NEK6 (NM_014397) Human Untagged Clone

CAT#: SC115003

NEK6 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 6 (NEK6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK6
• Synonyms: SID6-1512
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014397 edited
  ATGGCAGGACAGCCCGGCCACATGCCCCATGGAGGGAGTTCCAACAACCTCTGCCACACC
  CTGGGGCCTGTGCATCCTCCTGACCCACAGAGGCATCCCAACACGCTGTCTTTTCGCTGC
  TCGCTGGCGGACTTCCAGATCGAAAAGAAGATAGGCCGAGGACAGTTCAGCGAGGTGTAC
  AAGGCCACCTGCCTGCTGGACAGGAAGACAGTGGCTCTGAAGAAGGTGCAGATCTTTGAG
  ATGATGGACGCCAAGGCGAGGCAGGACTGTGTCAAGGAGATCGGCCTCTTGAAGCAACTG
  AACCACCCAAATATCATCAAGTATTTGGACTCGTTTATCGAAGACAACGAGCTGAACATT
  GTGCTGGAGTTGGCTGACGCAGGGGACCTCTCGCAGATGATCAAGTACTTTAAGAAGCAG
  AAGCGGCTCATCCCGGAGAGGACAGTATGGAAGTACTTTGTGCAGCTGTGCAGCGCCGTG
  GAGCACATGCATTCACGCCGGGTGATGCACCGAGACATCAAGCCTGCCAACGTGTTCATC
  ACAGCCACGGGCGTCGTGAAGCTCGGTGACCTTGGTCTGGGCCGCTTCTTCAGCTCTGAG
  ACCACCGCAGCCCACTCCCTAGTGGGGACGCCCTACTACATGTCACCGGAGAGGATCCAT
  GAGAACGGCTACAACTTCAAGTCCGACATCTGGTCCTTGGGCTGTCTGCTGTACGAGATG
  GCAGCCCTCCAGAGCCCCTTCTATGGAGATAAGATGAATCTCTTCTCCCTGTGCCAGAAG
  ATCGAGCAGTGTGACTACCCCCCACTCCCCGGGGAGCACTACTCCGAGAAGTTACGAGAA
  CTGGTCAGCATGTGCATCTGCCCTGACCCCCACCAGAGACCTGACATCGGATACGTGCAC
  CAGGTGGCCAAGCAGATGCACATCTGGATGTCCAGCACCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014397 unedited
  ATTGTAAACCACTACTATAGGCGGCCGCGAATTCGGCACGAGGCGCAAACTCGTGTGGGA
  CGCACCGCTCCAGCCGCCCGCGGGCCAGCGCACCGGTCCCCCAGCGGCAGCCGAGCCCGC
  CCGCGCGCCGTTCGTGCCCTCGTGAGGCTGGCATGCAGGATGGCAGGACAGCCCGGCCAC
  ATGCCCCATGGAGGGAGTTCCAACAACCTCTGCCACACCCTGGGGCCTGTGCATCCTCCT
  GACCCACAGAGGCATCCCAACACGCTGTCTTTTCGCTGCTCGCTGGCGGACTTCCAGATC
  GAAAAGAAGATAGGCCGAGGACAGTTCAGCGAGGTGTACAAGGCCACCTGCCTGCTGGAC
  AGGAAGACAGTGGCTCTGAAGAAGGTGCAGATCTTTGAGATGATGGACGCCAAGGCGAGG
  CAGGACTGTGTCAAGGAGATCGGCCTCTTGAAGCAACTGAACCACCCAAATATCATCAAG
  TATTTGGACTCGTTTATCGAAGACAACGAGCTGAACATTGTGCTGGAGTTGGCTGACGCA
  GGGGACCTCTCGCAGATGATCAAGTACTTTAAGAAGCAGAAGCGGCTCATCCCGGAGAGG
  ACAGTATGGAAGTACTTTGTGCAGCTGTGCAGCGCCGTGGAGCACATGCATTCACGCCGG
  GTGATGCACCGAGACATCAAGCCTGCCAACGTGTTCATCACAGCCACGGNCGTCGTGAAG
  CTCGGTGACCTTGGTCTGGGCCGCTTNCTCAGCTCTGAGACCACCGCAGCCCACTCCCTA
  GTGGGNGACGCCCTACTACATGTCACCGGNAAAGGATCCATGAGAACNNNGCTACACTTC
  AAGTCCGACATCTGGTCCTTGGGCTGTCTGCTGTACAAGATGCANCCCTNCAAAGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014397 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAAAATCTTCATGAATTCTAAGTA
  ACTCCCAGTCACCCTGTTTTATTTCAACCATGGAGAAAAGTACAGAGGAAAGGCTGCATA
  TGGAGAGACTGTCGGGCTGACGGTGTCACAGCAGATCCGAGTCCACGTGTGGAAACAGCA
  GCCGCCCGGCCCTGGGTGTTTCCTCCAGGAAAGGCCTGGTCAGTGAATGCCTGCAGGCAG
  CAGGTGTCAGGAATCACCTGCCCGATGCCAGCGCTGCTCTTGTCTGGAGGGCCAGACTGT
  CATGAAGTCAGTGACGACAGCTCAGACAATTGGAAGGTTAACTTTACGTACAAAAACAAA
  ATTCAAATGATTTCCTTCAGAGGTGGAGTTTTCTTAGGTTCACATTTATAAATTAGTCTT
  CACAGTTAATCCTGTTTGGGAACAAGAAATAAGGAACACATTTCTGGTTTCCTACACATG
  ATACAGGTGTACATTAATTCTCGACTTCCGTGGTCCATGGACTTCAGTTTGTGAAGGGTG
  AGTGGCTACCATTTTTGGATCATCTACTTTTTGTATTAGCTAACTTGCATATTTTATTTC
  AAATTCTGTCTGTCAGCATTCCTGAAGGTAGATGATAGGTACTATTAATCTCATTGTACA
  GATGACGAAATGAAAGCTCANAACGGCTAAAAAACTTGCCCAAGCCCCAAGTCACGCTAA
  GAAGTTGCAGAACCAGAATTTGTAAAGGAACAGCCTTCGCTTTTCATATNCCAATGCTTC
  TTCCATGGAGGAAATGCTTACCCTGATCTGAATGAACCGCCGCTTCAGGAGGGACCAATT
  TCTGTACCAACAGGGTTCAGAAATATGCCACCCTGCTGCCAGAGAGCTCTGCTCCCAGCC
  TTTGCTGGACCTCTGCCAGGCGGCTCTATTGCCCTCACAGTTTAACGGGAAAATGGTCCC
  AACCCCCCCCGGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014397
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014397.3, NP_055212.2
• RefSeq Size: 2596 bp
• RefSeq ORF: 942 bp
• Locus ID: 10783
• UniProt ID: Q9HC98
• Cytogenetics: 9q33.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 5 and 6 encode the same isoform (2).