SAE1 (NM_005500) Human Untagged Clone

CAT#: SC116689

SAE1 (untagged)-Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SAE1
• Synonyms: AOS1; HSPC140; SUA1; UBLE1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116689 sequence for NM_005500 edited (data generated by NextGen Sequencing)
  ATGGTGGAGAAGGAGGAGGCTGGCGGCGGCATTAGCGAGGAGGAGGCGGCACAGTATGAC
  CGGCAGATCCGCCTGTGGGGACTGGAGGCCCAGAAACGGCTGCGGGCCTCTCGGGTGCTT
  CTTGTCGGCTTGAAAGGACTTGGGGCTGAAATTGCCAAGAATCTCATCTTGGCAGGAGTG
  AAAGGACTGACCATGCTGGATCACGAACAGGTAACTCCAGAAGATCCCGGAGCTCAGTTC
  TTGATTCGTACTGGGTCTGTTGGCCGAAATAGGGCTGAAGCCTCTTTGGAGCGAGCTCAG
  AATCTCAACCCCATGGTGGATGTGAAGGTGGACACTGAGGATATAGAGAAGAAACCAGAG
  TCATTTTTCACTCAATTCGATGCTGTGTGTCTGACTTGCTGCTCCAGGGATGTCATAGTT
  AAAGTTGACCAGATCTGTCACAAAAATAGCATCAAGTTCTTTACAGGAGATGTTTTTGGC
  TACCATGGATACACATTTGCCAATCTAGGAGAGCATGAGTTTGTAGAGGAGAAAACTAAA
  GTTGCCAAAGTTAGCCAAGGAGTAGAAGATGGGCCCGACACCAAGAGAGCAAAACTTGAT
  TCTTCTGAGACAACGATGGTCAAAAAGAAGGTGGTCTTCTGCCCTGTTAAAGAAGCCCTG
  GAGGTGGACTGGAGCAGTGAGAAAGCAAAGGCTGCTCTGAAGCGCACGACCTCCGACTAC
  TTTCTCCTTCAAGTGCTCTTAAAGTTCCGTACAGATAAAGGAAGAGATCCCAGTTCTGAT
  ACATATGAGGAAGATTCTGAGTTGTTGCTCCAGATACGAAATGATGTGCTTGACTCACTG
  GGTATTAGTCCTGACCTGCTTCCTGAGGACTTTGTCAGGTACTGCTTCTCCGAGATGGCC
  CCAGTGTGTGCGGTGGTTGGAGGGATTTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAG
  CGGGACCCTCCTCACAACAACTTCTTCTTCTTCGATGGCATGAAGGGGAATGGGATTGTG
  GAGTGCCTTGGCCCCAAGTGA
  
  Clone variation with respect to NM_005500.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005500 unedited
  AAATTTGGGATTTTGTAATCCGACTTACTATAGGGNCGGACCGCGCAATTCGGCACGAGG
  CCGGAGCTGAGGCAGGAAGAGCCGGCGCCATGGTGGAGAAGGAGGAGGCTGGCGGCGGCA
  TTAGCGAGGAGGAGGCGGCACAGTATGACCGGCAGATCCGCCTGTGGGGACTGGAGGCCC
  AGAAACGGCTGCGGGCCTCTCGGGTGCTTCTTGTCGGCTTGAAAGGACTTGGGGCTGAAA
  TTGCCAAGAATCTCATCTTGGCAGGAGTGAAAGGACTGACCATGCTGGATCACGAACAGG
  TAACTCCAGAAGATCCCGGAGCTCAGTTCTTGATTCGTACTGGGTCTGTTGGCCGAAATA
  GGGCTGAAGCCTCTTTGGAGCGAGCTCAGAATCTCAACCCCATGGTGGATGTGAAGGTGG
  ACACTGAGGATATAGAGAAGAAACCAGAGTCATTTTTCACTCAATTCGATGCTGTGTGTC
  TGACTTGCTGCTCCAGGGATGTCATAGTTAAAGTTGACCAGATCTGTCACAAAAATAGCA
  TCAAGTTCTTTACAGGAGATGTTTTTGGCTACCATGGATACACATTTGCCAATCTAGGAG
  AGCATGAGTTTGTAGAGGAGAAAACTAAAGTTGCCAAAGTTAGCCAAGGAGTAGAAGATG
  GGCCCGACACCAAGAGAGCAAAACTTGATTCTTCTGAGACAACGATGGTCAAAAAGAAGG
  TGGTCTTCTGCCCTGTTAAAGAAGCCCTGNGAGTGGACTGGAGCAGTGAGAAAGCAAGGC
  TGCTCTGAAGCGCACGACCTNCGACTACTTTCTCCTTNCAGTGCTCTTAAAGTTCCGTAC
  AGATNAAGGAAGAGATCCCAGTTCTGATACATATGAAGAAAGATTCTGAGTGNTGCTNCA
  GATACGAATGATGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005500 unedited
  AGAGTCGAGTTTTTTTTTTTTTTTTTTCCTAAAGCAAGTAACTTTATTATCATTCCTTTA
  AAAAGAACCAAGGAAAATTCACAACATATGTGAAACACAAACAGCTGTGGTTTAGGAGGT
  AAACAAAGGACCAACATAGCCCTGAAATGCAACAGCCTCTGAGTGACTTGAGCCGCATGT
  GACTGGGGTTCTGTTAAAAGGGCAGGCTCCTCCCTCCTAGCCCTGAAGCCCCAGGAACCT
  GCCTTGAAAGACAAGCTCTCTAATACTCAACTTGCAGGGTCTCGCCCTAACATCCAAGAC
  TTGGTAGCATCTCCTTTCTCCCAAAACCCAGCTGGAACTCAACTAATCCTAAACGAAAAC
  TCAAGAACAGCACACCAGATGCCACCTGTTGTTTGTCAGGGTCTCAAACTCCAGAGGAAA
  TGCATTTGCCTGTCATGGTTTTCCTCTCTAAGGGCACCTGTCTGAACTTGGAGCTGTGCT
  TCCACTGGTGCCTCATGGNGCCTGGGCCTACTCTCCACCTGAATTCACACTTTTCTCTAT
  AGGTATCAGGTGGGGAATAAGTCCAAGAAGGTGACAAGAGGCTCCCGAGCACAGAGCTTG
  CTCATTCCTGNCANGCATCTCCCACAATGCCCCANNAGGGCTGCGAGGCCCGGNACAGCA
  TANGGACAGAGAAAGGGCANAGGGGTGCTTGGAAGTGCTCATCACTCANGGGAGCTGCCA
  GGCCCCGGATGACANGCANCCTGAACAGTGCTGGAAACAAGACAGNCACCCTGCGCCCCT
  TGTCNAGCANCTGCTTNTGATGGGAAANCACACGTCGNCACCACCTTCTTCGTGCANGAA
  ATGNTNTGTATCGGGCCCATGACTCAGTTTNCCTTGCTGGAGATGCCTTCATGAAGAAGG
  NGACAGGNATACAGNTGGCATGCTGCANTGGCATCTCTGGGCTGCAAAACTTGGNTACTT
  GGGGCANGACTNACAATCCATTCCTTATGCTCANAAAAAAAATGTGNAGAGAGGTCGCTA
  AAAGGCTNCAATNCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005500
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005500.1, NP_005491.1
• RefSeq Size: 2119 bp
• RefSeq ORF: 1041 bp
• Locus ID: 10055
• UniProt ID: Q9UBE0
• Cytogenetics: 19q13.32
• Domains: ThiF
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (a).