SC5DL (SC5D) (NM_006918) Human Untagged Clone

CAT#: SC115783

SC5DL (untagged)-Human sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (SC5DL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SC5DL
• Synonyms: ERG3; S5DES; SC5DL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115783 sequence for NM_006918 edited (data generated by NextGen Sequencing)
  ATGGATCTTGTACTCCGTGTTGCAGATTACTATTTTTTTACACCATACGTGTATCCAGCC
  ACATGGCCAGAAGATGACATCTTCCGACAAGCTATTAGTCTTCTGATTGTAACAAATGTT
  GGTGCTTACATCCTTTATTTCTTCTGTGCAACACTGAGCTATTATTTTGTCTTCGATCAT
  GCATTAATGAAACATCCACAATTTTTAAAGAATCAAGTCCGTCGAGAGATTAAGTTTACT
  GTCCAGGCATTGCCATGGATAAGTATTCTTACTGTTGCACTGTTCTTGCTGGAGATAAGA
  GGTTACAGCAAATTACATGATGACCTAGGAGAGTTTCCATATGGATTGTTTGAACTTGTC
  GTTAGTATAATATCTTTCCTCTTTTTCACTGACATGTTCATCTACTGGATTCACAGAGGC
  CTTCATCATAGACTGGTATATAAGCGCCTACATAAACCTCACCATATTTGGAAGATTCCT
  ACTCCATTTGCAAGTCATGCTTTTCACCCTATTGATGGCTTTCTTCAGAGTCTACCTTAC
  CATATATACCCTTTTATCTTTCCATTACACAAGGTGGTTTATTTAAGTCTGTACATCTTG
  GTTAATATCTGGACAATTTCCATTCATGACGGTGATTTTCGTGTCCCCCAAATCTTACAG
  CCATTTATTAATGGCTCAGCTCATCATACAGACCACCATATGTTCTTTGACTATAATTAT
  GGACAATATTTCACTTTGTGGGATAGGATTGGCGGCTCATTCAAAAATCCTTCATCCTTT
  GAGGGGAAGGGACCGCTCAGTTATGTGAAGGAGATGACAGAGGGAAAGCGCAGCAGCCAT
  TCAGGAAATGGCTGTAAGAATGAAAAATTATTCAATGGAGAGTTTACAAAGACTGAATAG
  
  Clone variation with respect to NM_006918.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006918 unedited
  GTTTGGATTTTGTAATCCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGGGC
  GTGCGGAGCGGCGGCGGACCACCTCCAGGGCTAAGTGATGGATCTTGTACTCCGTGTTGC
  AGATTACTATTTTTTTACACCATACGTGTATCCAGCCACATGGCCAGAAGATGACATCTT
  CCGACAAGCTATTAGTCTTCTGATTGTAACAAATGTTGGTGCTTACATCCTTTATTTCTT
  CTGTGCAACACTGAGCTATTATTTTGTCTTCGATCATGCATTAATGAAACATCCACAATT
  TTTAAAGAATCAAGTCCGTCGAGAGATTAAGTTTACTGTCCAGGCATTGCCATGGATAAG
  TATTCTTACTGTTGCACTGTTCTTGCTGGAGATAAGAGGTTACAGCAAATTACATGATGA
  CCTAGGAGAGTTTCCATATGGATTGTTTGAACTTGTCGTTAGTATAATATCTTTCCTCTT
  TTTCACTGACATGTTCATCTACTGGATTCACAGAGGCCTTCATCATAGACTGGTATATAA
  GCGCCTACATAAACCTCACCATATTTGGAAGATTCCTACTCCATTTGCAAGTCATGCTTT
  TCACCCTATTGATGGCTTTCTTCAGAGTCTACCTTACCATATATACCCTTTTATCTTTCC
  ATTACACAAGGTGGTTTATTTAAGTCTGTACATCTTGGTTAATATCTGGACAATTTCCAT
  TCATGACGGTGNATTTCGTGTCCCCCATATCTTACAGCCATTTATTAATGGCTCAGCTCA
  TCATACAGACCACCATATGTTCTTTGACTATNATTATGGNACATATTTCACTTTGTGGGG
  ATAGATGGNNCGCTCATNCAAAATCCNTCATCCTTNGAGGAGAAGGNACCGCTCANNTAT
  GTGAAAGAGATGACAGAGGGAATGCGCAGCACCATTCAGGAATGGCTGTAGAATGAAAAC
  TAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006918 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTAATTATGATCAACTT
  TTATTGATTTTACATAAATAGCTTATAGAATGCCTTTACAAACTATGTTTTTGCAATATA
  TTTGTTACAACACACATTTCCTTATGAATGTATTTGTTAAATCTTTTTGCAGAGGAAGTA
  GGGAAAAATGAGGGGAGAAGAGGAGGAATAAGATTTTAGAACTAATCGTCAGCAATGGGC
  CTAGGTATTTGGCAATTATTTCCTTTACAAAAAAATTACAGGTTTGTAACTTCAAACATC
  AAAAGTGTAGTGCATCAATTTAACTAAAAAATCAGTAGTGCCTAATATATTATCTGGGCA
  TCAAATTTCTTTTTTTAAATATATCCAGATTCACATATTTTTACTCTTATTAAATAATGG
  TTTTAAATAATAATCATCACAATGTTTAGCTACATAATCCCAGATCTGGATTAAATCATG
  GTGATATCGATATTTTTAAATTCCAATTTATTTGTTAATCAGTAAAATGTAATAGGTGAT
  AGTGTGTCCAGAGAATGAGCCTCCTATGTGTAGGAAGACGATAGTCTTATTACACCTGCT
  ATCAATTAGGTCATTTACATAGCTGCTAATGTATCAGAATTTTTTTCCACACTGATGTAG
  TACTTTATTCAAGTCAAAACAAGTTATCATTATCATACCAACGTTGAAGAATTAACCATT
  CCTGAAACAAGAAACAATCTTTACATTAGTATTTCTTGGTTATGGGGCTTAGTCTTGGTA
  TTATTAAGCACAATGATTCTTAATAAACATGGNGTGGATGANAGGCAATGCATATAGCCT
  ANTCCCCCTCATATTTATTCTNCATCTTATTTAAAATGNTGNTGAGGGCTATACAACTAC
  AGATCTGCTGCTAGAAAGAACAAATTGTCCCCGGACACATCTGCATTTTAGCATAGATTA
  GNNCTGGNGTCCAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006918
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006918.3, NP_008849.2
• RefSeq Size: 2164 bp
• RefSeq ORF: 900 bp
• Locus ID: 6309
• UniProt ID: O75845
• Cytogenetics: 11q23.3-q24.1
• Domains: Sterol_desat
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: This gene encodes an enzyme of cholesterol biosynthesis. The encoded protein catalyzes the conversion of lathosterol into 7-dehydrocholesterol. Mutations in this gene have been associated with lathosterolosis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein.