PI 3 Kinase p85 alpha (PIK3R1) (NM_181523) Human Untagged Clone

CAT#: SC115320

PIK3R1 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PI 3 Kinase p85 alpha
• Synonyms: AGM7; GRB1; IMD36; p85; p85-ALPHA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_181523 edited
  ATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTATAAAAAGGAAAGAGAAGAA
  GATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAAAGGGTCCTTAGTAGCTCTT
  GGATTCAGTGATGGACAGGAAGCCAGGCCTGAAGAAATTGGCTGGTTAAATGGCTATAAT
  GAAACCACAGGGGAAAGGGGGGACTTTCCGGGAACTTATGTAGAATATATTGGAAGGAAA
  AAAATCTCGCCTCCCACACCAAAGCCCCGGCCACCTCGGCCTCTTCCTGTTGCACCAGGT
  TCTTCGAAAACTGAAGCAGATGTTGAACAACAAGCTTTGACTCTCCCGGATCTTGCAGAG
  CAGTTTGCCCCTCCTGACATTGCCCCGCCTCTTCTTATCAAGCTCGTGGAAGCCATTGAA
  AAGAAAGGTCTGGAATGTTCAACTCTATACAGAACACAGAGCTCCAGCAACCTGGCAGAA
  TTACGACAGCTTCTTGATTGTGATACACCCTCCGTGGACTTGGAAATGATCGATGTGCAC
  GTTTTGGCTGACGCTTTCAAACGCTATCTCCTGGACTTACCAAATCCTGTCATTCCAGCA
  GCCGTTTACAGTGAAATGATTTCTTTAGCTCCAGAAGTACAAAGCTCCGAAGAATATATT
  CAGCTATTGAAGAAGCTTATTAGGTCGCCTAGCATACCTCATCAGTATTGGCTTACGCTT
  CAGTATTTGTTAAAACATTTCTTCAAGCTCTCTCAAACCTCCAGCAAAAATCTGTTGAAT
  GCAAGAGTACTCTCTGAAATTTTCAGCCCTATGCTTTTCAGATTCTCAGCAGCCAGCTCT
  GATAATACTGAAAACCTCATAAAAGTTATAGAAATTTTAATCTCAACTGAATGGAATGAA
  CGACAGCCTGCACCAGCACTGCCTCCTAAACCACCAAAACCTACTACTGTAGCCAACAAC
  GGTATGAATAACAATATGTCCTTACAAGATGCTGAATGGTACTGGGGAGATATCTCGAGG
  GAAGAAGTGAATGAAAAACTTCGAGATACAGCAGACGGGACCTTTTTGGTACGAGATGCG
  TCTACTAAAATGCATGGTGATTATACTCTTACACTAAGGAAAGGGGGAAATAACAAATTA
  ATCAAAATATTTCATCGAGATGGGAAATATGGCTTCTCTGACCCATTAACCTTCAGTTCT
  GTGGTTGAATTAATAAACCACTACCGGAATGAATCTCTAGCTCAGTATAATCCCAAATTG
  GATGTGAAATTACTTTATCCAGTATCCAAATACCAACAGGATCAAGTTGTCAAAGAAGAT
  AATATTGAAGCTGTAGGGAAAAAATTACATGAATATAACACTCAGTTTCAAGAAAAAAGT
  CGAGAATATGATAGATTATATGAAGAATATACCCGCACATCCCAGGAAATCCAAATGAAA
  AGGACAGCTATTGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAGTGCCAGACC
  CAAGAGCGGTACAGCAAAGAATACATAGAAAAGTTTAAACGTGAAGGCAATGAGAAAGAA
  ATACAAAGGATTATGCATAATTATGATAAGTTGAAGTCTCGAATCAGTGAAATTATTGAC
  AGTAGAAGAAGATTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTATCGAGAAATTGAC
  AAACGTATGAACAGCATTAAACCAGACCTTATCCAGCTGAGAAAGACGAGAGACCAATAC
  TTGATGTGGTTGACTCAAAAAGGTGTTCGGCAAAAGAAGTTGAACGAGTGGTTGGGCAAT
  GAAAACACTGAAGACCAATATTCACTGGTGGAAGATGATGAAGATTTGCCCCATCATGAT
  GAGAAGACATGGAATGTTGGAAGCAGCAACCGAAACAAAGCTGAAAACCTGTTGCGAGGG
  AAGCGAGATGGCACTTTTCTTGTCCGGGAGAGCAGTAAACAGGGCTGCTATGCCTGCTCT
  GTAGTGGTGGACGGCGAAGTAAAGCATTGTGTCATAAACAAAACAGCAACTGGCTATGGC
  TTTGCCGAGCCCTATAACTTGTACAGCTCTCTGAAAGAACTGGTGCTACATTACCAACAC
  ACCTCCCTTGTGCAGCACAACGACTCCCTCAATGTCACACTAGCCTACCCAGTATATGCA
  CAGCAGAGGCGATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181523 unedited
  AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGGAGTTG
  GAGGAAGCAGCGGCAGCGGCGAGGGCGGCAGGCTAGCTGTCGGAGACGGCAAGCACGGAT
  CGGGCACGGCCGGCGGGTCGCAGCCGGGCTGGAGGCCGGTCGGAACCGAGCGGGCAGGAG
  GCCACCGCTGCAGCGCGGGCCCCGCAGAGGAAGGAAGCCGGCGGGCGGGGTAGGTGAAGC
  TCGTGTGTGGAGTGCCACGGTACAATCAGACGACAGATGGACAGTGTGACAAAAGTGTCA
  GAAAGGATTGGGCCTCGCTGTGAGAGTCAGCCTGGATTCAAAGTGTTGACAAGTTGCTGA
  AAAGGAAGCCAGTGAGAGGACTGTGGCACGCAGAGGAAGTGGAGCCCTGTCTTCGGTCAC
  ACCATTGATGGAGGACAGATGGACAGCCGTATGGCCAGTCACCTCTCCTCTTAAACCTTT
  GGAGAGTGGTCCTTTGTCCTCTGCTGGACACATAATAGGAATTCTAACACATTCTCTGAA
  TTCACTTTTCATAAAAACGTAAAATCAGACTGCTCTGTACAACCAGGCTCAACTGTTGCA
  TGGTAGCAGATTTGCAAACATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTA
  TAAAAAGGAAAGAGAAGAAGATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAA
  AGGGTCCTTAGTAGCTCTTGGATTCAGTGATGGACAGGAAGCCCAGCCTGTAGAANATGG
  CTGGTTAAATGGCTATAATGAAACCACAGGGGAAAGGGGGGACTNTCCCGGGACTTATGT
  AGGATATATTGGAAGGAAAAAAATCTCGCCTTCCACACCAAAGCCCCGGCCACCTCGGCT
  CTTNNCTGTGCACCAGTTCTTCGAAACTGAAGCAGATGTTGACAACAGCTTTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181523 unedited
  NNNTTTTACTTTGAACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAA
  ATATTTCATAGTTTTAATTCTGAAGCCATTTTTTTTTTTTACTGGCATCCTGTACATTTA
  CTTTTAAAAAAGGATAACAAAAATGAATATTAACAAAAATCCGGGACAACAATATTTTCA
  AGCAACAAAAACTGGGGTGGGGAAGCTTATTCTGAAGGTACATTTAAAACTGAAATAACA
  ACTTAATGAAAATTAAAAATTGCATAGCGCTGTGAATTTAGCCTTCAGCAAAACAAAACA
  GAAGCTATTTGGTATTGATACAAATCCATCTATTTGATAGTTAGTCATCCAATATTATGT
  ACATATTTTATATACTGAATGTCATTTTAAGTCCTGTTTTCCAAACTCCATTTTTCTGTT
  GCTGGGTTTTTGTTTTTTGACAAGTTAAACACTTTCTGGCACTTTCTATGACAGAATTTC
  TTCTGAACATACATGAACTGACATTCTCCCAAAGCGTCCCTTGTGAGTGGACGTGCCTTT
  CTGCTACATATCGTTCATTTGTTACAAAATGAAATAATCCACAGTGCGATGTGTCTGGGT
  CCACCGTGCACAGCAACATCCAGGCTAAACCAGGCTGGACCAAACCTTCTCAGGCCAGGG
  TTCTTNTGCCTCTCTCTCCTCCGCTCTCCAGAGTCCCAACTTTCAGCTGGTAAAGCTTCA
  GAAAGCATTAACAAGCACCATGGTTAAAGGCCTCTTTGTCCCTGCCGCAGAGATTTTGCA
  TTTCTTTTCTCTTTGNGTGNATGGGGGNTGGGGCTTTAAATAAACCAAAGAAAAAGAAAA
  GAAGGATAAGCACTCACTTAGAACGTGTGTCATCTACACCTTAGCTGAATGGCTTCCCTA
  CTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181523
• Insert Size: 3890 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181523.1, NP_852664.1
• RefSeq Size: 6453 bp
• RefSeq ORF: 2175 bp
• Locus ID: 5295
• UniProt ID: P27986
• Cytogenetics: 5q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.