Coronin 1a (CORO1A) (NM_007074) Human Untagged Clone

CAT#: SC115708

CORO1A (untagged)-Human coronin, actin binding protein, 1A (CORO1A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Coronin 1a
• Synonyms: CLABP; CLIPINA; HCORO1; IMD8; p57; TACO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115708 sequence for NM_007074 edited (data generated by NextGen Sequencing)
  ATGAGCCGGCAGGTGGTCCGCTCCAGCAAGTTCCGCCACGTGTTTGGACAGCCGGCCAAG
  GCCGACCAGTGCTATGAAGATGTGCGCGTCTCACAGACCACCTGGGACAGTGGCTTCTGT
  GCTGTCAACCCTAAGTTTGTGGCCCTGATCTGTGAGGCCAGCGGGGGAGGGGCCTTCCTG
  GTGCTGCCCCTGGGCAAGACTGGACGTGTGGACAAGAATGCGCCCACGGTCTGTGGCCAC
  ACAGCCCCTGTGCTAGACATCGCCTGGTGCCCGCACAATGACAACGTCATTGCCAGTGGC
  TCCGAGGACTGCACAGTCATGGTGTGGGAGATCCCAGATGGGGGCCTGATGCTGCCCCTG
  CGGGAGCCCGTCGTCACCCTGGAGGGCCACACCAAGCGTGTGGGCATTGTGGCCTGGCAC
  ACCACAGCCCAGAACGTGCTGCTCAGTGCAGGTTGTGACAACGTGATCATGGTGTGGGAC
  GTGGGCACTGGGGCGGCCATGCTGACACTGGGCCCAGAGGTGCACCCAGACACGATCTAC
  AGTGTGGACTGGAGCCGAGATGGAGGCCTCATTTGTACCTCCTGCCGTGACAAGCGCGTG
  CGCATCATCGAGCCCCGCAAAGGCACTGTCGTAGCTGAGAAGGACCGTCCCCACGAGGGG
  ACCCGGCCCGTGCGTGCAGTGTTCGTGTCGGAGGGGAAGATCCTGACCACGGGCTTCAGC
  CGCATGAGTGAGCGGCAGGTGGCGCTGTGGGACACAAAGCACCTGGAGGAGCCGCTGTCC
  CTGCAGGAGCTGGACACCAGCAGCGGTGTCCTGCTGCCCTTCTTTGACCCTGACACCAAC
  ATCGTCTACCTCTGTGGCAAGGGTGACAGCTCAATCCGGTACTTTGAGATCACTTCCGAG
  GCCCCTTTCCTGCACTATCTCTCCATGTTCAGTTCCAAGGAGTCCCAGCGGGGCATGGGC
  TACATGCCCAAACGTGGCCTGGAGGTGAACAAGTGTGAGATCGCCAGGTTCTACAAGCTG
  CACGAGCGGAGGTGTGAGCCCATTGCCATGACAGTGCCTCGAAAGTCGGACCTGTTCCAG
  GAGGACCTGTACCCACCCACCGCAGGGCCCGACCCTGCCCTCACGGCTGAGGAGTGGCTG
  GGGGGTCGGGATGCTGGGCCCCTCCTCATCTCCCTCAAGGATGGCTACGTACCCCCAAAG
  AGCCGGGAGCTGAGGGTCAACCGGGGCCTGGACACCGGGCGCAGGAGGGCAGCACCAGAG
  GCCAGTGGCACTCCCAGCTCGGATGCCGTGTCTCGGCTGGAGGAGGAGATGCGGAAGCTC
  CAGGCCACGGTGCAGGAGCTCCAGAAGCGCTTGGACAGGCTGGAGGAGACAGTCCAGGCC
  AAGTAG
  
  Clone variation with respect to NM_007074.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007074 unedited
  GTTACNATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTCCTCCTCC
  TCCACCTCCGGCTTTTGGGGGATCACTGTCCTCTCTCGGCAGCAGAATGAGCCGGCAGGT
  GGTCCGCTCCAGCAAGTTCCGCCACGTGTTTGGACAGCCGGCCAAGGCCGACCAGTGCTA
  TGAAGATGTGCGCGTCTCACAGACCACCTGGGACAGTGGCTTCTGTGCTGTCAACCCTAA
  GTTTGTGGCCCTGATCTGTGAGGCCAGCGGGGGAGGGGCCTTCCTGGTGCTGCCCCTGGG
  CAAGACTGGACGTGTGGACAAGAATGCGCCCACGGTCTGTGGCCACACAGCCCCTGTGCT
  AGACATCGCCTGGTGCCCGCACAATGACAACGTCATTGCCAGTGGCTCCGAGGACTGCAC
  AGTCATGGTGTGGGAGATCCCAGATGGGGGCCTGATGCTGCCCCTGCGGGAGCCCGTCGT
  CACCCTGGAGGGCCACACCAAGCGTGTGGGCATTGTGGCCTGGCACACCACAGCCCAGAA
  CGTGCTGCTCAGTGCAGGTTGTGACAACGTGATCATGGTGTGGGACGTGGGCACTGGGGC
  GGCCATGCTGACACTGGGCCCAGAGGTGCACCCAGACACGATCTACAGTGTGGACTGGAG
  CCGAGATGGAGGCCTCATTTGTACCTCCTGCCGTGACAAGCGCGTGCGCATCATCGAGCC
  CCGCANAGCACTGTCGTAGCTGAGAGGACCGTCCCCACGAGGGGACCCGCCCGTGCGTGC
  AGTGTTCGTGTTGGAAGGGGAGATTCTGACCACGGCCTTTCACCCCCTGAATGAGCCGCA
  CNTGGCGCCTGTGGGACACAAAGCACCTGGNAGGAGCCGTTGTCCCTGCAAGAACTTGTA
  ACCCACAACGGGGTTCTGCTTGCCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007074 unedited
  AATTCCTGCNACCGCGGCCGCAATTTTANAGTCGGTTTTTTTTTTTTTTTTTTTACCAGA
  AAAAAAGCTATTTTATTATGATTTTTTTCTCTGCCATGTGGCTGGGAATGGGAGGTGAGT
  GGATGGGTGTGAATGGCTGACCCTGCTGGAGGCCCTGCGGGGCTCTACTTGGCCTGGACT
  GTCTCCTCCAGCCTGTCCAAGCGCTTCTGGAGCTCCTGCACCGTGGCCTGGAGCTTCCGC
  ATCTCCTCCTCCAGCCGAGACACGGCATCCGAGCTGGGAGTGCCACTGGCCTCTGGTGCT
  GCCCTCCTGCGCCCGGTGTCCAGGCCCCGGTTGACCCTCAGCTCCCGGTTCTTTGGGGGT
  ACGTACCCATCCTTGAGGGAGATGAGGAGGGGCCCAGCATCCCGACCCCCCAGCCACTCC
  TCAGCCGTGAGGGCAGGGTCGGGCCCTGCGGTGGGTGGGTACAGGTCCTCCTGGAACAGG
  TCCGACTTTCGAGGCACTGTCATGGCAATGGGCTCACACCTCCCCTCGTGCAGCTTGTAC
  AACCCTGGCGATCTCCCACTTGCTCACCTCCAGGTCACGTTTTGACCATGCAGCCTATGC
  CCCCGTTGGGACCTCTTTGAACCTGACCATGCAAAGATAGTGCCGGAAAGGGGGCCCCCG
  GCAGTGATCTTTCAAGCCCCGGTACTGATCCCGCCATCCTTTGCCACACACGGTACACTA
  TGCTTGCCGCTCTCTGGTTAAACAAGCGCCCACACGGTAACCCGCTTCCAGTGCGCCTAA
  GCTTTCTTGCTAGGCACCCGTGCGTTCCTCTTCGCGGCTTCTCTCCTTCCACATTGGTAA
  CCCTCGCAGCTTTCCTCTTTTGCGCCTGTAACTCCCGTTGTCACTCCATATTTTTCCTCC
  TCATCTCCCCACCCTTGCACCCCCCCGCACCCGTTCTCCCTCCTGCCCCTTCATTCTTTT
  CCACACCCCACTTTCAGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007074
• Insert Size: 1610 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007074.2, NP_009005.1
• RefSeq Size: 1620 bp
• RefSeq ORF: 1386 bp
• Locus ID: 11151
• UniProt ID: P31146
• Cytogenetics: 16p11.2
• Domains: WD40
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.