PPP2R5A (NM_006243) Human Untagged Clone

CAT#: SC116240

PPP2R5A (untagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5A
• Synonyms: B56A; B56alpha; PR61A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116240 sequence for NM_006243 edited (data generated by NextGen Sequencing)
  ATGTCGTCGTCGTCGCCGCCGGCGGGGGCTGCCAGCGCCGCCATCTCGGCCTCGGAGAAA
  GTGGACGGCTTCACCCGGAAATCGGTCCGCAAGGCGCAGAGGCAGAAGCGCTCCCAGGGC
  TCGTCGCAGTTTCGCAGCCAGGGCAGCCAGGCAGAGCTGCACCCGCTGCCCCAGCTCAAA
  GATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATA
  CTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACA
  CTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTAT
  TCTGATATAGTAAAAATGATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAAT
  CCAGATTTTGATCCAGAAGAGGATGAACCCACGCTTGAGGCCTCTTGGCCTCACATACAG
  TTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGCCCTGATTTCCAGCCTAGCATTGCA
  AAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGATAGTGAAGAT
  CCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGA
  TTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACA
  GAACATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTT
  GCATTGCCACTGAAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCAT
  ACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTG
  GAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACTGCTGAAATTTTGGCCAAAA
  ACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTAGATGTCATT
  GAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTA
  TCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATT
  CTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTAC
  AAAATTTCCAAAGAACACTGGAATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAA
  ACCCTAATGGAAATGAATGGCAAGCTTTTCGATGACCTTACTAGCTCATACAAAGCTGAA
  AGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGAAGAATTATGGAAAAAATTAGAGGAG
  CTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAGTGCTTACAACATGCACAGTATTCTC
  AGCAATACAAGTGCCGAATAA
  
  Clone variation with respect to NM_006243.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006243 unedited
  NTGTTCAAATATTTGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGTCTC
  AAGTTGTAGCGGTCGCTCGCCTGGGGTTCTCCGTGGGCGGCCGACGGGCGCGTGGGGGAG
  GGGGTTCGGGCCGCCCAGCGCGGCAAGGAGCGAGTGTGTGCACGCAGAGGGCCGGGGCTA
  CGGGGCAGCGCCCCGGGCGATGAGGGGCCGGCGTTGACCGGGAAGAGCGGGCACCGCGGC
  AGTGGCTCCGAGGGGACCCGCGATGGCAGCGCCCTGAGAGGAGGCTCCAGGCAGGGCGGG
  CTGCGCTGGCAGCGGCCGCTGAGGTGCTGGCCGGCCGGCTGGCTGGCGACGGGGGCAGAA
  GCGACGAGAGGCGCGCTCGGCACCCGCACCCCCGTGCCCCCGCCTCAGTTGTCTAAACTT
  CGGGCTCTCTTCCACCCGCTCTGCGCGCCCAGAGTCAACAACTTCTTCACCCCCCTCCGC
  CCCCGCCCTTCCCTCCGTCAGCCCCGGGAGCTCGCCGCGCGCCGGGGACCAGGAACCTCC
  AGCGCTGAGATGTGGCCGTGAGGCGTTGGCGGGCGGCGAGGAGAAGCTCGGCGGCGTCCC
  GGGGCCGGAGGGCCGTGGGGCCGGGGCGCAGGGGCGCGAGCACCCCGCGCCTCTCCCCCG
  CCTCCTCCTGCCGTCTCCGCCGCTGCCCGTGCCTTGCAAGCAGCAGCCGGAGCTGCCAAG
  CGTCAGGGCCGCGGGAGAATGTCGTCGTCGTCGCCGCCGGCGGGNGGCTGCCAGCGCCGC
  CATCTCGGCCTCGGAGAAAGTGGACGGCTTCACCCGGAAAATCGTCCGCAAGGCGCANAG
  GCAGAAGCGCTCCCAGGCTCGGTCGCAGTTTCGCACCAGGGCAGCCAGGCAGAGCTGCAC
  CGCTGCCCAGCTCNAAGAGCCACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006243 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTCAAATACAAACATAGACTTTA
  TTAAATGCTGCTCAATCCCCCAATGAAGATCTTTCATTACAAAACAGTTTTCCACACAAC
  TGCAAGTTAAAAGATTGGAATGGTACTCTGATAACAAAAGATGTGAGAAATACTTGACCA
  AGTAAAAACATACAAGACCTCTATATCGTCAACCTTTTCAAAGCTCTACAGAGGAAGCCT
  ACTGCAGTTCCTTTAAATAAAGCCAGGCAGTGACAACTAGTGCTCTGTATCACCACACTG
  GGGGACTCAAAGCCTCCATCCCTGGGTCAGGAGGGACTAGGTGAAGGACAGGTTGTGAGG
  ACTGGAAAGGCCCTGCCTTCAGCCTTTGAGAGGAAGAGGAGGAATGAATTGAGAGGCAAG
  CTGGGTAATTCACCTGAGCTGCCTCTACCCATCTGTTACCACTCTGGAGAATTGCAGAGG
  AAGGGGGATATTCATGAGGAACAATCTGAAGAATTTTGGTTTACTTTCCCAGCTTTTATT
  TATATTTTGTGCTTTAAAAAGCCTGGAGATTTAAGGTTTTGCCTAAATTCCAGAGTACAG
  TAATGTATTTATTGAGGGAGGGGTTAGGGCAGGTAATTCCCCCCATCATATTTCTGGGTG
  AGACAAAGAGAAGGGTAAGGATGATACCTTAGTTTTATTTTAGGAAGTATTTTTTCCTAG
  GATGTAACTAAGGCTCGCCGCAGTGTGAAAACCCATTATAAAGGGGAAATTCTCAGAGCC
  GACTACCGTATCTTTGTCCCACACCATCAAGGAAAACTTAAGCCATAATTCTTACCCCTC
  CATAATATCCTCCTCATTTCATAATGAAAAACCCTTCATTTCTGTGAAATTTCGAAGGAC
  ACCTAAACTTACGCATAAACAGGGTACATCTCTATTGCCTGAACAGCCACGGCCCGCTTA
  ACCTATATCCGCCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006243
• Insert Size: 3190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006243.2, NP_006234.1
• RefSeq Size: 3145 bp
• RefSeq ORF: 1461 bp
• Locus ID: 5525
• UniProt ID: Q15172
• Cytogenetics: 1q32.3
• Domains: B56
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.