PHAPI2 / APRIL (ANP32B) (NM_006401) Human Untagged Clone

CAT#: SC116090

ANP32B (untagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHAPI2 / APRIL
• Synonyms: APRIL; PHAPI2; SSP29
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116090 sequence for NM_006401 edited (data generated by NextGen Sequencing)
  ATGGACATGAAGAGGAGGATCCACCTGGAGCTGAGGAACCGGACCCCGGCAGCTGTTCGA
  GAACTTGTCTTGGACAATTGCAAATCAAATGATGGAAAAATTGAGGGCTTAACAGCTGAA
  TTTGTGAACTTAGAGTTCCTCAGTTTAATAAATGTAGGCTTGATCTCAGTTTCAAATCTC
  CCCAAGCTGCCTAAATTGAAAAAGCTTGAACTCAGTGAAAATAGAATCTTTGGAGGTCTG
  GACATGTTAGCTGAAAAACTTCCAAATCTCACACATCTAAACTTAAGTGGAAATAAACTG
  AAAGATATCAGCACCTTGGAACCTTTGAAAAAGTTAGAATGTCTGAAAAGCCTGGACCTC
  TTTAACTGTGAGGTTACCAACCTGAATGACTACCGAGAGAGTGTCTTCAAGCTCCTGCCC
  CAGCTTACCTACTTGGATGGCTATGACCGAGAGGACCAGGAAGCACCTGACTCAGATGCC
  GAGGTGGATGGTGTGGATGAAGAGGAGGAGGACGAAGAAGGAGAAGATGAGGAAGACGAG
  GACGATGAGGATGGTGAAGAAGAGGAGTTTGATGAAGAAGATGATGAAGATGAAGATGTA
  GAAGGGGATGAGGACGACGATGAAGTCAGTGAGGAGGAAGAAGAATTTGGACTTGATGAA
  GAAGATGAAGATGAGGATGAGGATGAAGAGGAGGAAGAAGGTGGGAAAGGTGAAAAGAGG
  AAGAGAGAAACAGATGATGAAGGAGAAGATGATTAA
  
  Clone variation with respect to NM_006401.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006401 unedited
  TAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTAACTTGGCTCCGGGGG
  CTCCGCTCGCCTGCCCGCACGCCGCCCGCCACCCAGGACCGCGCCGCCGGCCTCCGCCGC
  TAGCAAACCCTTCCGACGGCCCTCGCTGCGCAAGCCGGGACGCCTCTCCCCCCTCCGCCC
  CCGCCGCGGAAAGTTAAGTTTGAAGAGGGGGGAAGAGGGGAACATGGACATGAAGAGGAG
  GATCCACCTGGAGCTGAGGAACCGGACCCCGGCAGCTGTTCGAGAACTTGTCTTGGACAA
  TTGCAAATCAAATGATGGAAAAATTGAGGGCTTAACAGCTGAATTTGTGAACTTAGAGTT
  CCTCAGTTTAATAAATGTAGGCTTGATCTCAGTTTCAAATCTCCCCAAGCTGCCTAAATT
  GAAAAAGCTTGAACTCAGTGAAAATAGAATCTTTGGAGGTCTGGACATGTTAGCTGAAAA
  ACTTCCAAATCTCACACATCTAAACTTAAGTGGAAATAAACTGAAAGATATCAGCACCTT
  GGAACCTTTGAAAAAGTTAGAATGTCTGAAAAGCCTGGACCTCTTTAACTGTGAGGTTAC
  CAACCTGAATGACTACCGAGAGAGTGTCTTCAAGCTCCTGCCCCAGCTTACCTACTTGGA
  TGGCTATGACCGAGAGGACCAGGAAGCACCTGACTCANNATGCCGAGTGGATGGTGTGGA
  TGAAGAGGAGGAGGACGAANAAGGAGAGATGAGGAAGACGAGGACGATGAGGATGGTGAG
  AANNNAAGAGTTGATGAAAAAGATGATGAANATGAGATGTNNAAGGGGATGAGGACACNA
  TGAAGTCGTGAGGGAGAAGAAGAATTTGACTTGTGAAAANATGANATGAGGTGAGGATGA
  AAGGAGGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006401 unedited
  TTTATGGACCCGCGGCCGCAATCTTNAGTCGAGTTTTTTTTTTTTTTTTTTAAATCCATG
  AGCAGTCCATCCAATACTGAACAGTTCTGTTTCTGCAGGTCATCTGGGGTCTTAATCATC
  TTCTCCTTCATCATCTGTTTCTCTCTTCCTCTTTTCACCTTTCCCACCTTCTTCCTCCTC
  TTCATCCTCATCCTCATCTTCATCTTCTTCATCAAGTCCAAATTCTTCTTCCTCCTCACT
  GACTTCATCGGCGTCCTCATCCCCTTCTACATCTTCATCTTCATCATCTTCTTCATCAAA
  CTCCTCTTCTTCACCATCCTCATCGTCCTCGTCTTCCTCATCTTCTCCTTCTTCGTCCTC
  CTCCTCTTCATCCACACCATCCACCTCGGCATCTGAGTCAGGTGCTTCCTGGTCCTCTCG
  GTCATAGCCATCCAAGTAGGTAAGCTGGGGCAGGAGCTTGAAGACACTCTCTCGGTAGTC
  ATTCAGGTTGGTAACCTCACAGTTAAAGAGGTCCAGGCTTTTCAGACATTCTAACTTTTT
  CAAAGGTTCCAAGGTGCTGATATCTTTCAGTTTATTTCCACTAAAGTTAGATGTGTGAGA
  TTTGGAAGTTTTCAGCTAACATGTCCAGACCTCCAAAGATCCTATTTTCACTGAGTTCCA
  GCTTTTTCATTTAAGCAGCCTGGGGGAGATTTGAAACTGAGATCAGCCCTACATTTATTA
  AACTGAGGAACTCCTAGGTCACAAATTCAGCTGTTAAGCCCTCAATTTTTTCATCATTTG
  ATTTGCAATTGTCCAAGAAAGTTTTCAACAGCTGCCGGGGTCGGGTCCCTCACTCCAGGT
  GGATCCTCCTCTAATGGGCATGTTCCCTTTTTCCCCCCCTTAAACTTAACTTTCCCNNNG
  GGGCCGAAGGGGAAAGCCCCCCCGTTTCCCAANAAGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006401
• Insert Size: 970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006401.1, NP_006392.1
• RefSeq Size: 1475 bp
• RefSeq ORF: 756 bp
• Locus ID: 10541
• UniProt ID: Q92688
• Cytogenetics: 9q22.33
• Domains: LRR, LRRcap
• Gene Summary: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.[UniProtKB/Swiss-Prot Function]