YKT6 (NM_006555) Human Untagged Clone

CAT#: SC116037

YKT6 (untagged)-Human YKT6 v-SNARE homolog (S. cerevisiae) (YKT6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YKT6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116037 sequence for NM_006555 edited (data generated by NextGen Sequencing)
  ATGAAGCTGTACAGCCTCAGCGTCCTCTACAAAGGCGAGGCCAAGGTGGTGCTGCTCAAA
  GCCGCATACGATGTGTCTTCCTTCAGCTTTTTCCAGAGATCCAGCGTTCAGGAATTCATG
  ACCTTCACGAGTCAACTGATTGTGGAGCGCTCATCGAAAGGCACTAGAGCTTCTGTCAAA
  GAACAAGACTATCTGTGCCACGTCTACGTCCGGAATGATAGTCTTGCAGGTGTGGTCATT
  GCTGACAATGAATACCCATCCCGGGTGGCCTTTACCTTGCTGGAGAAGGTACTAGATGAA
  TTCTCCAAGCAAGTCGACAGGATAGACTGGCCAGTAGGATCCCCTGCTACAATCCATTAC
  CCAGCCCTGGATGGTCACCTCAGTAGATACCAGAACCCACGAGAAGCTGATCCCATGACT
  AAAGTGCAGGCCGAACTAGATGAGACCAAAATCATTCTGCACAACACCATGGAGTCTCTG
  TTAGAGCGAGGTGAGAAGCTAGATGACTTGGTGTCCAAATCCGAGGTGCTGGGAACACAG
  TCTAAAGCCTTCTATAAAACTGCCCGGAAACAAAACTCATGCTGTGCCATCATGTGA
  
  Clone variation with respect to NM_006555.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006555 unedited
  ATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCGGTGGCCCCGT
  CAGCAGCCGGCTGCTGAGAGGCCGGTAGGCGGCGGCGGTCCCGAGGGGCGGCGGCCGCGC
  TGCTCCCTGAGAACGGGTCCCGCAGCTGGGCAGGCGGGCGGCCTGAGGGCGCGGAGCCAT
  GAAGCTGTACAGCCTCAGCGTCCTCTACAAAGGCGAGGCCAAGGTGGTGCTGCTCAAAGC
  CGCATACGATGTGTCTTCCTTCAGCTTTTTCCAGAGATCCAGCGTTCAGGAATTCATGAC
  CTTCACGAGTCAACTGATTGTGGAGCGCTCATCGAAAGGCACTAGAGCTTCTGTCAAAGA
  ACAAGACTATCTGTGCCACGTCTACGTCCGGAATGATAGTCTTGCAGGTGTGGTCATTGC
  TGACAATGAATACCCATCCCGGGTGGCCTTTACCTTGCTGGAGAAGGTACTAGATGAATT
  CTCCAAGCAAGTCGACAGGATAGACTGGCCAGTAGGATCCCCTGCTACAATCCATTACCC
  AGCCCTGGATGGTCACCTCAGTAGATACCAGAACCCACGAGAAGCTGATCCCATGACTAA
  AGTGCAGGCCGAACTAGATGAGACCAAAATCATTCTGCACAACACCATGGAGTCTCTGTT
  AGAGCGAGGTGAGAAGCTAGATGACTTGGTGTCCAAATCCGAGGTGCTGGGAACACAGTC
  TAAAGCCTTCTATAAAACTGCCCGGAAACAAAACTCATGCTGTGCCATCATGTGATGCAG
  CCTGCCAGAAGCCCAATGCTGGAATGGCACCATCATTCACATCAGAACTGCAGCCCCTGG
  GAAAGAAGAGACAGCCATAGACGAGGAGCCAGAGTGGGGGCAGACTGGCCATTNNTATTN
  TGAAAGTCCTGCGAAAATGGATGGTGGAAGGNTGCNAAATGTCAAATCATATGTGTGTAG
  TGATTCTTGGAAGAATTGAGGTCCCAAGGTGNATTTTGGNCAATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006555 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATTTCAAAACAAC
  TTTATCCAGACAGGCGCCTCTCAAATAGAACACAGGGAAGTTAGGCAGCAGTTACTAAAA
  TACAGTCTCGCCAAATGATTTACAACAGAACACAACAGGAGCAGGGGATCTGTGGGTGGG
  GCTGGGCTGGGCCCTCTATCTCACAGGGCCTGAGTCAAGCCAGCCCGCCCTGCAAGGCAG
  GGGCTGACCTGCAAGCGGAGATCTCACTTCCTCTTACCCCAAATTCATACCTCCATTTTC
  CCCGCCCCCATCTCTCCCCAGGGTCCTCAAGTGGGAAAGGGAGAGGTAGCATCCCTCGGA
  TCCAGGCCCACTCCACTCCGTCTCCGGCACCAGTGGGCAGGCTGAGTCTGGGCCTCAAGG
  GGCCCTGGGCTTAGGGTATCTATGGCAGTAGGAAAATGACATGGACAGGCTCTTCAGGGG
  TAGGCTAAAGTCCTCTGGCCAGCAGTACCCAGAGAAAATGGGCAGCAGCAGGTAAACCAG
  CCAGGAGGTGGAGTCCTCTGAACCCACAGCAGACCCCACCCTCCTGCCCAGCCCCTGCCC
  ACATTGGGGGTCAGGACCACTGAGACTCTGGTCAGGACAGTGGGTGCTCTCAGCAGTGTG
  GCAAGCTCAGAGCAGAGCTCCCAAGGACCATACCACACTGGTTCANAACCCATAGGTGAC
  ACCATCCCAGCAGAAGCTTCCATGGGTGCTGGATCCCCAGGCTGCATCCTGAGCACAGGT
  GGGCAGACTGGAAACATACACTANGACCCNAGGGATCCAGACATTTTANGCCCATCTCCT
  GGGCTGCTNCAGCCTGTTGCCATGACTTTGGCAGTGAGTGGGCCCTCCTGCCAGTGGCAG
  GNCACAGCTTTAAACCAAACCCTTGGNCTNCCCNCTCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006555
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006555.3, NP_006546.1
• RefSeq Size: 2783 bp
• RefSeq ORF: 597 bp
• Locus ID: 10652
• UniProt ID: O15498
• Cytogenetics: 7p13
• Domains: synaptobrevin
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: This gene product is one of the SNARE recognition molecules implicated in vesicular transport between secretory compartments. It is a membrane associated, isoprenylated protein that functions at the endoplasmic reticulum-Golgi transport step. This protein is highly conserved from yeast to human and can functionally complement the loss of the yeast homolog in the yeast secretory pathway. [provided by RefSeq, Jul 2008]