NELL2 (NM_006159) Human Untagged Clone

CAT#: SC116281

NELL2 (untagged)-Human NEL-like 2 (chicken) (NELL2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NELL2
• Synonyms: NRP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116281 sequence for NM_006159 edited (data generated by NextGen Sequencing)
  ATGGAGTCTCGGGTCTTACTGAGAACATTCTGTTTGATCTTCGGTCTCGGAGCAGTTTGG
  GGGCTTGGTGTGGACCCTTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAG
  TCCACGACCGGAGTGCGTCAGGTCCCGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTT
  CAAGATACTCCCAGAAGCATAAAAGCATCCACTGCTACAGCTGAACAGTTTTTTCAGAAG
  CTGAGAAATAAACATGAATTTACTATTTTGGTGACCCTAAAACAGACCCACTTAAATTCA
  GGAGTTATTCTCTCAATTCACCACTTGGATCACAGGTACCTGGAACTGGAAAGTAGTGGC
  CATCGGAATGAAGTCAGACTGCATTACCGCTCAGGCAGTCACCGCCCTCACACAGAAGTG
  TTTCCTTACATTTTGGCTGATGACAAGTGGCACAAGCTCTCCTTAGCCATCAGTGCTTCC
  CATTTGATTTTACACATTGACTGCAATAAAATTTATGAAAGGGTAGTAGAAAAGCCCTCC
  ACAGACTTGCCTCTAGGCACAACATTTTGGCTAGGACAGAGAAATAATGCGCATGGATAT
  TTTAAGGGTATAATGCAAGATGTCCAATTACTTGTCATGCCCCAGGGATTTATTGCTCAG
  TGCCCAGATCTTAATCGCACCTGTCCAACTTGCAATGACTTCCATGGACTTGTGCAGAAA
  ATCATGGAGCTACAGGATATTTTAGCCAAAACATCAGCCAAGCTGTCTCGAGCTGAACAG
  CGAATGAATAGATTGGATCAGTGCTATTGTGAAAGGACTTGCACCATGAAGGGAACCACC
  TACCGAGAATTTGAGTCCTGGATAGACGGCTGTAAGAACTGCACATGCCTGAATGGAACC
  ATCCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGGCTCTTGCG
  TATGTGGATGGCAAATGCTGTAAGGAATGCAAATCGATATGCCAATTTCAAGGACGAACC
  TACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTCTGGAGTATGTGTTCTCTATGAG
  TGCAAGGACCAGACCATGAAACTTGTTGAGAGTTCAGGCTGTCCAGCTTTGGATTGTCCA
  GAGTCTCATCAGATAACCTTGTCTCACAGCTGTTGCAAAGTTTGTAAAGGTTATGACTTT
  TGTTCTGAAAGGCATAACTGCATGGAGAATTCCATCTGCAGAAATCTGAATGACAGGGCT
  GTTTGTAGCTGTCGAGATGGTTTTAGGGCTCTTCGAGAGGATAATGCCTACTGTGAAGAC
  ATCGATGAGTGTGCTGAAGGGCGCCATTACTGTCGTGAAAATACAATGTGTGTCAACACC
  CCGGGTTCTTTTATGTGCATCTGCAAAACTGGATACATCAGAATTGATGATTATTCATGT
  ACAGAACATGATGAGTGTATCACAAATCAGCACAACTGTGATGAAAATGCTTTATGCTTC
  AACACTGTTGGAGGACACAACTGTGTTTGCAAGCCGGGCTATACAGGGAATGGAACGACA
  TGCAAAGCATTTTGCAAAGATGGCTGTAGGAATGGAGGAGCCTGTATTGCCGCTAATGTG
  TGTGCCTGCCCACAAGGCTTCACTGGACCCAGCTGTGAAACGGACATTGATGAATGCTCT
  GATGGTTTTGTTCAATGTGACAGTCGTGCTAATTGCATTAACCTGCCTGGATGGTACCAC
  TGTGAGTGCAGAGATGGCTACCATGACAATGGGATGTTTTCACCAAGTGGAGAATCGTGT
  GAAGATATTGATGAGTGTGGGACCGGGAGGCACAGCTGTGCCAATGATACCATTTGCTTC
  AATTTGGATGGCGGATATGATTGTCGATGTCCTCATGGAAAGAATTGCACAGGGGACTGC
  ATCCATGATGGAAAAGTTAAGCACAATGGTCAGATTTGGGTGTTGGAAAATGACAGGTGC
  TCTGTGTGCTCATGTCAGAATGGATTCGTTATGTGTCGACGGATGGTCTGTGACTGTGAG
  AATCCCACAGTTGATCTTTTTTGCTGCCCTGAATGTGACCCAAGGCTTAGTAGTCAGTGC
  CTCCATCAAAATGGGGAAACTTTGTATAACAGTGGTGACACCTGGGTCCAGAATTGTCAA
  CAGTGCCGCTGCTTGCAAGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAG
  TGTGAATTCAGCATTCTCCCAGAGAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGC
  CAGGCTGACACCATCCGCAATGACATCACCAAGACTTGCCTGGACGAAATGAATGTGGTT
  CGCTTCACCGGGTCCTCTTGGATCAAACATGGCACTGAGTGTACTCTCTGCCAGTGCAAG
  AATGGCCACATCTGTTGCTCAGTGGATCCACAGTGCCTTCAGGAACTGTGA
  
  Clone variation with respect to NM_006159.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006159 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGGAAAACGGGGGGGTG
  CGGGGCCGCCCCAGCCCGGGCTTTCTTGGGGCCGCCCCCCTTCTACCGGGTGTGCGAGTC
  TTTGGCTGCTTTTATTCGGCTCGGGAGCTAATTCCCCGACGGAGCCGCGCCGGGGCGAGT
  CCGACCCCTCCCTCCGGGCCCCCTCCGGGCCGCGCTGCCGCCTCGGCCCTGCGTGTGGGA
  ATGATGTGCGCATTGGAGGGTCTAAGTTCTTCACGCGCCTGGGGAGGCCTCCCTTTTCTT
  TCTTAGGCAACCAAAGCGTATTAATCCTACTGATCAGTAAATCCGAGGCAGCAGCAGGAG
  AGACAAACGTTATTTTCCCGCTTGATTCCAAGAACCTCTTCGATTTTTATTTTTATTTTT
  AAAGAGGGAGACGATGGACTGAGCTGATCCGCACCATGGAGTCTCGGGTCTTACTGAGAA
  CATTCTGTTTGATCTTCGGTCTCGGAGCAGTTTGGGGGCTTGGTGTGGACCCTTCCCTAC
  AGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCCACGACCGGAGTGCGTCAGGTCC
  CGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTTCAAGATACTCCCAGAAGCATAAAAG
  CATCCACTGCTACAGCTGAACAGTTNTTTCAGAAGCTGAGAAATAAACATGAATTTACTA
  TTTTTGGTGACCCCTAAACAGACCCACTTAAATTCAGGGAGTATTCTCTCAATCACCACT
  TGGATCACAGGTACCTGGGACTGGNAAAGTAGTGGCCATCGGAATGAAGTCAGACTGCAT
  TACCGCTCAGGCAGTCACCGCCCTCACACAAAANGTTTTCCTACATTTTGGCTGAGGACA
  GATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006159 unedited
  GCGGCCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGGTAAGAGAATATTTATT
  TAATTTTGCACAAGAACGAGCTTATACTGAACAAATTCAACCAAATAATATTAAGTGCAG
  TAAAACAAGGAAATAGGGATGACTTTTCCTGTTAGAAAATATTAAGTAACCATGCCTGTG
  CATTATTTTTATCATTACAATAACTTGTTTCAGATTTCTTTTCAGAGTGTACTGTACATA
  ACATGTTATAGCTTCTTTGCTTTACTTCTGAAAATATAATTCATTCACTGCCTGACATTT
  TATTTCTTGCAGTGAGGAACCTAACTGATTCTTGAAGATTCTGATCTAGAGGAAAATTTT
  CTGCACCAGTGAAGCTAGAGGCTTTCACAGATCCAATGGGCTCAGGCTTTCTATCCAGGG
  TTCAGGATGTCACGGTATATACTGTACGCCCATTCTTCTACATGGTGATGTGAGACACAG
  TAGAGGCAGACTTGAGGTCTAATTTTGCCCCAGTAATTTTCCTTTTGTGATTTTGTCAAG
  CTCCACAAATTTCATAATTGAAAGTTCACTCAGCTATTAACAAAGCTGCATTTAGCTGCC
  CACAAATCACCCAATTTAAGTTTTAACTTCTTTTTGGTCTTTTAATGAAAGAACATTCTT
  TTAACAGAAATCTCCCATGAGACAGTTAACTTCACAGTTCCCTGAAGCACTGTGGATCCA
  CTGAGCAACAGATGTGGCCATTCTTGCACTGGCAGAGAGTACACTCAGTGCCATGTTTTG
  ATTCAAGATGACCCCGGTGAAGCGAACACATTTATTTCCTCAAGCCAGTCTTGGTGATGT
  TATTGCGGATGGTGTCACCCTGCGAAGGGTCTGTGACCCACGGCGGCAACACTCATCTTT
  GGAAGATGCTGAATCACCCTCCCNTTTGGGCAAGGCAGGGCCACAATAACTTTCCTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006159
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006159.1, NP_006150.1
• RefSeq Size: 3198 bp
• RefSeq ORF: 2451 bp
• Locus ID: 4753
• UniProt ID: Q99435
• Cytogenetics: 12q12
• Domains: VWC, LamG, EGF_CA, TSPN, EGF, EGF, VWC_out
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode isoform b.