Androgen Receptor (AR) (NM_000044) Human Untagged Clone

CAT#: SC114220

AR (untagged)-Human androgen receptor (AR), OriGene unique variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Androgen Receptor
• Synonyms: AIS; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000044 edited
  AGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG TCAGAATT
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACG AGGAAAAACAAAAAAG
  CCGAAATAAAAGAAAAAGATAATAACTCAGTTCT TATTTGCACCTACTTCAGTGGACA
  CTGAATTTGGAAGGTGGAGGATTTTG TTTTTTTCTTTTAAGATCTGGGCATCTTTTGA
  ATCTACCCTTCAAGTATT AAGAGACAGACTGTGAGCCTAGCAGGGCAGATCTTGTCCA
  CCGTGTGTCT TTTTCTGCACGAGACTTTGAGGCTGTCAGAGCGCTTTTTGCGTGGTTG
  CT CCCGCAAGTTTCCTTCTCTGGAGCTTCCCGCAGGTGGGCAGCTAGCTGCA GCGA
  CTACCGCATCATCACAGCCTGTTGAACTCTTCTGAGCAAGAGAAGG GGAGGCGGGGTA
  AGGGAAGTAGGTGGAAGATTCAGCCAAGCTCAAGGATG GAAGTGCAGTTAGGGCTGGG
  AAGGGTCTACCCTCGGCCGCCGTCCAAGAC CTACCGAGGAGCTTTCCAGAATCTGTTC
  CAGAGCGTGCGCGAAGTGATCC AGAACCCGGGCCCCAGGCACCCAGAGGCCGCGAGCG
  CAGCACCTCCCGGC GCCAGTTTGCTGCTGCTGCAGCAGCAGCAGCAGCAGCAGCAGCA
  GCAGCA GCAGCAGCAGCAGCAGCAGCAGCAGCAAGAGACTAGCCCCAGGCAGCAGC
  AGCAGCAGCAGGGTGAGGATGGTTCTCCCCAAGCCCATCGTAGAGGCCCC ACAGGCTA
  CCTGGTCCTGGATGAGGAACAGCAACCTTCACAGCCGCAGTC GGCCCTGGAGTGCCAC
  CCCGAGAGAGGTTGCGTCCCAGAGCCTGGAGCCG CCGTGGCCGCCAGCAAGGGGCTGC
  CGCAGCAGCTGCCAGCACCTCCGGAC GAGGATGACTCAGCTGCCCCATCCACGTTGTC
  CCTGCTGGGCCCCACTTT CCCCGGCTTAAGCAGCTGCTCCGCTGACCTTAAAGACATC
  CTGAGCGAGG CCAGCACCATGCAACTCCTTCAGCAACAGCAGCAGGAAGCAGTATCCG
  AA GGCAGCAGCAGCGGGAGAGCGAGGGAGGCCTCGGGGGCTCCCACTTCCTC CAAG
  GACAATTACTTAGGGGGCACTTCGACCATTTCTGACAACGCCAAGG AGTTGTGTAAGG
  CAGTGTCGGTGTCCATGGGCCTGGGTGTGGAGGCGTTG GAGCATCTGAGTCCAGGGGA
  ACAGCTTCGGGGGGATTGCATGTACGCCCC ACTTTTGGGAGTTCCACCCGCTGTGCGT
  CCCACTCCTTGTGCCCCATTGG CCGAATGCAAAGGTTCTCTGCTAGACGACAGCGCAG
  GCAAGAGCACTGAA GATACTGCTGAGTATTCCCCTTTCAAGGGAGGTTACACCAAAGG
  GCTAGA AGGCGAGAGCCTAGGCTGCTCTGGCAGCGCTGCAGCAGGGAGCTCCGGGA
  CACTTGAACTGCCGTCTACCCTGTCTCTCTACAAGTCCGGAGCACTGGAC GAGGCAGC
  TGCGTACCAGAGTCGCGACTACTACAACTTTCCACTGGCTCT GGCCGGACCGCCGCCC
  CCTCCGCCGCCTCCCCATCCCCACGCTCGCATCA AGCTGGAGAACCCGCTGGACTACG
  GCAGCGCCTGGGCGGCTGCGGCGGCG CAGTGCCGCTATGGGGACCTGGCGAGCCTGCA
  TGGCGCGGGTGCAGCGGG ACCCGGTTCTGGGTCACCCTCAGCCGCCGCTTCCTCATCC
  TGGCACACTC TCTTCACAGCCGAAGAAGGCCAGTTGTATGGACCGTGTGGTGGTGGTG
  GG GGTGGTGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG CGGC
  GGCGAGGCGGGAGCTGTAGCCCCCTACGGCTACACTCGGCCCCCTC AGGGGCTGGCGG
  GCCAGGAAAGCGACTTCACCGCACCTGATGTGTGGTAC CCTGGCGGCATGGTGAGCAG
  AGTGCCCTATCCCAGTCCCACTTGTGTCAA AAGCGAAATGGGCCCCTGGATGGATAGC
  TACTCCGGACCTTACGGGGACA TGCGTTTGGAGACTGCCAGGGACCATGTTTTGCCCA
  TTGACTATTACTTT CCACCCCAGAAGACCTGCCTGATCTGTGGAGATGAAGCTTCTGG
  GTGTCA CTATGGAGCTGTCACATGTGGAAGCTGCAAGGTCTTCTTCAAAAGAGCCG
  CTGAAGGGAAACAGAAGTACCTGTGCGCCAGCAGAAATGATTGCACTATT GATAAATT
  CCGAAGGAAAAATTGTCCATCTTGTCGTCTTCGGAAATGTTA TGAAGCAGGGATGACT
  CTGGGAGCCCGGAAGCTGAAGAAACTTGGTAATC TGAAACTACAGGAGGAAGGAGAGG
  CTTCCAGCACCACCAGCCCCACTGAG GAGACAACCCAGAAGCTGACAGTGTCACACAT
  TGAAGGCTATGAATGTCA GCCCATCTTTCTGAATGTCCTGGAAGCCATTGAGCCAGGT
  GTAGTGTGTG CTGGACACGACAACAACCAGCCCGACTCCTTTGCAGCCTTGCTCTCTA
  GC CTCAATGAACTGGGAGAGAGACAGCTTGTACACGTGGTCAAGTGGGCCAA GGCC
  TTGCCTGGCTTCCGCAACTTACACGTGGACGACCAGATGGCTGTCA TTCAGTACTCCT
  GGATGGGGCTCATGGTGTTTGCCATGGGCTGGCGATCC TTCACCAATGTCAACTCCAG
  GATGCTCTACTTCGCCCCTGATCTGGTTTT CAATGAGTACCGCATGCACAAGTCCCGG
  ATGTACAGCCAGTGTGTCCGAA TGAGGCACCTCTCTCAAGAGTTTGGATGGCTCCAAA
  TCACCCCCCAGGAA TTCCTGTGCATGAAAGCACTGCTACTCTTCAGCATTATTCCAGT
  GGATGG GCTGAAAAATCAAAAATTCTTTGATGAACTTCGAATGAACTACATCAAGG
  AACTCGATCGTATCATTGCATGCAAAAGAAAAAATCCCACATCCTGCTCA AGACGCTT
  CTACCAGCTCACCAAGCTCCTGGACTCCGTGCAGCCTATTGC GAGAGAGCTGCATCAG
  TTCACTTTTGACCTGCTAATCAAGTCACACATGG TGAGCGTGGACTTTCCGGAAATGA
  TGGCAGAGATCATCTCTGTGCAAGTG CCCAAGATCCTTTCTGGGAAAGTCAAGCCCAT
  CTATTTCCACACCCAGTG AAGCATTGGAAACCCTATTTCCCCACCCCAGCTCATGCCC
  CCTTTCAGAT GTCTTCTGCCTGTTATAACTCTGCACTACTCCTCTGCAGTGCCTTGGG
  GA ATTTCCTCTATTGATGTACAGTCTGTCATGAACATGTTCCTGAATTCTAT TTGC
  TGGGCTTTTTTTTCTCTTTCTCTCCTTTCTTTTTCTTCTTCCCTCC CTATCTAACCCT
  CCCATGGCACCTTCAGACTTTGCTTCCCATTGTGGCTC CTATCTGTGTTTTGAATGGT
  GTTGTATGCCTTTAAATCTGTGATGATCCT CATATGGCCCAGTGTCAAGTTGTGCTTG
  TTTACAGCACTACTCTGTGCCA GCCACACAAACGTTTACTTATCTTATGCCACGGGAA
  GTTTAGAGAGCTAA GATTATCTGGGGAAATCAAAACAAAAACAAGCAAACAAAAAAAA
  AAAAAA AAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGA
  TCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000044 unedited
  CCCCTGTTCAGGAATATTTGTNAATACGAACTNCACTATGAGGGNCTGGCCGCGNAATTC
  GGCACGAGGGAAAAACAAAAAAGCCGAAATAAAAGAAAAAGAAATAACTCAGTTCTTATT
  TGCACCTACTTCAGTGGACACTGAATTTGGAAGGTGGAGGATTTTGTTTTTTTCTTTTAA
  GATCTGGGCATCTTTTGAATCTACCCTTCAAGTATTAAGAGACAGACTGTGAGCCTAGCA
  GGGCAGATCTTGTCCACCGTGTGTCTTTTTCTGCACGAGACTTTGAGGCTGTCAGAGCGC
  TTTTTGCGTGGTTGCTCCCGCAAGTTTCCTTCTCTGGAGCTTCCCGCAGGTGGGCAGCTA
  GCTGCAGCGACTACCGCATCATCACAGCCTGTTGAACTCTTCTGAGCAAGAGAAGGGGAG
  GCGGGGTAAGGGAAGTAGGTGGAAGATTCAGCCAAGCTCAAGGATGGAAGTGCAGTTAGG
  GCTGGGAAGGGTCTACCCTCGGCCGCCGTCCAAGACCTACCGAGGAGCTTTCCAGAATCT
  GTTCCAGAGCGTGCGCGAAGTGATCCAGAACCCGGGCCCCAGGCACCCAGAGGCCGCGAG
  CGCAGCACCTCCCGGCGCCAGTTTGCTGCTGCTGCAGCAGCAGCAGCAGCAGCAGCAGCA
  GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGAGACTAGCCCCAGGCAGCAGCAGCA
  GCAGCAGGGTGAGGATGGTTCTCCCCAAGCCCATCGTAGAGGCCCCACAGGCTACCTGGT
  CCTGGATGAGGAACAGNCACCTTCACAGCCGCAGTCGGCCCTGGAGTGCCACCCCGAGAG
  AGGTTGCGTCCCTAGAGCTGNAGCCGCCGTGGCCGCCAGCAGGNGCTGCCGCAGCAGCTG
  CCAGCACTTCTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000044 unedited
  GGGGGGGNGGGCCCCCNCCCCCATTTTAAANNNNNNTTTTGTGTAGNCCGCGGCCGCTTT
  CTANNATCGAGTTTTTTTTTTTTTTTTTTGTTTGCTTGTTTTTGTTTTGATTTCCCCAGA
  TAATCTTAGCTCTCTAAACTTCCCGTGGCATAAGATAAGTAAACGTTTGTGTGGCTGGCA
  CAGAGTAGTGCTGTAAACAAGCACAACTTGACACTGGGCCATATGAGGATCATCACAGAT
  TTAAAGGCATACAACACCATTCAAAACACAGATAGGAGCCACAATGGGAAGCAAAGTCTG
  AAGGTGCCATGGGAGGGTTAGATAGGGAGGGAAGAAGAAAAAGAAAGGAGAGAAAGAGAA
  AAAAAAGCCCAGCAAATAGAATTCAGGAACATGTTCATGACAGACTGTACATCAATAGAG
  GAAATTCCCCAAGGCACTGCAGAGGAGTAGTGCAGAGTTATAACAGGCAGAAGACATCTG
  AAAGGGGGCATGAGCTGGGGTGGGGAAATAGGGTTTCCAATGCTTCACTGGGTGTGGAAA
  TAGATGGGCTTGACTTTCCCAGAAAGGATCTTGGGCACTTGCACAGAGATGATCTCTGCC
  ATCATTTCCGGAAAGTCCACGCTCACCATGTGTGACTTGATTAGCAGGTCAAAAGTGAAC
  TGATGCAGCTCTCTCGCAATAGGCTGCACGGAGTCCAGGAGCTTGGTGAGCTGGTAGAAG
  CGTCTTGAGCANGATGTGGGATTTTTTCTTTTGCATGNCATGATACGATCGAGTTCCTTG
  ATGTAGNTCATTCGAAGTTCATCAAAGAAATTTTGATTTTTCAGCCCATCCACTGGNATA
  ATGCTGAAGAGTANCAGTGCTTTCATGCACAGGAAATTCTGGGGGGGTGATTTTGNAGCC
  ATCCAAACTCCTGAAAGAAGTGCCTCATTCGGACACACTGCCTGTACCTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000044
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000044.2, NP_000035.2
• RefSeq Size: 4314 bp
• RefSeq ORF: 2763 bp
• Locus ID: 367
• UniProt ID: P10275
• Cytogenetics: Xq12
• Domains: HOLI, Androgen_recep, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Oocyte meiosis, Pathways in cancer, Prostate cancer
• Gene Summary: The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
  Transcript Variant: This variant (1, also known as AR-FL in PMID:20823238) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.