S100A12 (NM_005621) Human Untagged Clone

CAT#: SC116641

S100A12 (untagged)-Human S100 calcium binding protein A12 (S100A12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S100A12
• Synonyms: CAAF1; CAGC; CGRP; ENRAGE; MRP-6; MRP6; p6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116641 sequence for NM_005621 edited (data generated by NextGen Sequencing)
  ATGACAAAACTTGAAGAGCATCTGGAGGGAATTGTCAATATCTTCCACCAATACTCAGTT
  CGGAAGGGGCATTTTGACACCCTCTCTAAGGGTGAGCTGAAGCAGCTGCTTACAAAGGAG
  CTTGCAAACACCATCAAGAATATCAAAGATAAAGCTGTCATTGATGAAATATTCCAAGGC
  CTGGATGCTAATCAAGATGAACAGGTCGACTTTCAAGAATTCATATCCCTGGTAGCCATT
  GCGCTGAAGGCTGCCCATTACCACACCCACAAAGAGTAG
  
  Clone variation with respect to NM_005621.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005621 unedited
  TTCGGCTTTTGTAACACGACTCACTAAGGGCGGCACGCGATTCGGCACGAGGTAGCTCCA
  CATTCCTGTGCATTGAGGGGTTAACATTAGGCTGGGAAGATGACAAAACTTGAAGAGCAT
  CTGGAGGGAATTGTCAATATCTTCCACCAATACTCAGTTCGGAAGGGGCATTTTGACACC
  CTCTCTAAGGGTGAGCTGAAGCAGCTGCTTACAAAGGAGCTTGCAAACACCATCAAGAAT
  ATCAAAGATAAAGCTGTCATTGATGAAATATTCCAAGGCCTGGATGCTAATCAAGATGAA
  CAGGTCGACTTTCAAGAATTCATATCCCTGGTAGCCATTGCGCTGAAGGCTGCCCATTAC
  CACACCCACAAAGAGTAGGTAGCTCTCTGAAGGCTTTTTACCCAGCAATGTCCTCAATGA
  GGGTCTTTTCTTTCCCTCACCAAAACCCAGCCTTGCCCGTGGGGAGTAAGAGTTAATAAA
  CACACTCACGAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGC
  TGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGC
  CNTAGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCA
  TTTTGTCTGACTAGGTGTCCTTCTATAATATATGGGGTGGGGGGGGGGNNNNNNNNNNNN
  NNGGNNGGGGGNGGGGNGGGGGGNGNNNNNNNGNNGGGGNNGGGGGNGNNNNNNGNNGGN
  NNNGGNGNNGNGGNNNGGGNGGGGGGNGGGGNGGGGNNNGGGGGGGGGNNNGNNNNGGGN
  NNGGGGNNNNNNNNNNNNGNNNNGGNNNGGGGGGNNGGNNNGGGNNGNNNNNNNNGNNNN
  NNNNGGGGNNGGNGGNNGNGGGNGNGGGGGNNNGGGGGGGGGGGGGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005621 unedited
  CGGGGCCCCNNNNNNNNNNNNNNCNNNNNNNNNTTTATTTTAGGACCCCGGNCCGCATAC
  TANGATCGAGTTTTTTTTTTTTTTTTTTTCGTGAATGTGTTTATTAACTCTTACTCCCCC
  GGGCAAGGCTGGGTTTTGGTGAGGGAAAGAAAAGACCCTCATTGAGGACATTGCTGGGTA
  AAAAGCCTTCAGAGAGCTACCTACTCTTTGTGGGTGTGGTAATGGGCAGCCTTCAGCGCA
  ATGGCTACCAGGGATATGAATTCTTGAAAGTCGACCTGTTCATCTTGATTAGCATCCAGG
  CCTTGGAATATTTCATCAATGACAGCTTTATCTTTGATATTCTTGATGGTGTTTGCAAGC
  TCCTTTGTAAGCAGCTGCTTCAGCTCACCCTTAGAGAGGGTGTCAAAATGCCCCTTCCGA
  ACTGAGTATTGGTGGAAGATATTGACAATTCCCTCCAGATGCTCTTCAAGTTTTGTCATC
  TTCCCAGCCTAATGTTAACCCCTCAATGCACAGGAATGTGGAGCTACCTCGTGCCGAATT
  CGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTG
  CTTATATAGACCTCCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGCCGGGNTAT
  TACGACATTTTGGAAAGTCCCNGTGATTTTGGTGCCAAAACNAACTCCNATTGACGTCAA
  TGGGTTGGAGACTGGNAAATCCCGTGAGTCAAACCGCTATCCACGCCCATTTGTGTACTG
  CCAAACCCGATTACTTGGGAATAGCGATGACTAAACGTANATGACCTGCCAAGTAGAAAG
  TCCGTTAGTCATGTACTGGGCATATGCCAGGCGGCCATTTACCGCATGACGTAATAAGGG
  GCGGACTTGCAATGAACCACTTGAGCCTGCCAGTGGGCAGTTTACCGTAAAACTCACCCT
  TGACGTCAGGGAAGCCCTTTGCGGTCCTTTGGAACATCCTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005621
• Insert Size: 480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005621.1, NP_005612.1
• RefSeq Size: 466 bp
• RefSeq ORF: 279 bp
• Locus ID: 6283
• UniProt ID: P80511
• Cytogenetics: 1q21.3
• Domains: S_100, EFh
• Gene Summary: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]