ITM2A (NM_004867) Human Untagged Clone

CAT#: SC117063

ITM2A (untagged)-Human integral membrane protein 2A (ITM2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITM2A
• Synonyms: BRICD2A; E25A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117063 sequence for NM_004867 edited (data generated by NextGen Sequencing)
  ATGGTGAAAATCGCCTTCAATACCCCTACCGCCGTGCAAAAGGAGGAGGCGCGGCAAGAC
  GTGGAGGCCCTCCTGAGCCGCACGGTCAGAACTCAGATACTGACCGGCAAGGAGCTCCGA
  GTTGCCACCCAGGAAAAAGAGGGCTCCTCTGGGAGATGTATGCTTACTCTCTTAGGCCTT
  TCATTCATCTTGGCAGGACTTATTGTTGGTGGAGCCTGCATTTACAAGTACTTCATGCCC
  AAGAGCACCATTTACCGTGGAGAGATGTGCTTTTTTGATTCTGAGGATCCTGCAAATTCC
  CTTCGTGGAGGAGAGCCTAACTTCCTGCCTGTGACTGAGGAGGCTGACATTCGTGAGGAT
  GACAACATTGCAATCATTGATGTGCCTGTCCCCAGTTTCTCTGATAGTGACCCTGCAGCA
  ATTATTCATGACTTTGAAAAGGGAATGACTGCTTACCTGGACTTGTTGCTGGGGAACTGC
  TATCTGATGCCCCTCAATACTTCTATTGTTATGCCTCCAAAAAATCTGGTAGAGCTCTTT
  GGCAAACTGGCGAGTGGCAGATATCTGCCTCAAACTTATGTGGTTCGAGAAGACCTAGTT
  GCTGTGGAGGAAATTCGTGATGTTAGTAACCTTGGCATCTTTATTTACCAACTTTGCAAT
  AACAGAAAGTCCTTCCGCCTTCGTCGCAGAGACCTCTTGCTGGGTTTCAACAAACGTGCC
  ATTGATAAATGCTGGAAGATTAGACACTTCCCCAACGAATTTATTGTTGAGACCAAGATC
  TGTCAAGAGTAA
  
  Clone variation with respect to NM_004867.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004867 unedited
  TAGGGCGGCCCGCGATTCGGCACGAGGAAAGTAAGTGCTGTTTGGGCTAACAGGATCTCC
  TCTTGCAGTCTGCAGCCCAGGACGCTGATTCCAGCAGCGCCTTACCGCGCAGCCCGAAGA
  TTCACTATGGTGAAAATCGCCTTCAATACCCCTACCGCCGTGCAAAAGGAGGAGGCGCGG
  CAAGACGTGGAGGCCCTCCTGAGCCGCACGGTCAGAACTCAGATACTGACCGGCAAGGAG
  CTCCGAGTTGCCACCCAGGAAAAAGAGGGCTCCTCTGGGAGATGTATGCTTACTCTCTTA
  GGCCTTTCATTCATCTTGGCAGGACTTATTGTTGGTGGAGCCTGCATTTACAAGTACTTC
  ATGCCCAAGAGCACCATTTACCGTGGAGAGATGTGCTTTTTTGATTCTGAGGATCCTGCA
  AATTCCCTTCGTGGAGGAGAGCCTAACTTCCTGCCTGTGACTGAGGAGGCTGACATTCGT
  GAGGATGACAACATTGCAATCATTGATGTGCCTGTCCCCAGTTTCTCTGATAGTGACCCT
  GCAGCAATTATTCATGACTTTGAAAAGGGAATGACTGCTTACCTGNACTTGTTGCTGGGG
  AACTGCTATCTGATGCCCCTCAATACTTCTATTGTTATGCCTCCAAAAAATCTGGTAGAG
  CTCTTTGGCAACTGGCGAGTGGCAGATATCTGCCTCAACTTATGTGGTTCGAGAAGACCT
  AGTTGCTGTGGAGAAATTCGTGNATGTAGTACCNTGGCATCTTTATTTACCAACTTTGCA
  TAACAGAAAGTCCTTCCGCCTTCGTCGCAGAGACCTCTTGGCTGGNTTCAACAAACGTGC
  CATTGATAAATGCTGGAAGATANACACTTCCCACGAATTTATTGTTGAGACCAGAACTGT
  CAGAGTAGAAGCACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004867 unedited
  GCAACTCTCCAGGTCCAGAAGNAGCACTGGGGCAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTAAAGCATAAGCAATAGAGTAAATGCATGAGTAAATATCTTGAGTATCCCATAAACCT
  TAATGTTAAAGTCATATTGTAAATCTCTGACTTCTTATTACCAAGGACACTCTATCTGTT
  GCCTCTTACTCTTGACAGATCTTGGTCTCAACAATAAATTCGTTGGGGAAGTGTCTAATC
  TTCCAGCATTTATCAATGGCACGTTTGTTGAAACCCAGCAAGAGGTCTCTGCGACGAAGG
  CGGAAGGACTTTCTGTTATTGCAAAGTTGGTAAATAAAGATGCCAAGGTTACTAACATCA
  CGAATTTCCTCCACAGCAACTAGGTCTTCTCGAACCACATAAGTTTGAGGCAGATATCTG
  CCACTCGCCAGTTTGCCAAAGAGCTCTACCAGATTTTTTGGAGGCATAACAATAGAAGTA
  TTGAGGGGCATCAGATAGCAGTTCCCCAGCAACAAGTCCAGGTAAGCAGTCATTCCCTTT
  TCAAAGTCATGAATAATTGCTGCAGGGTCACTATCAGAGAAACTGGGGACAGGCACATCA
  ATGATTGCAATGTTGTCATCCTCACGAATGTCAGCCTNCTCAGTCACAGGCAGGAAAGTA
  GGCTCTCCTCCACGAAGGGAATTTGCANGATCCTCAGATCAAAAAGCACATCTCTCACGG
  TAATGGTGCTCTTGGCATGAAGTACTGTAATGCANGCNNTCACAACATAAGTCTGCCAGA
  AGAATGAAAGGCCTAGAGAGTAGCATCATCTCCCANAGGAGCCTCTTTTNCTGGGTGGGC
  ACTCGACTCCTTGCCGGCANTATCTGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004867
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004867.3, NP_004858.1
• RefSeq Size: 1874 bp
• RefSeq ORF: 792 bp
• Locus ID: 9452
• UniProt ID: O43736
• Cytogenetics: Xq21.1
• Domains: BRICHOS
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a type II membrane protein that belongs to the ITM2 family. Studies in mouse suggest that it may be involved in osteo- and chondrogenic differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (1).