CLIC5 (NM_016929) Human Untagged Clone

CAT#: SC114142

CLIC5 (untagged)-Human chloride intracellular channel 5 (CLIC5), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLIC5
• Synonyms: DFNB102; DFNB103; MST130; MSTP130
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114142 sequence for NM_016929 edited (data generated by NextGen Sequencing)
  ATGACAGACTCGGCGACAGCTAACGGGGACGACAGGGACCCCGAGATCGAGCTCTTTGTG
  AAGGCTGGAATCGATGGAGAAAGCATCGGCAACTGTCCTTTCTCTCAGCGCCTCTTCATG
  ATCCTCTGGCTGAAAGGAGTCGTGTTCAATGTCACCACTGTGGATCTGAAAAGAAAGCCA
  GCTGACCTGCACAACCTAGCCCCCGGCACGCACCCGCCCTTCCTGACCTTCAACGGGGAC
  GTGAAGACAGACGTCAATAAGATCGAGGAGTTCCTGGAGGAGACCTTGACCCCTGAAAAG
  TACCCCAAACTGGCTGCAAAACACCGGGAATCCAACACAGCGGGCATCGACATCTTTTCC
  AAGTTTTCTGCCTACATCAAAAATACCAAGCAGCAGAACAATGCTGCTCTTGAAAGAGGC
  CTAACCAAGGCTCTAAAGAAATTGGATGACTACCTGAACACCCCTCTACCAGAGGAGATT
  GACGCCAACACTTGTGGGGAAGACAAGGGGTCCCGGCGCAAGTTCCTGGATGGGGATGAG
  CTGACCCTGGCTGACTGCAATCTGTTGCCCAAGCTCCATGTGGTCAAGATTGTGGCCAAG
  AAATACCGCAACTATGATATCCCGGCTGAGATGACAGGCCTGTGGCGGTACCTCAAGAAC
  GCCTATGCCCGTGATGAGTTCACCAACACCTGTGCAGCTGACAGTGAGATCGAGTTGGCC
  TACGCTGATGTCGCCAAACGCCTCAGCCGATCCTGA
  
  Clone variation with respect to NM_016929.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016929 unedited
  GTATTTTGTCATACGACTCACTATAGGGCGGCCGGCGATTCGGCACGAGGGTCGCGGATC
  CTGTGACACCNTCCGGCAGCCCGGCACTTGTTGCTCCCACGACCTGTTGTCATTCCCTTA
  ACCCGGCTTTCCCCGTGGCCCCCCGCCTCCTCCCGGCTTCGCTCCTTTTCATGTGAGCAT
  CTGGGACACTGATCTCTCAGACCCCGCTGCTCGGGCTGGAGAATAGATGGTTTTGTGAAA
  AATTAAACACCGCCCTGAAGAGGAGCCCCGCTGGGCAGCGGCAGGAGCGCAGAGTGCTGG
  CCCAGGTGCTGCAGAGGTGGCGCCTCCCCGGCCCGGGACGGTAGCCCCGGGCGCCAACGG
  CATGACAGACTCGGCGACAGCTAACGGGGACGACAGGGACCCCGAGATCGAGCTCTTTGT
  GAAGGCTGGAATCGATGGAGAAAGCATCGGCAACTGTCCTTTCTCTCAGCGCCTCTTCAT
  GATCCTCTGGCTGAAAGGAGTCGTGTTCAATGTCACCACTGTGGATCTGAAAAGAAAGCC
  AGCTGACCTGCACAACCTAGCCCCCGGCACGCACCCGCCCTTCCTGACCTTCACGGGGGA
  CGTGAAGACAGACGTCAATAAGATCGGAGGAGTTCCCTGGAGGAGACCTTGACCCCCTGA
  AAGTACCCCAAACTGGCTGCAAAACACCGGGAATCCAACACAGCGGGCATCGACATCTTT
  TCCCAAGGTTTCTGCCTACATCAAAAATACCAAGCCGCAGACCAATGCTGGTATTCTGCC
  AGGGGGGTGGGGAGCCTGCCAGTGGAATGAAGGGGGACCGGATTTCTAAATGAGCTACTC
  CCCTCTGAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016929 unedited
  CGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTCACCTGGCAGTTGAGTCAGATTGTA
  GGAAAATTAACCCAGATGGGTCTACATTTTTCTTCAAGTTCAAACCACATGGTCTTCCTA
  GTCAGAAAGTCTCATGGACTTTCTTCCTAAGGTGTTCTATGATCAGACCACCTCCTAAAT
  GTGGCTTTTACCCATTACAGGCTACAGTTGAATCAGGCAGGAGCAGCTGCTGGAGAGCAC
  CCAGCCGACAGACCTGCATTCCAGAAGCAGCTTGGAGAAACTGGGAAGACATTTTTGACC
  CATTCTTGGGTTTTTACTGTAAGGTTAGCAGTTACATGATGCCACCAGTCCAAGACTTAT
  ATCATGAGAGAAGGCCTTTTTAAAAGTTTACCAGGGAACATCGAGGCTCATAAAGAACTC
  TTTTGTCTTCACCCATCTCTCCTCCTCCCACTCCCATCCACTTTCTCATAGCTCAAAAAC
  CACTCATTGGTAAGCACAGTCAAACCCAGGCCTGTAATCACCTCTGAAACAACTACTCCT
  AAGCAAGCAAACCCCATGGCTGGTAGACCCCGTGGGTATTTTGGCATCAACACTGCTCCC
  CCTCCCCTCCATCCCAGTCACTGCAGTTTAGAATTGCATGCCAATTTCCTTTTCTGGCAG
  GGCCTGGACAGCATGGAAGCCAGGGACAGCATGTGCCAGGTGCGCAGCAGCAGCAGCGCA
  GCATTCCTGGCACTGCAACAAACCTGAATTGTGTCTCAAGTGACTTTCCAGTCTGGCCCT
  TATTGCCCAATACCAGAGTGCCAAAGTAGTGAGACTGAAGCTGTGTNGCCAGACCCCAAG
  TCCTAGCCAGCTCGGACTCCTCATTCACTCTCTCTGCTCCCCTATGGGGAATTATACCCC
  TTCTAAAAAGATGATAAGCCGTTGTCCCTGCCCCTTGAGTATGACCGGAAGAAGGTNAAA
  AGATTTGTGTTTCATGTTCTAAGCTCTGAGAGGATTTGAATTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016929
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016929.1, NP_058625.1
• RefSeq Size: 2380 bp
• RefSeq ORF: 756 bp
• Locus ID: 53405
• UniProt ID: Q9NZA1
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Ion Channels: Other
• Gene Summary: This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b, also known as CLIC5A) is shorter and has a distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.