NSDHL (NM_015922) Human Untagged Clone

CAT#: SC114562

NSDHL (untagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NSDHL
• Synonyms: H105E3; SDR31E1; XAP104
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114562 sequence for NM_015922 edited (data generated by NextGen Sequencing)
  ATGGAACCAGCAGTTAGCGAGCCAATGAGAGACCAAGTCGCACGGACTCATTTGACAGAG
  GACACTCCCAAAGTGAATGCTGACATAGAAAAGGTTAACCAGAATCAGGCCAAGAGATGC
  ACAGTGATCGGGGGCTCTGGATTCCTGGGGCAGCACATGGTGGAGCAGTTGCTGGCAAGA
  GGATATGCTGTCAATGTATTTGATATCCAGCAAGGGTTTGATAATCCCCAGGTGCGGTTC
  TTTCTGGGTGACCTCTGCAGCCGACAGGATCTGTACCCAGCTCTGAAAGGTGTAAACACA
  GTTTTCCACTGTGCGTCACCCCCACCATCCAGTAACAACAAGGAGCTCTTTTATAGAGTG
  AATTACATTGGCACCAAGAATGTCATTGAAACTTGCAAAGAGGCTGGGGTTCAGAAACTC
  ATTTTAACCAGCAGTGCCAGTGTCATCTTTGAGGGCGTCGATATCAAGAATGGAACTGAA
  GACCTTCCCTATGCCATGAAACCCATTGACTACTACACAGAGACTAAGATCTTACAGGAG
  AGGGCAGTTCTGGGCGCCAACGATCCTGAGAAGAATTTCTTAACCACAGCCATCCGCCCT
  CATGGCATTTTCGGCCCAAGGGACCCGCAGTTGGTACCCATCCTCATCGAGGCAGCCAGG
  AACGGCAAGATGAAGTTCGTGATTGGAAATGGGAAGAACTTGGTGGACTTCACCTTTGTG
  GAGAACGTGGTCCATGGACACATCCTGGCGGCAGAGCAGCTCTCCCGAGACTCGACACTG
  GGTGGGAAGGCATTTCACATCACCAATGATGAGCCCATCCCTTTCTGGACATTCCTGTCT
  CGCATCCTGACAGGCCTCAATTATGAGGCCCCCAAGTACCACATCCCCTACTGGGTGGCC
  TACTACCTGGCCCTCCTGCTATCCCTGCTGGTGATGGTGATCAGTCCTGTCATCCAGCTG
  CAGCCCACCTTCACACCCATGCGGGTCGCACTGGCTGGCACATTCCACTACTACAGCTGC
  GAGAGAGCCAAAAAGGCCATGGGCTACCAGCCACTAGTGACCATGGATGATGCTATGGAG
  AGGACCGTGCAGAGCTTTCGCCACCTGCGGAGGGTCAAGTGA
  
  Clone variation with respect to NM_015922.2
  132 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015922 unedited
  NNNGGTTCGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGT
  AAAGGGTGGCGGTCCGGGCCTGGAGTTCAGTGGGTGCAGCCTGCTTGCGAGCTGAGGCCA
  GACAGGGGGGCGCCTACGGACGGAAAAGAAAAGTTGATTACAAACGGGACCATATTTTGC
  TTCGAAATGGAACCAGCAGTTAGCGAGCCAATGAGAGACCAAGTCGCACGGACTCATTTG
  ACAGAGGACACTCCCAAAGTGAATGCTGACATAGAAAAGGTTAACCAGAATCAGGCCAAG
  AGATGCACAGTGATCGGGGGCTCTGGATTCCTGGGGCAGCACATGGTGGAGCAGTTGCTG
  GCAAGAGGATATGCTGTCAATGTATTTGATATCCAGCAAGGGTTTGATAATCCCCAGGTG
  CGGTTCTTTCTGGGTGACCTCTGCAGCCGACAGGATCTGTACCCAGCTCTGAAAGGTGTA
  AACACAGTTTTCCACTGTGCGTCACCCCCACCATCCAGTAACAACAAGGAGCTCTTTTAT
  AGAGTGAATTACATTGGCACCAAGAATGTCATTGAAACTTGCAAAGAGGCTGGGGTTCAG
  AAACTCATTTTAACCAGCAGTGCCAGTGTCATCTTTGAGGGCGTCGATATCAAGAATGGA
  ACTGAAGACCTTCCCTATGCCATGAAACCCATTGACTACTACACAGAGACTAAGATCTTA
  CAGGAGAGGGCAGTTCTGGGCGCCAACGATCCTGAGAAGAATTTCTTAACCACAGCCATC
  CGCCCTCATGGCATTTTCGGCCCAGGGACCCGCAGTTGGTACCCATCCTCATCGAGGCAG
  CCAGGAACGGGCAGATGAAGTTCGTGATTGGAAATGGNAAGAACTTGNNNTGACTCACCT
  TTGTGGAGAACGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015922 unedited
  NGACTCATGGNTAGAGATCATAGTAACTGCCTGCTTCAANAAATCCAAATCCAGTTCCAT
  GAAGGAAGAAAGTCTGTTTTTGCCGCCCTCATCGTCACGGAAAGAGTAGGGTGCGCTCTC
  TGCCTAGCAGAAGGAGTCACAGGCTCAGAGCAAACTCATTCAAAGGATGTTATTTCATCA
  ATCCACAGGGGAAGGAGTGACTGGCTGAGCAACGTGTCTAGAGAGCCCAGCCTCCAGTGT
  CCCTCACTTGACCCTCCGCAGGTGGCGAAAGCTCTGCACGGTCCTCTCCATAGCATCATC
  CATGGTCACTAGTGGCTGGTAGCCCATGGCCTTTTTGGCTCTCTCGCAGCTGTAGTAGTG
  GAATGTGCCAGCCAGTGCGACCCGCATGGGTGTGAAGGTGGGCTGCAGCTGGATGACAGG
  ACTGATCACCATCACCAGCAGGGATAGCAGGAGGGCCAGGTAGTAGGCCACCCAGTAGGG
  GATGTGGTACTTGGGGGCCTCATAATTGAGGCCTGTCAGGATGCGAGACAGGAATGTCCA
  GAAAGGGATGGGCTCATCATTGGTGATGTGAAATGCCTTCCCACCCAGTGTCGAGTCTCG
  GGAGAGCTGCTCTGCCGCCAGGATGTGTCCATGGACCACGTTCTCCACAAAGGTGAAGTC
  CACCAAGTTCTTCCCATTTCCAATCACGAACCTCATCTTGCCGTTTCTGGCTGCCTCGAT
  GAGGATGGGTACCAACTGCGGTTCCCTCGGGCCGAAAATGCCATGAGGGCGGATGGCTGT
  GGTTAAGAAATTCTCTCAGGATCGTTGGCGCCCAAAACTGCCCTTTCCTGTAAGACCTTA
  GTCTCTGTGTAGAACTCAATGGGTTCATGGCCTATGGAAGTCCTTAATTCCATTCTTGAT
  ATCAACCCCTCAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015922
• Insert Size: 1630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015922.1, NP_057006.1
• RefSeq Size: 1563 bp
• RefSeq ORF: 1122 bp
• Locus ID: 50814
• UniProt ID: Q15738
• Cytogenetics: Xq28
• Domains: 3Beta_HSD
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the more predominant transcript. Transcript variants 1 and 2 encode the same protein.