POMT1 (NM_007171) Human Untagged Clone

CAT#: SC115661

POMT1 (untagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POMT1
• Synonyms: LGMD2K; LGMDR11; MDDGA1; MDDGB1; MDDGC1; RT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007171, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGGGATTTTTGAAGCGCCCTGTAGTGGTGACGGCTGACATCAACTTGAGCCTTGTGGCCCTGACTG
  GGATGGGGTTACTGAGCCGGCTGTGGCGACTCACCTACCCGCGGGCTGTGGTTTTTGACGAAGTATATTA
  TGGGCAGTACATCTCTTTTTACATGAAACAAATCTTCTTCTTGGATGACAGTGGGCCGCCATTTGGCCAC
  ATGGTGCTGGCCTTGGGAGGTTATTTAGGAGGATTCGATGGCAATTTTTTGTGGAACAGAATTGGAGCAG
  AATACAGTAGCAACGTGCCTGTGTGGTCCCTGCGCCTGCTGCCAGCACTCGCGGGGGCCTTGTCGGTCCC
  CATGGCCTACCAGATAGTGTTGGAGCTCCACTTTTCTCATTGTGCCGCCATGGGAGCTGCTCTGTTGATG
  CTTATCGAGAATGCTCTCATCACTCAGTCAAGGCTAATGCTTTTGGAATCAGTGTTAATATTTTTCAATC
  TATTGGCCGTGTTGTCCTACCTGAAGTTCTTCAACTGCCAAAAGCACAGCCCTTTTTCTCTGAGCTGGTG
  GTTCTGGCTAACACTGACAGGGGTCGCTTGTTCCTGTGCAGTGGGCATCAAGTACATGGGTGTGTTCACG
  TACGTGCTCGTGCTGGGTGTTGCAGCTGTCCATGCCTGGCACCTGCTTGGAGACCAGACTTTGTCCAATG
  TAGGTGCTGATGTCCAGTGCTGCATGAGGCCGGCCTGTATGGGGCAGATGCGGATGTCACAGGGGGTCTG
  TGTGTTCTGTCACTTGCTCGCCCGAGCAGTGGCTTTGCTGGTCATCCCGGTCGTCCTGTACTTACTGTTC
  TTCTACGTCCACTTGATTCTAGTCTTCCGCTCTGGGCCCCACGACCAAATCATGTCCAGTGCCTTCCAGG
  CCAGCTTAGAGGGAGGACTAGCTCGGATCACCCAGGGTCAGCCACTGGAGGTGGCCTTTGGGTCCCAGGT
  CACTCTGAGGAACGTCTTTGGGAAACCTGTGCCCTGCTGGCTTCATTCCCACCAGGACACCTACCCCATG
  ATATATGAGAACGGCCGAGGCAGCTCCCACCAGCAACAGGTGACCTGTTACCCCTTCAAAGACGTCAATA
  ACTGGTGGATTGTAAAGGATCCCAGGAGGCACCAGCTGGTGGTGAGCAGCCCTCCGAGACCTGTGAGGCA
  CGGGGACATGGTGCAGCTGGTCCACGGCATGACCACCCGCTCCCTGAACACGCATGATGTTGCAGCCCCC
  CTGAGCCCCCATTCACAGGAGGTCTCCTGCTACATTGACTATAACATCTCCATGCCCGCCCAGAACCTCT
  GGAGACTGGAAATTGTGAACAGAGGATCTGACACAGACGTCTGGAAGACCATCCTCTCAGAGGTCCGCTT
  TGTGCACGTGAACACTTCCGCTGTCTTAAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTATCGGCAA
  CTGGAGATCGTCGGGGAGAAGCTGTCCCGGGGCTACCACGGGAGCACGGTGTGGAACGTGGAGGAGCACC
  GATACGGCGCGAGCCAGGAGCAGAGGGAGCGGGAACGGGAGCTGCACTCACCTGCGCAGGTGGACGTCAG
  CAGGAACCTCAGCTTCATGGCGAGATTCTCGGAGCTGCAGTGGAGGATGCTGGCGCTGAGAAGTGATGAC
  TCGGAACACAAGTACAGCTCCAGCCCACTGGAGTGGGTCACCCTGGACACCAATATTGCCTACTGGCTGC
  ACCCCAGGACCAGCGCTCAGATCCACCTACTTGGAAACATAGTGATCTGGGTTTCGGGCAGCCTCGCTCT
  GGCCATCTACGCCCTGCTGTCCTTGTGGTACCTGCTCCGACGGCGAAGAAATGTCCATGACCTCCCTCAG
  GATGCCTGGCTGCGCTGGGTGCTGGCTGGGGCGCTGTGTGCCGGTGGCTGGGCAGTGAACTACCTCCCGT
  TCTTCCTGATGGAGAAGACACTCTTCCTCTACCACTACCTGCCCGCACTCACCTTCCAAATCCTTCTGCT
  CCCTGTGGTCCTGCAGCACATCAGCGACCACCTGTGCAGGTCCCAGCTCCAGAGGAGCATCTTCAGCGCC
  CTGGTGGTGGCCTGGTACTCCTCCGCGTGCCACGTGTCCAACACGCTGCGCCCACTCACCTACGGGGACA
  AGTCACTCTCGCCACATGAACTCAAGGCCCTTCGCTGGAAAGACAGCTGGGACATCTTGATCCGAAAACA
  CTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007171 unedited
  GTCGNNAATTGTTAACACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGCGGAGTGCCG
  AGCGGCCTCACCCCCAACCGTCGGCCCAGTCGGACGGTTCCGAGGCGTTGCCGGGAGCCG
  GGCGCGGCTCTGTGTGGACTCGGAGAAACGCGGGGCGTCTGCCTGAGCCCGCTTTTCTAC
  AAGATGTGGGGATTTTTGAAGCGCCCTGTAGTGGTGACGGCTGACATCAACTTGAGCCTT
  GTGGCCCTGACTGGGATGGGGTTACTGAGCCGGCTGTGGCGACTCACCTACCCGCGGGCT
  GTGGTTTTTGACGAAGTATATTATGGGCAGTACATCTCTTTTTACATGAAACAAATCTTC
  TTCTTGGATGACAGTGGGCCGCCATTTGGCCACATGGTGCTGGCCTTGGGAGGTTATTTA
  GGAGGATTCGATGGCAATTTTTTGTGGAACAGAATTGGAGCAGAATACAGTAGCAACGTG
  CCTGTGTGGTCCCTGCGCCTGCTGCCAGCACTCGCGGGGGCCTTGTCGGTCCCCATGGCC
  TACCAGATAGTGTTGGAGCTCCACTTTTCTCATTGTGCCGCCATGGGAGCTGCTCTGTTG
  ATGCTTATCGAGAATGCTCTCATCACTCAGTCAAGGCTAATGCTTTTGGAATCAGTGTTA
  ATATTTTTCAATCTATTGGCCGTGTTGTCCTACCTGAAGTTCTTCAACTGCCAAAAGCAC
  AGCCCTTTTTCTCTGAGCTGGTGGTTCTGGGCTACACTGACAGGNGTCGCTTGTTCCTGT
  GCAGTGGGCATCAAGTACATGGGTGTGTTCACGTACGTGCTCGTGCTGGGTGTTGCAGCT
  GTTCATGCCTGGCACCTGCTTGGAGACAGACTTTGTCCATGTCTGTGTGTTCTGTCACTG
  CTCGCCCAGCGTGGCTTGCTGTCATCCGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007171 unedited
  NTTGCTCTGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTGCCAGTTGGTTCCTTTTTTAATTACATTTAAAAAAGACAAAGGCATGGGAGCT
  GTATGGATGACACACCTTTGACCACTGACGAGGGAAGATTTCACTCAGCCACGTCTCAGT
  GGAGTCTCAAAGGGGAGGGTTTCCACACAGCACGGTGCCAAGGGCCAGTCTTGACGCTGT
  CATAAACAATGACATGCAAGCCCCAGCCCTGGCTCGTGGTTCAGGTGGCCCTCCAACACT
  GAGCTGTCCCAGGACGCCTGCCGGGCTCGCTGAGGGCAGGGCCGGGACTTGCTCAGGTTG
  CTGGGTGTTCATGAAGGAGGATCGGGTCTGCCAGATCAAACTCCACCCTGCAGCAGTTAG
  CAAGGACCAGGCCCTGGTCTGCTGAGGCTCCACTGCTGGCCCTGGGTTCTCGGCTTTTTC
  GTGCAATGACTCCTACGCTGGCCTCTGAAATACTCCCTGTGTCTTAGTTCAGGGGTAAAG
  ACACCGGATATCTTTATTGTCGAAAAAAATTAACTTTGACAAAAAGAAGTGCTCATTAAA
  AGAGCTTTTCAATGAGACCCCCATGTGTTTCCACTAAAGGCCCTGTTTAACCCAAACCCG
  TGCTCTCCCACCGGGGGGCCCCCCCTGTTAATTATGTCATTTGACCAAGAACCTTTTAAC
  TTTATTCCCGAACCCAAAAAGGGGGGTGTCTTTCGCCCACTCCTTGGTTTCTTAAGGGTT
  TTGGGAACCAAAAAAGTCCCCACTCTGCTTTTCCCACCGAGAGGCCCTCTGAATTTATTG
  GGGAGAAAAAGGACTTTTCCCCCTCATAGGAAAGGGCGCCACCCGTGTTGCACACCCTGG
  CACCCGCAGGGAAAACCAACCACCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007171
• Insert Size: 3240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007171.2, NP_009102.2
• RefSeq Size: 3146 bp
• RefSeq ORF: 2244 bp
• Locus ID: 10585
• UniProt ID: Q9Y6A1
• Cytogenetics: 9q34.13
• Domains: PMT, MIR
• Protein Families: Transmembrane
• Protein Pathways: O-Mannosyl glycan biosynthesis
• Gene Summary: The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 6 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.