TXNRD2 (NM_006440) Human Untagged Clone

CAT#: SC116122

TXNRD2 (untagged)-Human thioredoxin reductase 2 (TXNRD2), nuclear gene encoding mitochondrial protein (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: TXNRD2
• Synonyms: GCCD5; SELZ; TR; TR-BETA; TR3; TRXR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116122 sequence for NM_006440 edited (data generated by NextGen Sequencing)
  ATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGGACGCAG
  GCCGTGGCGGGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGGGACTAT
  GATCTCCTGGTGGTCGGCGGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCCGCTCAG
  CTGGGAAGGAAGGTGTCCGTGGTGGACTACGTGGAACCTTCTCCCCAAGGCACCCGGTGG
  GGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCAAGAAGCTGATGCACCAGGCG
  GCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGTGGCCCAGCCC
  GTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCCTTGAAC
  TGGGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAAGCCAGC
  TTTGTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTGCTGTCA
  GCCGATCACATCATCATTGCTACTGGAGGGCGGCCGAGATACCCCACGCACATCGAAGGT
  GCCTTGGAATATGGAATCACAAGTGATGACATCTTCTGGCTGAAGGAATCCCCTGGAAAA
  ACGTTGGTGGTCGGGGCCAGCTATGTGGCCCTGGAGTGTGCTGGCTTCCTCACCGGGATT
  GGGCTGGACACCACCATCATGATGCGCAGCATCCCCCTCCGCGGCTTCGACCAGCAAATG
  TCCTCCATGGTCATAGAGCACATGGCATCTCATGGCACCCGGTTCCTGAGGGGCTGTGCC
  CCCTCGCGGGTCAGGAGGCTCCCTGATGGCCAGCTGCAGGTCACCTGGGAGGACAGCACC
  ACCGGCAAGGAGGACACGGGCACCTTTGACACCGTCCTGTGGGCCATAGGTCGAGTCCCA
  GACACCAGAAGTCTGAATTTGGAGAAGGCTGGGGTAGATACTAGCCCCGACACTCAGAAG
  ATCCTGGTGGACTCCCGGGAAGCCACCTCTGTGCCCCACATCTACGCCATTGGTGACGTG
  GTGGAGGGGCGGCCTGAGCTGACACCCACAGCGATCATGGCCGGGAGGCTCCTGGTGCAG
  CGGCTCTTCGGCGGGTCCTCAGATCTGATGGACTACGACAATGTTCCCACGACCGTCTTC
  ACCCCGCTGGAGTATGGCTGTGTGGGGCTGTCCGAGGAGGAGGCAGTGGCTCGCCACGGG
  CAGGAGCATGTTGAGGTCTATCACGCCCATTATAAACCACTGGAGTTCACGGTGGCTGGA
  CGAGATGCATCCCAGTGTTATGTAAAGATGGTGTGCCTGAGGGAGCCCCCACAGCTGGTG
  CTGGGCCTGCATTTCCTTGGCCCCAACGCAGGCGAAGTTACTCAAGGATTTGCTCTGGGG
  ATCAAGTGTGGGGCTTCCTATGCGCAGGTGATGCGGACCGTGGGTATCCATCCCACATGC
  TCTGAGGAGGTAGTCAAGCTGCGCATCTCCAAGCGCTCAGGCCTGGACCCCACGGTGACA
  GGCTGCTGAGGGTAA
  
  Clone variation with respect to NM_006440.3
  177 c=>t;196 g=>t;1109 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006440 unedited
  CCGGGCCCCCCCNNCCCNCCCCCCCCTCCCCCCCCCCCNCCCCCGTTCAAATTTGTNATA
  CGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCAGCCCTAGCTGCCCCAGAAGCCCC
  ACGACGATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGG
  ACGCAGGCCGTGGCGGGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGG
  GACTATGATCTCCTGGTGGTCGGCGGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCC
  GCTCAGCTGGGAAGGAAGGTGTCCGTGGTGGACTACGTGGAACCTTCTCCCCAAGGCACC
  CGGTGGGGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCAAGAAGCTGATGCAC
  CAGGCGGCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGTGGCC
  CAGCCCGTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCC
  TTGAACTGNGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAA
  GCCAGCTTTGTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTG
  CTGTCAGCCGATCACATCATCATTGCTACTGGNAGGCGGCCGAGATACCCCACGCACATC
  GAAGGTGCCTTGGAATATGGAAATCACAGTGATGACATCTTCTGGCTGAAGGAAATCCCT
  GGNAANACGTNGNTGGGTCGGGCCAGCTATGTGGNCCTGGAGTGTGCTGGCTTTCTCACC
  GGGATTGGGGCTGGACACACATCATGATGCGCAGCATCCCCCTTCGCGGNTTTCGACAGC
  AAAGGTNCTCCATGGTCATAGAACACATGGCATCTCATGGCACCCGGNTCCTGAGGGGNC
  TGGGGCCCCCTCCCGGGTCAGGAAGCCTTCCTGATGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006440 unedited
  TTTAAAACGGTCCTACTATGNACCGCGCCGCAATCTAAATCGAGTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTCACACTTACAAAAAAAGTACCCTCTTTATTTGCATTGCAAAAATGC
  CAGGGGCGCTCGGACATGGGTGCCCACAGGGTAGGTTTCCACCCAGGTCGTCATCATCTG
  GCACCAGGAGCCTGGGGGGGGGTGCCCGGCGGCAAAGGCTGAAAGGTGCAGGGGTGAGGT
  GGACAGTCCCCTGAGCCATCCCTGTGGGGCACACGGGCCACCTGTCCAGCACTTGCGCTC
  CACGCTGACCATCTCACAGGCGCTCCAGGAGAGGGTTGAGGCCCCCCAAACCTGGCCTGC
  AGCCATCCTGGGTCTGGCCTCCGAGGAGCTGGCGGCGGGCGCACCGTGTGCCCTGGCCTG
  CAGGGATGGCGCTTACCCTCAGCAGCCTGTCACCGTGGGGTCCAGGCCTGAGCGCTTGGA
  GATGCGCAGCTTGACTACCTCCTCAGAGCATGTGGGATGGATACCCACGGTCCGCATCAC
  CTGCGCATAGGAAGCCCCACACTTGATCCCCAGAGCAAATCCTTGAGTAACTTCGCCTGC
  GTTGGGGCCAAGGAAATGCAGGCCCAGCACCAGCTGTGGGGGCTCCCTCAGGCACACCAT
  CTTTACATAACACTGGGATGCATCTCGTCCAGCCACCGTGAACTCCAGTGGTTTATAATG
  GGCGTGATAGACCTCAACATGCTCCTGCCCGTGGCGAGCCACTGCCCTCTTCTTCGACAG
  CCCCACACAGCCTACTCCACGGGGTAGAAAACGGTCGGGGGAACATTGTCGTAGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006440
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006440.2, NP_006431.2
• RefSeq Size: 2180 bp
• Locus ID: 10587
• UniProt ID: Q9NNW7
• Cytogenetics: 22q11.21
• Domains: pyr_redox, pyr_redox_dim
• Protein Families: Druggable Genome
• Protein Pathways: Pyrimidine metabolism
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. [provided by RefSeq, Jun 2017]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1, also known as hTR3a). This isoform is localized to the mitochondria (PMID:16774913). CCDS Note: This CCDS ID represents the protein described in PMIDs: 9923614 and 10215850. This transcript is supported by AF106697.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 9923614. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein.