SMPD4 (NM_017751) Human Untagged Clone

CAT#: SC114022

SMPD4 (untagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMPD4
• Synonyms: NEDMABA; NEDMEBA; NET13; NSMASE-3; NSMASE3; SKNY
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017751, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACGACTTTCGGCGCCGTGGCGGAATGGCGGCTTCCATCTCTGAGGCGAGCGACGCTATGGATCCCAC
  AGTGGTTTGCTAAGAAGGCCATTTTCAACTCTCCACTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCA
  GCCCAGCTTTCTACTGGCTAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGAC
  TTGGTTAAAGTCATTGAGGACTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGCTGGTAGAAAGCA
  TTTTTGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCTTACAGGGGCGCGTGAATCCTGT
  GGAGTACAGCATCGTGATGGAATTTCTCGACCCTGGTGGCCCAATGATGAAGTTGGTTTATAAGCTTCAA
  GCTGAAGACTATAAGTTCGACTTTCCTGTCTCCTACTTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGT
  GCATCCTCCCTGACAGTCCTCTGTACCACAACAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAA
  CTTGGCCCTGAATCCGTTCGAGTATTACATATTCTTCTTTGCCTTGAGCCTCATCACTCAGAAGCCACTT
  CCTGTGTCCCTCCACGTCCGTACTTCAGACTGTGCCTATTTCATCCTGGTGGACAGGTACCTGTCATGGT
  TCCTGCCCACCGAAGGCAGTGTGCCCCCACCACTCTCCTCCAGCCCAGGGGGGACCAGCCCCTCACCACC
  TCCCAGGACACCAGCCATACCCTTTGCTTCCTATGGCCTCCACCACACTAGCCTCCTAAAGCGACACATC
  TCTCATCAGACGTCTGTGAATGCAGACCCCGCCTCCCACGAGATCTGGAGGTCAGAAACTCTGCTCCAGG
  TTTTTGTTGAAATGTGGCTTCATCACTATTCCTTGGAGATGTATCAAAAAATGCAGTCCCCTCATGCCAA
  GGAGTCGTTCACGCCTACTGAGGAGCATGTGTTGGTGGTGCGCCTGCTGCTGAAGCACCTGCACGCCTTT
  GCCAACAGCCTGAAGCCAGAGCAGGCCTCACCCTCCGCCCACTCCCACGCCACCAGCCCCCTGGAGGAGT
  TCAAACGGGCTGCTGTCCCGAGGTTCGTCCAGCAGAAACTCTACCTCTTCTTGCAGCATTGCTTTGGCCA
  CTGGCCCCTGGACGCATCGTTCAGAGCTGTCCTGGAGATGTGGCTGAGCTACCTGCAGCCGTGGCGGTAC
  GCGCCTGACAAGCAGGCTCCGGGCAGCGACTCCCAGCCCCGGTGTGTGTCGGAGAAATGGGCACCCTTTG
  TCCAGGAGAACCTGCTGATGTACACCAAGTTGTTTGTGGGCTTTCTGAACCGCGCGCTCCGCACAGACCT
  GGTCAGCCCCAAGCACGCGCTCATGGTGTTCCGAGTGGCCAAAGTCTTTGCCCAGCCCAACCTGGCTGAG
  ATGATTCAGAAAGGTGAGCAGCTATTCCTGGAGCCAGAGCTGGTCATCCCCCACCGCCAGCACCGACTCT
  TCACGGCCCCCACATTCACTGGGAGCTTCCTGTCACCCTGGCCACCAGCGGTCACTGATGCCTCCTTCAA
  GGTGAAGAGCCACGTCTACAGCCTGGAGGGCCAGGACTGCAAGTACACCCCGATGTTTGGGCCCGAGGCC
  CGCACCCTGGTCCTGCGCCTCGCTCAGCTCATCACACAGGCCAAACACACAGCCAAGTCCATCTCCGACC
  AGTGTGCGGAGAGCCCGGCTGGCCACTCCTTCCTCTCATGGCTGGGCTTTAGCTCCATGGACACCAATGG
  CTCCTACACAGCCAACGACCTGGACGAGATGGGGCAAGACAGTGTCCGGAAGACAGATGAATACCTGGAG
  AAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCGCAGCTCAGGCAGTTCACACTCGCCT
  TGGGCACCACCCAGGATGAGAATGGAAAAAAGCAACTCCCCGACTGCATCGTGGGTGAGGACGGACTCAT
  CCTTACGCCCCTGGGGCGGTACCAGATCATCAATGGGCTGCGAAGGTTTGAAATTGAGTACCAGGGGGAC
  CCGGAGCTGCAGCCCATCCGGAGCTATGAGATCGCCAGCTTGGTCCGCACACTCTTTAGGCTGTCGTCTG
  CCATCAACCACAGATTTGCAGGACAGATGGCGGCTCTGTGTTCCCGGGATGACTTCCTCGGCAGCTTCTG
  TCGCTACCACCTCACAGAACCTGGGCTGGCCAGCAGGCACCTGCTGAGCCCTGTGGGGCGGAGGCAGGTG
  GCCGGCCACACCCGCGGCCCCAGGCTCAGCCTGCGCTTCCTGGGCAGTTACCGGACGCTGGTCTCGCTGC
  TGCTGGCCTTCTTCGTGGCCTCTCTGTTCTGCGTCGGGCCCCTCCCATGCACGCTGCTGCTCACCCTGGG
  CTATGTCCTCTACGCCTCTGCCATGACACTGCTGACCGAGCGGGGGAAGCTGCACCAGCCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017751 unedited
  CCATTATGATACGACTCACTTAGGCGGCCGCGAATCGCACGAGGCGGAATGGCGGCTTCC
  ACCTCTGAAGCGGGCGACGCTATGGGTCCCACAGTGGTTTGCTAAGAAGGCCATTTTCAA
  CTCTCCATTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCAGCCCAGCTTTCTACTGGC
  CAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGACTTGATTAA
  AGCCATTGAGGATTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGCTGGCAGAGAG
  CATTTTCGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCTTACAGGGGCG
  TGTGAATCCTGTGGAGTACAGCATCGTGATGGAATTTCTCGACACTGGGTAGTGGCCCAA
  TGATGAAGTTGGTTTATAAGCTTCAAGCTGAAGACTATAAGTTCGACTTTCCTGTCTCCT
  ACCTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGTGCATCCTCCCTGACAGTCCTCTGT
  ACCACAGCAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAACCTGGCCCTGAATC
  CATTCGAGTATTACATACTCTTCTTTGCCCTGAGCCTCATCACTCAGAAGCCACTCCCTG
  TGTCCCTTCACGTCCGTACTTTAGACTGTGCCTATTTCATCCTGGCGGACAGGCACCTGT
  CATGGTTCCTGCCCACCGAAGCTAGTGTGCCCTGCCACTCTCCTCCAGCCAGGGGGGACC
  AGCCCCTCACCGCCTCCCAAAAATGTATATGCGGGCTGGGAGTGGCGGCTGACCCCTGTA
  ATTCCACCCTTGGGCGGGCCCAGCGGACGGACCCCCTGGGCCACGATTCCAAACCGCCTG
  GCCAAATGTGAAACCCCTTCCTCAATACACCAAGTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017751 unedited
  GTCTCATGTNACACGACGGNCACGGCAATTCTTAGAATACGAAGGTTTTTTCTTTTTTTT
  CTTTTTTTCTTTTTTTCTTTTTTTCTTTTACAAAACCAGGGAACAGTGTTTTATTCAGAA
  CCCATGGGGCGTTACAAGCTTATTTTTTGGCCACAGGCCCTAAAAGGCCTTTATTCTAAG
  TTTACATAAAAATACAAAGCCCAACTTTTACAATTTAAAAAACATTAAAGCAAACCCCCA
  AAACCCCACACCGAAAAACAAAGGTTGGGTTGGGAAATAACCACTGAAATGCTGTTCTCC
  CTCGAATGTCCCCTGGGGGGGGAATGAAGGGGAAAACCCCGGGCTGGTTTTTGTAAAAAA
  ATCTGAAGTTGTATATTTGGAATACTTAAGTACCACTGAACGGTGGCCATGTTTTTGACC
  ACTTGATGTTACTGAAGGGACAATGCTTCTGACCATTTTTGGCTGCAAAGCAAAAACTGG
  AAAAATCCGACCCCATCGACCCAGAAGTGTCCCCAACGCCTGAGGACCGGCCCCCATTTG
  TGACAAGGCACAGGGGTCCGCGATGTTGGCCACTGCTTTGGGGGATGGGCTCAATGGTTT
  GGGGCCTGCGGGGAACCACCTGGGCTCTGGGAGGGTTTTAGGCCTCCTGAAAATGGAATT
  CACGGCCCATTCTTGGAACAGCTGGGAAAACATGAAAAACGCCCCCCAGAGGGAAGAATC
  AGGAAAGCCACTCTTTGGTTTTCTCACACCTTTAAAAATTTTGATTCTTTCCTGGAGGGG
  AGACTTAACCCACCGGCCCTGGTCCCAACAAAGAAAGAGGAGGCGCCAAAACCCACTCTG
  GCTTACTTAAGGCCACCCAGTGGTGAGGGCCGGCCCAGGCAATTTTTGGGAGGTTGGTCC
  CAAACTTTGGTGAACAAAATACCACATAGGCCCCTGGAAAAAGGCCCCGGAAGATGGCTC
  TGGGCATGAATCTTTGGCAAAGGAAGGGAAAAGGCCGCCTTTAACTCTGGCTTGTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017751
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017751.1, NP_060221.1
• RefSeq Size: 3833 bp
• RefSeq ORF: 2397 bp
• Locus ID: 55627
• UniProt ID: Q9NXE4
• Cytogenetics: 2q21.1
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region, compared to variant 2, resulting in an isoform (1) that is shorter than isoform 2. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon. While this start codon is well-conserved, it should be noted that other than human and chimp, all other species have indels that follow this start codon. Other primates and rodents have frameshifting indels, and thus cannot use this start codon. However, some mammalian species, including cow, pig and dog, contain additional compensating indels that bring them back in frame; these species could therefore use the start codon represented in this CCDS. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 39 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.