TAF10 (NM_006284) Human Untagged Clone

CAT#: SC116191

TAF10 (untagged)-Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF10
• Synonyms: TAF2A; TAF2H; TAFII30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006284, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCTGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGCCGGCCTCCGCCGCCTCGGCCCCGGGCCCCG
  CGCCCCCGGTCTCGGCTCCCGCCGCGCTGCCCTCCAGCACCGCCGCGGAGAACAAGGCCAGCCCCGCGGG
  GACAGCGGGGGGACCTGGGGCTGGAGCAGCTGCTGGGGGCACGGGACCCTTGGCGGCGCGGGCCGGGGAG
  CCAGCTGAGCGGCGTGGGGCGGCTCCGGTGTCGGCGGGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTA
  ACGGGGTTTACGTACTGCCGAGCGCGGCCAACGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGT
  GGACTTCTTGATGCAGCTGGAAGATTACACGCCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAAC
  CGTGCTGGCTTTGAGGCCTCAGACCCACGCATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTCATCT
  CAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACGGCCTCCGGCAGCTCCCGGAGCAA
  GAGCAAGGACCGCAAGTACACTCTAACCATGGAGGACTTGACCCCTGCCCTCAGCGAGTATGGCATCAAT
  GTGAAGAAGCCGCACTACTTCACCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006284 unedited
  TTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCCGGCCCGATGAGC
  TGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGCCGGCCTCCGCCGCCTCGGCCCCGGGC
  CCCGCGCCCCCGGTCTCGGCTCCCGCCGCGCTGCCCTCCAGCACCGCCGCGGAGAACAAG
  GCCAGCCCCGCGGGGACAGCGGGGGGACCTGGGGCTGGAGCAGCTGCTGGGGGCACGGGA
  CCCTTGGCGGCGCGGGCCGGGGAGCCAGCTGAGCGGCGTGGGGCGGCTCCGGTGTCGGCG
  GGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTAACGGGGTTTACGTACTGCCGAGCGCG
  GCCAACGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGTGGACTTCTTGATGCAG
  CTGGAAGATTACACGCCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAACCGTGCT
  GGCTTTGAGGCCTCAGACCCACGCATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTC
  ATCTCAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACAGCCTCCGGC
  AGCTCCCGGAGCAAAGAGCAGGACCGCAAGTACACTCTAACCATGGAGGACTTGACCCCT
  GCCCTCAGCGAGTATGGCATCAATGTGAAGAAGCCGCACTACTTCACCTGAGCCACCCAA
  CCTAAATGTACTTATCTGTCCCCATGTCCCCACACCAGCCTGTTTTCATAAATAAACGTT
  ATGTGACAGGCCNNNNNNNNNNNNNTNNNNNTNNNNNNGNCCCNNNACTCCAACCCCTAT
  TNCCCTAAACAAAATATTTCTCTTGCCACTTTAAAAATCAAAAAACCCCCCCCTCTCTAA
  TTGCGGCCNGGGATAAGTGTTTCTGAAAAAACCGGGGGCACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006284 unedited
  GGGGGGNNNNGGGGGGNNNNNNNNNGTNNNANNTTNTNNNNNNNNGGGTTTTTCTGTGCC
  GATAGAAGATTTCTTNATGTTAAAACAGNNCTGGTGTGGGGNACATGGGGNACAGCAAAG
  TACATTTAGGTTGGGTGGCTCAGGTGAAGTAGTGCGGCTTCTTCACATTGATGCCATACT
  CGCTGAGGGCAGGGGTCAAGTCCTCCATGGTTAGAGTGTACTTGCGGTCCTTGCTCTTGC
  TCCGGGAGCTGCCGGAGGCTGTGCCCTTCATTTTGCAGTGCTGTAGGGCATCATTGGCAA
  TATCTGAGATGAATTTCTGGGCAGCTAAGGAGATGAGCCGAATTATGCGTGGGTCTGAGG
  CCTCAAAGCCAGCACGGTTCAGGTAGTAACCAGTCACTGCATCTGGGATCGTAGGCGTGT
  AATCTTCCAGCTGCATCAAGAAGTCCACCAAAGGCGTGCTGGACACCACGGGCTTCACGT
  CTCCGTTGGCCGCGCTCGGCAGTACGTAAACCCCGTTAGATATGGCCCCCTCCGGGGGCG
  CCGCGCCACCCGCCGACACCGGAGCCGTCCCACGCCGCTCAGCTGGCTCCCCGGCCCGCG
  CCGCCAAGGGTCCCGTGCCCCCAGCAGCTGCTCCAGCCCCAGGTCCCCCCGCTGTCCCCG
  CGGGGCTGGCCTTGTTCTCCGCGGCGGTGCTGGAGGGCACCGCGGCGGCAGCCGATACCG
  GGGGCGCGGGGCCCCGGGGCCGAGGCGGCGGATGCCGTCGCCGCCTTGGGGTCCCGCGCC
  GTACCGTTGTAGCTTATCGGGCCCGGCCTTGTGTCCGAATTCCTGGCCGCCCTATAGTGA
  GTCGTATTACAAAATTCTGCACGGTTCACTAAACGAACCTTTGCTTATATAACACCTCCA
  CCCGTTCACGCCTTACCCCCCTTTTGGCGTAAACGGGGCGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006284
• Insert Size: 3760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006284.2, NP_006275.1
• RefSeq Size: 762 bp
• RefSeq ORF: 657 bp
• Locus ID: 6881
• UniProt ID: Q12962
• Cytogenetics: 11p15.4
• Domains: TFIID_30kD
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008]