LATS1 (NM_004690) Human Untagged Clone

CAT#: SC117200

LATS1 (untagged)-Human LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LATS1
• Synonyms: WARTS; wts
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004690 edited
  ATGAAGAGGAGTGAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCC
  AGTAACTATACTGTCAGTAGCCGGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAAT
  TTATCTAAACCATCTGATGCTGCTAAGGCTGAGCATAACATGAGTAAAATGTCAACCGAA
  GATCCTCGACAAGTCAGAAATCCACCCAAATTTGGGACGCATCATAAAGCCTTGCAGGAA
  ATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATTCTTCTCGGAGTACTTCAGAA
  GTTAATCCACAAATGCTTCAAGACTTGCAAGCTGCTGGATTTGATGAGGATATGGTTATA
  CAAGCTCTTCAGAAAACTAACAACAGAAGTATAGAAGCAGCAATTGAATTCATTAGTAAA
  ATGAGTTACCAAGATCCTCGACGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATTAAT
  GCCAGCATGAAACCAGGGAATGTGCAGCAATCAGTTAACCGCAAACAGAGCTGGAAAGGT
  TCTAAAGAATCCTTAGTTCCTCAGAGGCATGGCCCGCCACTAGGAGAAAGTGTGGCCTAT
  CATTCTGAGAGTCCCAACTCACAGACAGATGTAGGAAGACCTTTGTCTGGATCTGGTATA
  TCAGCATTTGTTCAAGCTCACCCTAGCAACGGACAGAGAGTGAACCCCCCACCACCACCT
  CAAGTAAGGAGTGTTACTCCTCCACCACCTCCAAGAGGCCAGACTCCCCCTCCAAGAGGT
  ACAACTCCACCTCCCCCTTCATGGGAACCAAACTCTCAAACAAAGCGCTATTCTGGAAAC
  ATGGAATACGTAATCTCCCGAATCTCTCCTGTCCCACCTGGGGCATGGCAAGAGGGCTAT
  CCTCCACCACCTCTCAACACTTCCCCCATGAATCCTCCTAATCAAGGACAGAGAGGCATT
  AGTTCTGTTCCTGTTGGCAGACAACCAATCATCATGCAGAGTTCTAGCAAATTTAACTTT
  CCATCAGGGAGACCTGGAATGCAGAATGGTACTGGACAAACTGATTTCATGATACACCAA
  AATGTTGTCCCTGCTGGCACTGTGAATCGGCAGCCACCACCTCCATATCCTCTGACAGCA
  GCTAATGGACAAAGCCCTTCTGCTTTACAAACAGGGGGATCTGCTGCTCCTTCGTCATAT
  ACAAATGGAAGTATTCCTCAGTCTATGATGGTGCCAAACAGAAATAGTCATAACATGGAA
  CTATATAACATTAGTGTACCTGGACTGCAAACAAATTGGCCTCAGTCATCTTCTGCTCCA
  GCCCAGTCATCCCCGAGCAGTGGGCATGAAATCCCTACATGGCAACCTAACATACCAGTG
  AGGTCAAATTCTTTTAATAACCCATTAGGAAATAGAGCAAGTCACTCTGCTAATTCTCAG
  CCTTCCGCTACAACAGTCACTGCAATTACACCAGCTCCTATTCAACAGCCTGTGAAAAGT
  ATGCGTGTATTAAAACCAGAGCTACAGACTGCTTTAGCACCTACACACCCTTCTTGGATA
  CCACAGCCAATTCAAACTGTTCAACCCAGTCCTTTTCCTGAGGGAACCGCTTCAAATGTG
  ACTGTGATGCCACCTGTTGCTGAAGCTCCAAACTATCAAGGACCACCACCACCCTACCCA
  AAACATCTGCTGCACCAAAACCCATCTGTTCCTCCATACGAGTCAATCAGTAAGCCTAGC
  AAAGAGGATCAGCCAAGCTTGCCCAAGGAAGATGAGAGTGAAAAGAGTTATGAAAATGTT
  GATAGTGGGGATAAAGAAAAGAAACAGATTACAACTTCACCTATTACTGTTAGGAAAAAC
  AAGAAAGATGAAGAGCGAAGGGAATCTCGTATTCAAAGTTATTCTCCTCAAGCATTTAAA
  TTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTCATCAGCAGCGTCTACATCGT
  AAAAAACAATTAGAGAATGAAATGATGCGGCTGATTTAAACTTTTATGGTGTTGGAAGCT
  TTGCTGACTTCTGTAGTCTCCATTACTGTGTAGGTTGGATTATCTCAAGATGCCCAGGAT
  CAAATGAGAAAGATGCTTTGCCAAAAAGAATCTAATTACATCCGTCTTAAAAGGGCTAAA
  ATGGACAAGTCTATGTTTGTGAAGATAAAGACACTAGGAATAGGAGCATTTGGTGAAGTC
  TGTCTAGCAAGAAAAGTAGATACTAAGGCTTTGTATGCAACAAAAACTCTTCGAAAGAAA
  GATGTTCTTCTTCGAAATCAAGTCGCTCATGTTAAGGCTGAGAGAGATATCCTGGCTGAA
  GCTGACAATGAATGGGTAGTTCGTCTATATTATTCATTCCAAGATAAGGACAATTTATAC
  TTTGTAATGGACTACATTCCTGGGGGTGATATGATGAGCCTATTAATTAGAATGGGCATC
  TTTCCAGAAAGTCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGAAAGTGTT
  CATAAAATGGGTTTTATTCATAGAGATATTAAACCTGATAATATTTTGATTGATCGTGAT
  GGTCATATTAAATTGACTGACTTTGGCCTCTGCACTGGCTTCAGATGGACACACGATTCT
  AAGTACTATCAGAGTGGTGACCATCCACGGCAAGATAGCATGGATTTCAGTAATGAATGG
  GGGGATCCCTCAAGCTGTCGATGTGGAGACAGACTGAAGCCATTAGAGCGGAGAGCTGCA
  CGCCAGCACCAGCGATGTCTAGCACATTCTTTGGTTGGGACTCCCAATTATATTGCACCT
  GAAGTGTTGCTACGAACAGGATACACACAGTTGTGTGATTGGTGGAGTGTTGGTGTTATT
  CTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACACCATTAGAAACACAA
  ATGAAGGTTATCAACTGGCAAACATCTCTTCACATTCCACCACAAGCTAAACTCAGTCCT
  GAAGCTTCTGATCTTATTATTAAACTTTGCCGAGGACCCGAAGATCGCTTAGGCAAGAAT
  GGTGCTGATGAAATAAAAGCTCATCCATTTTTTAAAACAATTGATTTCTCCAGTGACCTG
  AGACAGCAGTCTGCTTCATACATTCCTAAAATCACACACCCAACAGATACATCAAATTTT
  GATCCTGTTGATCCTGATAAATTATGGAGTGATGATAACGAGGAAGAAAATGTAAATGAC
  ACTCTCAATGGATGGTATAAAAATGGAAAGCATCCTGAACATGCATTCTATGAATTTACC
  TTCCGAAGGTTTTTTGATGACAATGGCTACCCATATAATTATCCGAAGCCTATTGAATAT
  GAATACATTAATTCACAAGGCTCAGAGCAGCAGTCGGATGAAGATGATCAAAACACAGGC
  TCAGAGATTAAAAATCGCGATCTAGTATATGTTTAACACACTAGTAAATAAATGTAATGA
  GGATTTGTAAAAGGGCCTGAAATGCGAGGTGTTTTGAGGTTCTGAGAGTAAAATTATGCA
  AATATGACAGAGCTATATATGTGTGCTCTGTGTACAATATTTTATTTTCCTAAATTATGG
  GAAATCCTTTTAAAATGTTAATTTATTCCAGCCGTTTAAATCAGTATTTAGAAAAAAATT
  GTTATAAGGAAAGTAAATTATGAACTGAATATTATAGTCAGTTCTTGGTACTTAAAGTAC
  TTAAAATAAGTAGTGCTTTGTTTAAAAGGAGAAACCTGGTATCTATTTGTATATATGCTA
  AATAATTTTAAAATACAAGAGTTTTTGAAATTTTTTTGAAAGACAGTTTTAGTTTTATCT
  TGCTTTAACCAAATATGAAACATACCCCCTATTTTACAGAGCTCTTTTTTCCCCTCATAA
  CCTCGTTTTTGGTAGAAAATAAGCTAGAGAAATTAAGCCATCGTGTTGGTGAGTGTTCCT
  AGGCTAATGATAATCTGTATAATTCACATCCTGAAACTAAGGAATACAGGGTTGAAAAAA
  TATTAATATGTTTGTCAGAAGGAAAAATAATGCATTTATCTTCCCCCCCCACCCCCCGCC
  CCATGGAATATTTAATCTATTTAATCTTCTTGCATTTATTTCTCAAGAATTACTGGCTTT
  AAAAGAAGCCAAAGCACTACTAGCTTTTTTTCCATATTGGTATTTTTGATGCTGCTTCCA
  ATTTTAAAAGGGAACAAAGCTGCCATAAATCGAAATGTTCAATACTAAAAGCTAAAATAT
  TTCTCACCATCCTAAGCAGATAATTATTTTAATTTTCATATACTTTTCCTGTATAGTAAC
  TATTTTGATTATATCATCAATGTTACCTGTTTCCTCTTTCAGAACAGTGCTGCATATACA
  GATTGTTATTGGCAAAGGAAAATCTGGCTATCTGGCAATATTTTACCTAAGCGCAGATTA
  ATTGGTGAAAAAATTAACTCTTAAGATGGCCATTAATAATTAGGAAAGTTTACAGAGTGG
  TCTTAGTAGAAAATTCAAGTCCTCCTAATTTATTTAAGGTTCAATAATGCGTTCAACATG
  CCTGTTATGTATAACGCTTAGGTTCTAAGGAAGATTAAGGTTTCATACCAAAATACATGT
  AGCTTATCTTTTAGGAAGGGGAAAAAGGCTCCATTTTGACCATAGTAAAATTTGTGTTGT
  GTTTTATTTCCTTTTCTTAAGCTCCACTGATAAGGGATTGTTTTTATCAAAAGTTACTAT
  TTGTAGATTGGAGGCATAATTTTAGTGATTTTCATACTTTTAGCTTTCTTCGCATAAAAG
  CTAATTGAAACCGTATATGTAGTAAAATTAAAGGCAGAGCTGTTGCAGTTGAATTGGAGA
  GTCAGGGCAAAGAACACTTATTAGCCCACACTTCCCACCTTTCTACAGGTGGTCCTTTCA
  GAGCTCAGCCTGGAAAACCCACTACTGTGTTATCGTGCGTCTTTTGGGGTTAGTGGKTCT
  TTTGAGAATCTGAAGGAAGCTGTGGACTCTTCCTAGAAAAAAAAACCACACATACACATA
  CAATGTTGCATGCAGTTTCAAGGGATTTTGGACATATTGAAACCTATCACAGGCTGTAGG
  TTATGGACCTCTGTGCCATGAGAAAATTGATACATTAAACTAAGAACTTTGTTTTTAACT
  TACCAATCACTACTCAGCACATCTTATATAAGCTGATAATTTGTGATGGAAAAGGTCTGT
  AGCATGTGATATAAGGTGACCTTATGAATGCCTCTCTTGCTGGTACATTAAGTTGTTTTA
  ATATATCATTTGGAGGGGACTGAAATGTTAGGCTCATTACAAGCTTGATACAGAAATATT
  TCTGAAGGATTTCTAATCAGAATTGTAAAACAATGTGCTATCATGAAATCGCAGTCTTCA
  CCTCATGGTTCATGGAACATTTGGTTAGTCCCATAAAATCCTATGCAAAACAAAGTAGTT
  CAAGAATTTTTAGGTGGGTAGTCACATTTATAAGGTATTCCTCTTACTCTTTGGGCTTTT
  TCAGTCTGATTTATTTAAATTTTCATTTAGTTGTTTTACTTTTGGACTAAGGTGCAATAC
  AGTAGAAGATAACTTCGTTACATTTATGTTGTAGGAAAACTAAGGTGCTGTCTCCTCCCC
  CTTCCCTTCCCACAAAATCTGTATTCCCCCTATTGCTGAAATGTAACAGACACTACAAAT
  TTTGTATTCTTTTTTTGTTTTTTGTTTTGAGACAGGGTCTCACTCTGTCACCCAGGCTGG
  AGGGCAGTGGCGCTTCACAGCTCACTGCATCCTCAACCTTGGGGGCTCACGCAGTCCTCC
  CGCCTCAGCCTCCCAAGTAGCTGGGCATGCGCCACCAAGCCCAGCTAATGTTTGTATCTT
  TAGTAGAGATGGGGTTTCGCCATGTTGCCCAGGTTGGTGTGGAATTCCTGGGCTCCAGTT
  ATATGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGACGTGACCCACCGCGCCTGGC
  GCAAATATGTATTCTTTTAAAATTTCCTCTGATACTATAAGCTTTTTGCATTTATCTGAA
  GCAGTATACATGCCTTTGGTATCAGCAATTTTAACAGTTTGGATATACTTATCAGCTATC
  TTATTCCAAAACTACATCTACTTCTTCCAGTATAGAATCTGGTGCTTCCTGACCAAAAAG
  ATGAGAAAAACAATGTTAAAAATATAGATGCTTTCCATTGAAATGGAGTGAAAACATTGG
  TTCTATATGTTTTCTTTTAAAATAATTTTCTTATTAAAAACTTGCTGTCTTTATTATACT
  TACCCTTTTTATGCATATCAATAGTATTTATAAGATGTGTTCTATAATTATGTAATTGTA
  GATACTGTTATGCATTGTCCAGTGACATCATAAGGCAGGCCCTACTGCTGTATCTTTTCT
  ACTTTCTTATTTGTAATAGAAACTATAGAATGTATGACTAAAAAGTCACTTTGAGATTGA
  CTTTTTTAAAAAGTTATTACCTTCTGCTGTTGCAAAGTGCAAAACTGTGAGTGGAATTGT
  TTTATTCTGACTTAATGTGTTAGAAATTAGAGAATACAGTGGGAGGATTTTTAGACATTG
  CTGCTGCTGTTACCCAAGGTATTTTAGATAAAAAATTTTAAATAAACATCCCTTTGGTAT
  TTAAAGTGGAACATTTAGCCTGTTCATTTTAATCTAAAGCAAAAAGTAATTTGGGTCAAA
  ATATTGGTATATTTGTAAAGCGCCTTAATATATCCCTTTGTGGAAGGCACTACACAGTTT
  ACTTTTATATTGTATTGTGTATATAAGTATTTTGTATTAAAATTGAATCAGTGGCAACAT
  TAAAGTTTTATAAAATCATGCTTTGTTAGAAAAAGAATTACAGCTTTGCAATATAACTAA
  TTGTTTTGCATAATTCTGAATGTAATAGATATGAATAATCAGCCTGTGTTTTTAATGAAC
  TTACTTTGTATTTTCCCAATCATTTTCTCTAGTGTAATGTTTGCTGGGATAATAAAAAAA
  ATTCAAATCTTTCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATA
  GCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCGG
  CCCGAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004690 unedited
  GTCAGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACCAGGCGGCGGC
  GGCGGTGGCCTGGTGTTAAGGGGAGAGCCAGGTCCTTACGACCCCTGGGACGGGCCGCGC
  TGGCCCGCGGCAGCCCCCCCGTTCGTCTCCCCGCTCTGCCCCACCAGGGATACTTGGGGT
  TGCTGGGACGGACTCTGGCCGCCTCAGCGTCCGCCCTCAGGCCCGTGGCCGCTGTCCAGG
  AGCTCTGCTCTCCCCTCCAGAGTTAATTATTTATATTGTAAAGAATTTTAACAGTCCTGG
  GGACTTCCTTGAAGCATCATTTTCACTTTTGCTCAGAAGAAAGCTCTGGATCTATCAAAT
  AAAGAAGTCCTTCGTGTGGGCTACATATATAGATGTTTTCATGAAGAGGAGTGAAAAGCC
  AGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATACTGTCAGTAG
  CCGGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGC
  TGCTAAGGCTGAGCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAA
  TCCACCCAAATTTGGGACGCATCATAAAGCCTTGCAGGAAATTCGAAACTCTCTGCTTCC
  ATTTGCAAATGAAACAAATTCTTCTCGGAGTACTTCAGAAGTTAATCCACAAATGCTTCA
  AGACTTGCAAGCTGCTGGATTTGATGAGGATATGGTTATACAAGCTCTTCAGAAACTAAC
  ACAGAATATAGAAGCAGCAATTGAATCATTAGTAAATGAGTACCAAGATCCTCGACGAGA
  GCAGATGCTGCAGCAGCTGCCAGACCTATTATGCCAGCATGAAACCGGGGATGTGCAGNC
  ATCAGTTAACCGCANACAGAGCTGGAAAGGGTCTAAAGATNCCTATTCCTCAGAGCATGG
  NCCGCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004690
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004690.2, NP_004681.1
• RefSeq Size: 4756 bp
• RefSeq ORF: 3393 bp
• Locus ID: 9113
• UniProt ID: O95835
• Cytogenetics: 6q25.1
• Domains: UBA, pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.