MAFF (NM_012323) Human Untagged Clone

CAT#: SC115453

MAFF (untagged)-Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) (MAFF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAFF
• Synonyms: hMafF; U-MAF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012323, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGTGGATCCCCTATCCAGCAAAGCTCTAAAGATCAAGCGAGAGCTGAGCGAGAACACGCCGCACC
  TGTCGGACGAGGCGCTGATGGGGCTGTCGGTGCGCGAGCTGAACCGGCATCTGCGCGGGCTCTCCGCCGA
  GGAGGTGACACGGCTCAAGCAGCGGCGCCGCACACTCAAAAACCGTGGCTACGCCGCCAGCTGCCGCGTG
  AAGCGCGTGTGCCAGAAGGAGGAGCTGCAGAAGCAGAAGTCGGAGCTGGAGCGCGAGGTGGACAAGCTGG
  CGCGCGAGAACGCCGCCATGCGCCTGGAGCTCGACGCGCTGCGCGGCAAGTGCGAGGCGCTGCAGGGCTT
  CGCGCGCTCCGTGGCCGCCGCCCGCGGGCCCGCCACGCTCGTGGCGCCGGCCAGCGTCATCACCATCGTC
  AAGTCCACCCCGGGCTCGGGGTCTGGCCCCGCCCACGGCCCGGACCCCGCCCACGGCCCGGCCTCCTGCT
  CCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012323 unedited
  NCGTCACCATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCGACCTGC
  GGCTCAGAGCGGAGGGGAGACTGACCGGAGCGCGGATCGGGACAGCGGCCGGGACAGCGG
  CGAGACGCGCGTGTGTGAGCGCGCCGGACCAAGCGGGCCCAGAAGCGGGTCTGCAGCCCA
  GAGGGCACCTTCTGCAAACATGTCTGTGGATCCCCTATCCAGCAAAGCTCTAAAGATCAA
  GCGAGAGCTGAGCGAGAACACGCCGCACCTGTCGGACGAGGCGCTGATGGGGCTGTCGGT
  GCGCGAGCTGAACCGGCATCTGCGCGGGCTCTCCGCCGAGGAGGTGACACGGCTCAAGCA
  GCGGCGCCGCACACTCAAAAACCGTGGCTACGCCGCCAGCTGCCGCGTGAAGCGCGTGTG
  CCAGAAGGAGGAGCTGCAGAAGCAGAAGTCGGAGCTGGAGCGCGAGGTGGACAAGCTGGC
  GCGCGAGAACGCCGCCATGCGCCTGGAGCTCGACGCGCTGCGCGGCAAGTGCGAGGCGCT
  GCAGGGCTTCGCGCGCTCCGTGGCCGCCGCCCGCGGGCCCGCCACGCTCGTGGCGCCGGC
  CAGCGTCATCACCATCGTCAAGTCCACCCCGGGCTCGGGGGTCTGGCCCCGCCCACGGGC
  CGGACCCCGCCCACGGCCCGGCCTCCTGTNNCTAGTGCCCGCCCNCGCCATGCCTCAGCC
  ACGCCCCTCCGGCCTCAGCTCCCTCCCCAAAGTGCCTGAGCGCCGCCTCTGTGCCCAGGT
  CCCATTTCTCTGCAGCACTGGCCCCCTTGGTGCACACACATTCCCCTTCGTGGGGCCCTG
  TCTTNCTCTTTGCAGCCCCCAAACTGGNGACCGAATGACCCTGNGAAGGNNGAANNTGNN
  GTAGTNGNNGATGGGGGCANAGGTCTGGATCTGGGATCGNCCTTGGNNCTGAAGTTACCT
  TNNTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012323
• Insert Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012323.2, NP_036455.1
• RefSeq Size: 2382 bp
• RefSeq ORF: 495 bp
• Locus ID: 23764
• UniProt ID: Q9ULX9
• Cytogenetics: 22q13.1
• Domains: bZIP_Maf, BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Variants 1, 3, and 4 encode the same isoform (a).