CHST11 (NM_018413) Human Untagged Clone

CAT#: SC113500

CHST11 (untagged)-Human carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHST11
• Synonyms: C4ST; C4ST-1; C4ST1; HSA269537; OCBMD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113500 sequence for NM_018413 edited (data generated by NextGen Sequencing)
  ATGAAGCCAGCGCTGCTGGAAGTGATGAGGATGAACAGAATCTGCCGGATGGTGCTGGCC
  ACTTGCTTGGGATCCTTTATCCTGGTCATCTTCTATTTCCAAAGTATGTTGCACCCAGTC
  ATGCGGAGGAATCCCTTTGGTGTGGACATCTGCTGCCGGAAGGGGTCCCGAAGCCCCCTG
  CAGGAACTCTACAACCCAATCCAGCTGGAGCTCTCAAACACTGCTGTCCTGCACCAGATG
  CGGCGGGACCAGGTGACAGACACGTGCCGAGCCAACAGCGCCACAAGCCGTAAGCGGAGG
  GTGCTGACCCCCAACGACCTGAAGCACTTGGTGGTGGATGAGGACCACGAGCTCATCTAC
  TGCTACGTGCCCAAGGTGGCCTGCACCAACTGGAAGCGGCTCATGATGGTCCTGACCGGG
  CGGGGGAAGTACAGCGACCCCATGGAGATCCCGGCCAACGAGGCACACGTCTCCGCCAAC
  CTGAAGACCCTGAACCAGTACAGCATCCCAGAAATCAACCACCGCTTGAAAAGCTACATG
  AAGTTCCTGTTTGTCCGGGAGCCCTTCGAGAGGCTAGTGTCCGCCTACCGCAACAAGTTC
  ACCCAGAAGTACAACATCTCCTTCCACAAGCGGTACGGCACCAAGATCATCAAACGCCAG
  CGGAAGAACGCCACCCAGGAGGCCCTGCGCAAAGGGGACGATGTCAAATTCGAGGAGTTT
  GTGGCCTATCTCATCGACCCACACACCCAGCGGGAGGAGCCTTTCAACGAACACTGGCAA
  ACCGTCTACTCACTCTGCCATCCCTGCCACATCCACTATGACCTCGTGGGCAAGTACGAG
  ACACTGGAAGAGGATTCTAATTACGTCCTGCAGCTGGCAGGAGTGGGCAGCTACCTGAAG
  TTCCCCACCTATGCAAAGTCTACGAGAACTACTGATGAAATGACCACAGAATTCTTCCAG
  AACATCAGCTCAGAGCACCAAACGCAGCTGTACGAAGTCTACAAACTCGATTTTTTAATG
  TTCAATTACTCAGTGCCAAGCTACCTGAAATTGGAATAA
  
  Clone variation with respect to NM_018413.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018413 unedited
  GCATTTTGTATACGACTACTATAGGCGGCNCGCGATTCGGCACGAGGCTCCCCTCGCCGG
  GGGCCGCGAGTTGCATTTGGTAAAACCCAGCCCCGGAATATATAGATCGTTGGAGCGCAA
  TGAAGTAGCCTTTGGAGAGAAGGGAGAGGGCCCGTCGGACAGCCACAGCGGCCAGCGCAG
  CGGCAGCGGCGGCGGCACCACCATCACCGCTCGCACCCCAGCCGCCCGGCCCGCGACCAG
  GCAGCGGCGGCCGCCGGCGGGATCGGAGGAGGCGGCGGAGCGGCGAGGAGGAGGAGCAGG
  AGCGCGCAGCCAGCGGGTCCACGCATCTCAGCACTTCCAGACCAACTCCGGCACCTTCCA
  CACCCCTGCCCGGGCTGGGGGCTCCGAGAGCGGCCGCGAAGCGACTCCGATCCTCCCTCT
  GAGCCTTGCTCAGCTCTGCCCCGCGCCTCCCGGGCTCCGGTCCGCGCGGCGGGGTCCCTG
  CTCCTGCGCCCCGGGCGCGCTTCCCGGACACCCCGGTCCCCGCAGCCAGGACAAAGCCAT
  GAAGCCAGCGCTGCTGGAAGTGATGAGGATGAACAGAATCTGCCGGATGGTGCTGGCCAC
  TTGCTTGGGATCCTTTATCCTGGTCATCTTCTATTTCCAAAGTATGTTGCACCCAGTCAT
  GCGGAGGAATCCCTTTGGTGTGGACATCTGCTGCCGGAAGGGGTCCCGAAGCCCCCTGCA
  GGAACTCTNACACCCAATCCAGCTGGAGCTCTCAAACACTGCTGTCCTGCACAGATGCNG
  CGGNACCAGGTGACAGACACGTGCCGAGCCACAGCGCCACAGCCGTAGCGGAGGTGCTGA
  CCCCACGACTGAGCACTTGTTGGTGATGAGACACGAGCTATTACTGTTCTGCCCAGGTGC
  CTGCACACTGGAGCGCTCTGATGTCCTGACGNGCGGGGAGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018413 unedited
  AGCTTTGGACCGCGGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTAAAAAAACTATAAATAATATTAATTAAAATTTAGGGAAGGC
  CCAATTCATGGAATTCACCAAAACACAAAAAACCTTTGAAACAGGGTTGCAGTATTCACT
  TTTACAGACAAGGACGACCCATCCTATTTCCCCTGCAAAAACAGGTGTCCTTCCCTACAA
  AGGATTTTAAAAAATTCTCACTATGCGGCTCGCAGAATCATCCCCAAAAAAATATTAATT
  TACAAAATAACCCAATATCCATATAATTTTTTTGACAAATAAAAATTTTAAATTAAAAAG
  CATGATTTTTTCCCTTTCCCCACCCCTTTTATTCCAATTTCAGGTAGCTGGGCCCTGAGT
  AATTGAACATTAAAAAATCGAGTTTGAAAACTTCGAACAGCTGCGTTGGGGGCTCTGAGC
  TGATGTTCTGGAAAAATTCTGGGGCCATTTCATCAGAAGTTCTCGAAAACTTTGCATAGG
  GGGGGAACTTCAGGTAGCTGCCCACTCCTGCCAGCTGCAGGACGTAATTAAAATCCTCTT
  CCAGGGTCTCGTACTTGCCCACGAGGTCATAGGGGATGTGGCAGGGATGGCAAAGTGAGT
  AAACGGTTTGCCAGTGTTCGTTGAAAGGCTCCTCCCGCGTGGGTGTGTGGGTCGATGAAA
  TAGGCCACAAACTCCTCGATTTGACATCGCCCCCTGTGCGCAGGGCCTCCTGGTNGGGGT
  CCTCCGCTGGCGTTTGAGATCTGGGCGCGAACCGCTTGTGAAAGAAATGTGAACTTTTGG
  AGAACTGGTGGGTAAGGGGACATACCCTTTCAAGGGCTCCGGAAACAAGAACTTAAGGAG
  CTTTTAAGGGGGGTTATTCTGGGAAGCGACTGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018413
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018413.2, NP_060883.1
• RefSeq Size: 1687 bp
• RefSeq ORF: 1059 bp
• Locus ID: 50515
• UniProt ID: Q9NPF2
• Cytogenetics: 12q23.3
• Domains: Sulfotransfer2
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Sulfur metabolism
• Gene Summary: The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving this gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.