGCAGACCGGCCTCGCGGAGCCCGCGCGCCGA
  GCCCCACAATGGCTTATTCAGAAGAGCATAAAGGTATGCCCTGTGGTTTCATCCGCCAGA
  ATTCCGGCAACTCCATTTCCTTGGACTTTGAGCCCAGTATAGAGTACCAGTTTGTGGAGC
  GGTTGGAAGAGCGCTACAAATGTGCCTTCTGCCACTCGGTGCTTCACAACCCCCACCAGA
  CAGGATGTGGGCACCGCTTCTGCCAGCACTGCATCCTGTCCCTGAGAGAATTAAACACAG
  TGCCAATCTGCCCTGTAGATAAAGAGGTCATCAAATCTCAGGAGGTTTTTAAAGACAATT
  GTTGCAAAAGAGAAGTCCTCAACTTATATGTATATTGCAGCAATGCTCCTGGATGTAATG
  CCAAGGTTATTCTGGGCCGGTACCAGGATCACCTTCAGCAGTGCTTATTTCAACCTGTGC
  AGTGTTCTAATGAGAAGTGCCGGGAGCCAGTCCTACGGAAAGACCTGAAAGAGCATTTGA
  GTGCATCCTGTCAGTTTCGAAAGGAAAAATGCCTTTATTGCAAAAAGGATGTGGTAGTCA
  TCAATCTACAGAATCATGAGGAAAACTTGTGTCCTGAATACCCAGTATTTTGTCCCAACA
  ATTGTGCGAAGATTATTCTAAAAACTGAGGTAGATGAACACCTGGCTGTATGTCCTGAAG
  CTGAGCAAGACTGTCCTTTTAAGCACTATGGCTGTGCTGTAACGGATAAACGGAGGAACC
  TGCAGCACATGAGCATTCAGCCTTACGGGAGCACATGCGTTTGGTTNTAGAANAGATGTC
  CATTAGAAGACAGATTCTGACTACACAGAACTAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004619 unedited
  GTAGTGCTTTCTTCTTTTATATTCCTTGTTCTGGGATCACTTAATCACTATTTCGATGAT
  GAAGGAACTCTGAACTCCTAAGCAAACCATGCCATGACATACTTTATATATATGCACAAA
  AGTTGGATAAAACACAAAACCAGCATTTTGACAAATTCACTTTAAAATGTTTTCCACATC
  AACTACTTAAAAGAAGTTACAGCAATATGATCTTCCACTGCTCAGGAAGACAGAGTGAAC
  TTGGATATGATGAATATTAAAAACAAACATACAGAGCACCTGGGTCTGGACTTGTGGGCT
  AAAGAAATGCTTCCTGAATTTGTCCGAGGTGATGTGTTGAGCAACCGTGCCACAGTTCAC
  CAGCAGTGACACTCTAGTTCTTCAGTATATTCCTGTTGTGAACCTATTTCTTTGAATTTA
  ATCATCTGGGATGATTGCTGCAGTGTGAGTCTTTAGCATTTTGAAAATCCCACAGAAGAG
  ATTAAGAAGGTTCCTCTTCCCAGAAGACACTCATATAAAGCATGCTTCTATAGGGTTAAC
  TGTTCAGAAATTGTAAGGTGCATGGTCATCACCAGGTAAGCAGCTCTACAGGGGCCCATG
  GCCCATACTCATCCTTGCGTGCCCAGGGGAGCCAGCGTGCATATGAGCAGTAGGGAAGTG
  GCATAACAACGCAGAAGGGCTCAGGACAACTTGGCATTTTTGTTGTTGTTAAACTCAGGC
  TTATCAAGAGTCATAAGGACTCATCTCAGTTTGTATATACACCTTCAGACCCATACAGAT
  AGATGCCTAAGAATATGATCATTTCTGGTTTTGACAGAGTACTCAAGATTCCTGACAGGA
  AACCATACCAAGTTATATTAAATGCCATTTGCATAGCAGGCTACCAATTCAATAGTTGTC
  ACAGGCTTGACTCGGCGGGTATGCCTGTCATTTCACACCTTTGGGAGGCCGAGGCGGCAA
  ATACTCGAGGCCCGGATTGAGAACAGTTGGCAACATGCAAAATTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004619
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004619.2, NP_004610.1
• RefSeq Size: 4132 bp
• RefSeq ORF: 1674 bp
• Locus ID: 7188
• UniProt ID: O00463
• Cytogenetics: 1q32.3
• Domains: zf-TRAF, RING, MATH
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Small cell lung cancer
• Gene Summary: The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 1, 2, and 3 all encode the same isoform (b).