ACAD8 (NM_014384) Human Untagged Clone

CAT#: SC115026

ACAD8 (untagged)-Human acyl-CoA dehydrogenase family, member 8 (ACAD8), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACAD8
• Synonyms: ACAD-8; ARC42; IBDH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014384, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGTGGAGCGGCTGCCGGCGTTTCGGGGCGCGCCTCGGCTGCCTGCCCGGCGGTCTCCGGGTCCTCG
  TCCAGACCGGCCACCGGAGCTTGACCTCCTGCATCGACCCTTCCATGGGACTTAATGAAGAGCAGAAAGA
  ATTTCAAAAAGTGGCCTTTGACTTTGCTGCCCGAGAGATGGCTCCAAATATGGCAGAGTGGGACCAGAAG
  GAGCTGTTCCCAGTGGATGTGATGCGGAAGGCAGCCCAGCTAGGCTTCGGAGGGGTCTACATACAAACAG
  ATGTGGGCGGGTCTGGGCTGTCACGTCTTGATACCTCTGTCATTTTTGAAGCCTTGGCTACAGGCTGCAC
  CAGCACCACAGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTCGGAAATGAGGAA
  CAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTACTGCCTCACTGAACCAG
  GAAGTGGGAGTGATGCTGCCTCTCTTCTGACCTCCGCTAAGAAACAGGGAGATCATTACATCCTCAATGG
  CTCCAAGGCCTTCATCAGTGGTGCTGGTGAGTCAGACATCTATGTGGTCATGTGCCGAACAGGAGGACCA
  GGCCCCAAGGGCATCTCATGCATAGTTGTTGAGAAGGGGACCCCTGGCCTCAGCTTTGGCAAGAAGGAGA
  AAAAGGTGGGGTGGAACTCCCAGCCAACACGAGCTGTGATCTTCGAAGACTGTGCTGTCCCTGTGGCCAA
  CAGAATTGGGAGCGAGGGGCAGGGCTTCCTCATTGCCGTGAGAGGACTGAACGGAGGGAGGATCAATATT
  GCTTCCTGCTCCCTGGGGGCTGCCCACGCCTCTGTCATCCTCACCCGAGACCACCTCAATGTCCGGAAGC
  AGTTTGGAGAGCCTCTGGCCAGTAACCAGTACTTGCAATTCACACTGGCTGATATGGCAACAAGGCTGGT
  GGCCGCGCGGCTGATGGTCCGCAATGCAGCAGTGGCTCTGCAGGAGGAGAGGAAGGATGCAGTGGCCTTG
  TGCTCCATGGCCAAGCTCTTTGCTACAGATGAATGCTTTGCCATCTGCAACCAGGCCTTGCAGATGCACG
  GGGGCTACGGCTACCTGAAGGATTACGCTGTTCAGCAGTACGTGCGGGACTCCAGGGTCCACCAGATTCT
  AGAAGGTAGCAATGAAGTGATGAGGATACTGATCTCTAGAAGCCTGCTTCAGGAGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014384 unedited
  NGGGTCACCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAGCTGC
  GGCGGCTATGCTGTGGAGCGGCTGCCGGCGTTTCGGGGCGCGCCTCGGCTGCCTGCCCGG
  CGGTCTCCGGGTCCTCGTCCAGACCGGCCACCGGAGCTTGACCTCCTGCATCGACCCTTC
  CATGGGACTTAATGAAGAGCAGAAAGAATTTCAAAAAGTGGCCTTTGACTTTGCTGCCCG
  AGAGATGGCTCCAAATATGGCAGAGTGGGACCAGAAGGAGCTGTTCCCAGTGGATGTGAT
  GCGGAAGGCAGCCCAGCTAGGCTTCGGAGGGGTCTACATACAAACAGATGTGGGCGGGTC
  TGGGCTGTCACGTCTTGATACCTCTGTCATTTTTGAAGCCTTGGCTACAGGCTGCACCAG
  CACCACAGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTCGGAAA
  TGAGGAACAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTA
  CTGCCTCACTGAACCAGGAAGTGGGAGTGATGCTGCCTCTCTTCTGACCTCCGCTAAGAA
  ACAGGGAGATCATTACATCCTCAATGGCTCCAAGGCCTTCATCAGTGGTGCTGGTGAGTC
  AGACATCTATGTGGTCATGTGCCGAACAGGAGGACCAGGCCCCAAGGGCATCTCATGCAT
  AGTTGTTGAGAAAGGGGACCCTGGCCTCAGCTTTGGCAAGAAGGAGAAAAAGGTGGGGTG
  GAACTCCCAGCCACACGAGCTGTGATCTTCGAAGACTGTGCTGTCCCTGTGGCCAACAGA
  ATTGNNGAGCGAGGGGCAGGGCTTTCTCATTGCCGTGAGAAGACTGAACGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014384 unedited
  GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTAAAAGCCAAATTGGAC
  GAGAGTTTTATTATTCCAAAATCCAGTTAAGTGTTTAATCAAATACAATTAAACATTTAA
  AATAAAAATCAACTATAGTAGAACCAGAGGTGGTGAAGGATAGATTTTATCAATGTGATT
  AAAAATATTTAAAAGGAGGAACAGCTTCAGAAAAGGAAACAAAAACACAAAAAAAATATT
  CAAAGTGTTTATCAGGAAAAGAAACACTGAATAGCACTCCTAGACCACAGGCTTCTTAAC
  ATGAAACCCAACGAATATTCAAAATTCAAAAGTCTCAGCCCTTGATATTGAGGCAGTCCA
  GAAGTAGAAATACACCCTAAATCACAGCTTAGGAGTAAGTTTCCAAATATCCATTTTATC
  ATGATTTACATTCAGAGGTGTGCTATCCAGAGAAACCTTCTGCATCTGTAGCTGTGGCAC
  AGAGAACGACACAGACAGGAGGGCATTCCTCCATTTCCCCTTCCCACACCTTTGCTCTAG
  AATCAGGAAGAGCATCAGTGAAAGTCCCCAGGCCCCTAGAGGAGGGATCCAGTGAACTAG
  TAAAATGCAGACTAATCAAGATAAAGTGGATCCAAGAAAGGACTTTCATGGTTTGACGGT
  GAATCTTCACTGGTCACCCTTCTGGCATTAGGAAAACAAGGTAGTATGTGTTCTTCTGAT
  GTTTCTCATCAGTCCAGCTCTTCCGGTTCCACTGGGGATTCTGCCCCAGTCCAAGACCCG
  ATGCTGGTGCCAACACACAGGGTGCANGTCCTGCCCTANAGGAGCTCACCCCTTGGGTCT
  AATCATGATTCCTTGGATAGAGCGGNCACATGGCACACTCACACTGACTCAGTCGACTGA
  CACCAGCCAGACAGTGTGGGTCTACTCTGAGCAGCTNTAGAGACAGATCTATACTCATGC
  TACTTAGATCGGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014384
• Insert Size: 2340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014384.1, NP_055199.1
• RefSeq Size: 2216 bp
• RefSeq ORF: 1248 bp
• Locus ID: 27034
• UniProt ID: Q9UKU7
• Cytogenetics: 11q25
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The encoded protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency.[provided by RefSeq, Nov 2009]