TMEM100 (NM_018286) Human Untagged Clone

CAT#: SC113596

TMEM100 (untagged)-Human transmembrane protein 100 (TMEM100), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEM100
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113596 sequence for NM_018286 edited (data generated by NextGen Sequencing)
  ATGACTGAAGAGCCCATCAAGGAGATCCTGGGAGCCCCAAAGGCTCACATGGCAGCGACG
  ATGGAGAAGAGCCCCAAGAGTGAAGTTGTGATCACCACAGTCCCTCTGGTCAGTGAGATT
  CAGTTGATGGCTGCTACAGGGGGTACCGAGCTCTCCTGCTACCGCTGCATCATCCCCTTT
  GCTGTGGTTGTCTTCATCGCCGGCATCGTGGTCACCGCGGTGGCTTACAGCTTCAATTCC
  CATGGGTCTATTATCTCCATCTTTGGCCTGGTTGTTCTGTCATCTGGACTTTTTTTACTA
  GCCTCCAGTGCCTTGTGCTGGAAAGTGAGACAAAGGAGCAAGAAAGCCAAGAGACGGGAG
  AGTCAAACAGCTCTCGTGGCAAATCAGAGAAGCTTGTTTGCTTGA
  
  Clone variation with respect to NM_018286.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018286 unedited
  GCACGAGGAGGAAAGCCTTGCTGTTAGAGGCTCTTTCCCATAAGTTGGACGAAGAGGCTC
  ATGCGTTGCTGTTTCTTGTCTTCCAAGTCAAGTGGTTACTCTGGTAATGGATTGCCTCTC
  TCCGAGGTGAGTCTTTCACCCTGGTGAGACTGTCCAGATCTAGTCTGTAAACCCAGCTTA
  TAAGCACTGTTGTAAAAATGACTGAAGAGCCCATCAAGGAGATCCTGGGAGCCCCAAAGG
  CTCACATGGCAGCGACGATGGAGAAGAGCCCCAAGAGTGAAGTTGTGATCACCACAGTCC
  CTCTGGTCAGTGAGATTCAGTTGATGGCTGCTACAGGGGGTACCGAGCTCTCCTGCTACC
  GCTGCATCATCCCCTTTGCTGTGGTTGTCTTCATCGCCGGCATCGTGGTCACCGCGGTGG
  CTTACAGCTTCAATTCCCATGGGTCTATTATCTCCATCTTTGGCCTGGTTGTTCTGTCAT
  CTGGACTTTTTTTACTAGCCTCCAGTGCCTTGTGCTGGAAAGTGAGACAAAGGAGCAAGA
  AAGCCAAGAGACGGGAGAGTCAAACAGCTCTCGTGGCAAATCAGAGAAGCTTGTTTGCTT
  GAGACTGAATACGACCAAATGGGCCATTGGGCCTGGAAAACGTGCTCTGACTTTGTCACC
  CAATTCACCCAGAACCATGGTGGGAGAGAACAGACTTGGCGTTGGAGCAGACTGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018286 unedited
  TACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTCCCAAAATGTAA
  TATACATTTAATAGCACATTATAAAGTTCCTGACCAAAGACGTTGATTTCCTAATTATAA
  TAGCACAGAAATCCTTTAGAATTTAGTAAACGTAATTAAGACTATTCAGAAGTAATGAAA
  AACCAATATGATAAAAACAAAAATCCTCCAGTAAAGAAGGAACCTGTCCATTTGAGAGAA
  ATACAATTGAGAACTTGCAAATGAGACAAGGGAAGATGGCAATTTGGAACTGCAATAGAA
  ATAACTATAGCAGAAACAACCATTTAAGAAGTTTTAGCAGCAATAAGTATTTATTATTCT
  GAATGAAATGTACAGTTGACTTTTATATAAAAATCATCAAAAGTGCTATATTGGATTATT
  TTACTATTAATTTAACCCCCAACAGCATCTATTAGCTATAACTTTAATGGGTTCTTCTTT
  ACTTCTGATACATCATTACAACTGGCTAACACTGTCCAATGTTAAAATATAAAAAATATT
  CCTTTTCAAGATAAAGTACCACCAGTGATGAGTTATTTAAAGTATAAAATGAAAACAGAC
  GCCCGGGAATCATGGGTTGAAAAAAATATCACAGAATTTCATAGACCTATGCGGAACCCA
  AGGTAATTTAGATCCTTGGAAAACAATTTTTAGGATACATCTTAAGAACTGGGGGTATTT
  TTTTTGGGGGGAAATTGTTAGTGGGGCCCGGCCTTACTTGGATTTTCCCACTCTTTTTTG
  GGCTCTTTCAGGGGGGAACCAAACCGGGTAGAAGGGGGGCCCTCCTTGACCCCACAAGGT
  TTCTGGAAAAAAAAGCGGGTTTTCGGTCTAAAATGCCCTCTGGGTAAGTGGGTGTAAAGA
  AACTGGCTTTCCTACAAGGAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018286
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018286.1, NP_060756.1
• RefSeq Size: 1670 bp
• RefSeq ORF: 405 bp
• Locus ID: 55273
• UniProt ID: Q9NV29
• Cytogenetics: 17q22
• Protein Families: Transmembrane
• Gene Summary: Plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. Involved in the regulation of nociception, acting as a modulator of the interaction between TRPA1 and TRPV1, two molecular sensors and mediators of pain signals in dorsal root ganglia (DRG) neurons. Mechanistically, it weakens their interaction, thereby releasing the inhibition of TRPA1 by TRPV1 and increasing the single-channel open probability of the TRPA1-TRPV1 complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.