EDG4 (LPAR2) (NM_004720) Human Untagged Clone

CAT#: SC117226

LPAR2 (untagged)-Human lysophosphatidic acid receptor 2 (LPAR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDG4
• Synonyms: EDG-4; EDG4; LPA-2; LPA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004720 edited
  GAATTCGGCACGAGGGCAACCCGCACAGGGCGCACCCGGACGCTCTACCGCTCCCGCCGC
  AGTCGCCGGGCCATGGGCCTCGAGCCCGCCCCGAACCCCCGCGAGCCCGCCTTGTCTGCG
  GCGTGACTGGAGGCCCAGATGGTCATCATGGGCCAGTGCTACTACAACGAGACCATCGGC
  TTCTTCTATAACAACAGTGGCAAAGAGCTCAGCTCCCACTGGCGGCCCAAGGATGTGGTC
  GTGGTGGCACTGGGGCTGACCGTCAGCGTGCTGGTGCTGCTGACCAATCTGCTGGTCATA
  GCAGCCATCGCCTCCAACCGCCGCTTCCACCAGCCCATCTACTACCTGCTCGGCAATCTG
  GCCGCGGCTGACCTCTTCGCGGGCGTGGCCTACCTCTTCCTCATGTTCCACACTGGTCCC
  CGCACAGCCCGACTTTCACTTGAGGGCTGGTTCCTGCGGCAGGGCTTGCTGGACACAAGC
  CTCACTGCGTCGGTGGCCACACTGCTGGCCATCGCCGTGGAGCGGCACCGCAGTGTGATG
  GCCGTGCAGCTGCACAGCCGCCTGCCCCGTGGCCGCGTGGTCATGCTCATTGTGGGCGTG
  TGGGTGGCTGCCCTGGGCCTGGGGCTGCTGCCTGCCCACTCCTGGCACTGCCTCTGTGCC
  CTGGACCGCTGCTCACGCATGGCACCCCTGCTCAGCCGCTCCTATTTGGCCGTCTGGGCT
  CTGTCGAGCCTGCTTGTCTTCCTGCTCATGGTGGCTGTGTACACCCGCATTTTCTTCTAC
  GTGCGGCGGCGAGTGCAGCGCATGGCAGAGCATGTCAGCTGCCACCCCCGCTACCGAGAG
  ACCACGCTCAGCCTGGTCAAGACTGTTGTCATCATCCTGGGGGCGTTCGTGGTCTGCTGG
  ACACCAGGCCAGGTGGTACTGCTCCTGGATGGTTTAGGCTGTGAGTCCTGCAATGTCCTG
  GCTGTAGAAAAGTACTTCCTACTGTTGGCCGAGGCCAACTCACTGGTCAATGCTGCTGTG
  TACTCTTGCCGAGATGCTGAGATGCGCCGCACCTTCCGCCGCCTTCTCTGCTGCGCGTGC
  CTCCGCCAGTCCACCCGCGAGTCTGTCCACTATACATCCTCTGCCCAGGGAGGTGCCAGC
  ACTCGCATCATGCTTCCCGAGAACGGCCACCCACTGATGGACTCCACCCTTTAGCTACCT
  TGAACTTCAGCGGTACGCGGCAAGCAACAAATCCACAGCCCCTGATGACTTGTGGGTGCT
  CCTGGCTCAACCCAACCAACAGGACTGACTGACTGGCAGGACAAGGTCTGGCATGGCACA
  GCACCACTGCCAGGCCTCCCCAGGCACACCACTCTGCCCAGGGAATGGGGGCTTTGGGTC
  ATCTCCCACTGCCTGGGGGAGTCAGATGGGGTGCAGGAATCTGGCTCTTCAGCCATCTCA
  GGTTTAGGGGGTTTGTAACAGACATTATTCTGTTTTCACTGCGTATCCTTGGTAAGCCCT
  GTGGACTGGTTCCTGCTGTGTGATGCTGAGGGTTTTAAGGTGGGGAGAGATAAGGGCTCT
  CTCGGGCCATGCTACCCGGTATGACTGGGTAATGAGGACAGACTGTGGACACCCCATCTA
  CCTGAGTCTGATTCTTTAGCAGCAGAGACTGAGGGGTGCAGAGTGTGAGCTGGGAAAGGT
  TTGTGGCTCCTTGCAGCCTCCAGGGACTGGCCTGTCCCCGATAGAATTGAAGCAGTCCAC
  GGGGAGGGGATGATACAAGGAGTAAACCTTTCTTTACACTCAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004720 unedited
  NGGTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCAACCCGC
  ACAGGGCGCACCCGGACGCTCTACCGCTCCCGCCGCAGTCGCCGGGCCATGGGCCTCGAG
  CCCGCCCCGAACCCCCGCGAGCCCGCCTTGTCTGCGGCGTGACTGGAGGCCCAGATGGTC
  ATCATGGGCCAGTGCTACTACAACGAGACCATCGGCTTCTTCTATAACAACAGTGGCAAA
  GAGCTCAGCTCCCACTGGCGGCCCAAGGATGTGGTCGTGGTGGCACTGGGGCTGACCGTC
  AGCGTGCTGGTGCTGCTGACCAATCTGCTGGTCATAGCAGCCATCGCCTCCAACCGCCGC
  TTCCACCAGCCCATCTACTACCTGCTCGGCAATCTGGCCGCGGCTGACCTCTTCGCGGGC
  GTGGCCTACCTCTTCCTCATGTTCCACACTGGTCCCCGCACAGCCCGACTTTCACTTGAG
  GGCTGGTTCCTGCGGCAGGGCTTGCTGGACACAAGCCTCACTGCGTCGGTGGCCACACTG
  CTGGCCATCGCCGTGGAGCGGCACCGCAGTGTGATGGCCGTGCAGCTGCACAGCCGCCTG
  CCCCGTGGCCGCGTGGTCATGCTCATTGTGGGCGTGTGGGTGGCTGCCCTGNGCCTGGGG
  CTGCTGCCTGCCCACTCCTGGCACTGCCTCTGTGCCCTGGACCGCTGCTCACGCATGGCA
  CCCCTGCTCAGCCGCTCCTATTTGGCCGTCTGGGCTCTGTCGAGCCTGCTTGTCTTCCTG
  CTCATGGGGNCTGTGTACACCCGCATTNTCTTTCTACGTGCGCGGCGAGTGCAGCGCATG
  GCANAGCATGTCAGCTGCCACCCCCGCTACCGAGAGACCACGCTCAGCCTGGTCAGAACT
  GNTGTCATCATCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004720 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGGGGAAAG
  AAAGGGTTACTCCTTGGATCATCCCCTCCCCGTGGACTGCTTCAATTCTATCGGGGACAG
  GCCAGTCCCTGGAGGCTGCAAGGAGCCACAAACCTTTCCCAGCTCACACTCTGCACCCCT
  CAGTCTCTGCTGCTAAAGAATCAGACTCAGGTAGATGGGGTGTCCACAGTCTGTCCTCAT
  TACCCAGTCATACCGGGTAGCATGGCCCGAGAGAGCCCTTATCTCTCCCCACCTTAAAAC
  CCTCAGCATCACACAGCAGGAACCAGTCCACAGGGCTTACCAAGGATACGCAGTGAAAAC
  AGAATAATGTCTGTTACAAACCCCCTAAACCTGAGATGGCTGAAGAGCCAGATTCCTGCA
  CCCCATCTGACTCCCCCAGGCAGTGGGAGATGACCCAAAGCCCCCATTCCCTGGGCAGAG
  TGGTGTGCCTGGGGAGGCCTGGCAGTGGTGCTGTGCCATGCCAGACCTTGTCCTGCCAGT
  CAGTCAGTCCTGTTGGTTGGGTTGAGCCAGGAGCACCCACAAGTCATCAGGGGCTGTGGA
  TTTGTTGCTTGCCGCGTACCGCTGAAGTTCAAGGTAGCTAAAGGGTGGAGTCCATCAGTG
  GGTGGCCGTTCTCGGGAAGCATGATGCGAGTGCTGGCACCTCCCTGGGCAGAGGATGTAT
  AGTGGACAGACTCGCGGGTGGACTGGNCGGAGCCGCGCANCANAGAAGCCGCGAAGGTGC
  GGCGCATCTAGCATCTCGCAAGAGTCACAGCAGCTTGACAGTGNAGTGGCCTCCGCCAAC
  AGTAGAGTACTTTTCTCAGCCAGACATGCAGACTCCAGCCCTAACTCCAGGACAGTACAC
  CTGCCTGGGTCCACAGACACGACGCCCCAGAGAGACACATTTGACAGCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004720
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004720.4, NP_004711.2
• RefSeq Size: 1868 bp
• RefSeq ORF: 1056 bp
• Locus ID: 9170
• UniProt ID: Q9HBW0
• Cytogenetics: 19p13.11
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]