Macro H2A.2 (H2AFY2) (NM_018649) Human Untagged Clone

CAT#: SC113407

H2AFY2 (untagged)-Human H2A histone family, member Y2 (H2AFY2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Macro H2A.2
• Synonyms: H2AFY2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113407 sequence for NM_018649 edited (data generated by NextGen Sequencing)
  ATGTCGGGCCGGAGTGGGAAGAAGAAAATGTCCAAGCTGTCCCGTTCAGCTAGGGCAGGT
  GTCATCTTTCCAGTGGGGAGGCTGATGCGTTATCTGAAGAAAGGGACGTTCAAGTACCGG
  ATCAGCGTGGGCGCCCCTGTCTACATGGCGGCAGTCATTGAGTACCTGGCAGCGGAAATT
  CTAGAATTGGCCGGCAATGCCGCGAGGGACAACAAGAAGGCCCGGATAGCCCCGAGACAC
  ATCTTGCTGGCAGTTGCCAATGACGAGGAGCTCAACCAGCTGCTAAAAGGAGTGACCATC
  GCCAGTGGAGGCGTCCTGCCCAGAATTCACCCCGAACTGCTGGCCAAAAAGCGAGGGACC
  AAAGGCAAGTCGGAAACGATCCTCTCCCCACCCCCAGAGAAAAGAGGCAGGAAGGCCACG
  TCAGGCAAGAAGGGGGGGAAGAAATCCAAGGCTGCCAAACCACGGACGTCCAAAAAGTCC
  AAACCAAAGGACAGCGATAAAGAAGGAACTTCAAATTCCACCTCTGAAGATGGGCCAGGG
  GATGGATTCACCATTCTGTCTTCTAAGAGCCTTGTTCTGGGACAGAAGCTGTCCTTAACC
  CAGAGTGACATCAGCCATATTGGCTCCATGAGAGTGGAGGGCATTGTCCACCCAACCACA
  GCCGAAATTGACCTCAAAGAAGATATAGGTAAAGCCTTGGAAAAGGCTGGGGGAAAAGAG
  TTCTTGGAAACGGTAAAGGAGCTTCGCAAATCCCAAGGCCCTTTGGAAGTCGCCGAAGCC
  GCCGTCAGCCAATCCAGTGGACTCGCAGCCAAATTTGTCATCCACTGTCACATCCCTCAG
  TGGGGCTCCGACAAATGTGAAGAACAGCTTGAAGAGACCATCAAAAACTGCCTGTCAGCG
  GCGGAGGACAAGAAGCTAAAGTCCGTCGCGTTCCCGCCTTTCCCCAGCGGCAGAAACTGC
  TTTCCCAAACAGACTGCGGCCCAGGTGACCCTCAAAGCCATCTCAGCCCACTTTGATGAC
  TCGAGCGCGTCCTCGCTGAAGAACGTGTACTTCCTGCTCTTCGACAGCGAGAGCATCGGC
  ATCTACGTGCAGGAGATGGCCAAGCTCGACGCCAAGTAG
  
  Clone variation with respect to NM_018649.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018649 unedited
  CATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCTTGCCGCCA
  TTGACACGCACAGATAGAACCCAAAGAAAGGCAAAGAGTCCTGCCCGGCACCGGCGCCGC
  GTGGGCCAAACCTGCGCCCGTGGAGGGGCGCGCAGAGGGCACCGGGCGCCGGGAGCAGGC
  GGCGCAGCACCAGCATTGTGTTAGTGCCGGGAGGCCACTGTGTCAGCAAGCTGAGAGGGA
  AACTGAAGCAAGATGTCGGGCCGGAGTGGGAAGAAGAAAATGTCCAAGCTGTCCCGTTCA
  GCTAGGGCAGGTGTCATCTTTCCAGTGGGGAGGCTGATGCGTTATCTGAAGAAAGGGACG
  TTCAAGTACCGGATCAGCGTGGGCGCCCCTGTCTACATGGCGGCAGTCATTGAGTACCTG
  GCAGCGGAAATTCTAGAATTGGCCGGCAATGCCGCGAGGGACAACAAGAAGGCCCGGATA
  GCCCCGAGACACATCTTGCTGGCAGTTGCCAATGACGAGGAGCTCAACCAGCTGCTAAAA
  GGAGTGACCATCGCCAGTGGAGGCGTCCTGCCCAGAATTCACCCCGAACTGCTGGCCAAA
  AAGCGAGGGACCAAAGGCAAGTCGGAAACGATCCTCTCCCCACCCCCAGAGAAAAGAGGC
  AGGAAGGCCACGTCAGGCAAGAAGGGGGGGAAGAAATCCAAGGCTGCCAAACCACGGACG
  TCCAAAAAGTCCAAACCAAAGGACAGCGATAAAGAAGGAACTTCAAATTCCACCTCTGAA
  GATGGGCCAGGGGATGGNATCACCATTCTGTCTTCTAAGAGCTTGTTCTGGGACAGAAGC
  TGTCCTTACCCAGAGTGACATCAGCCATATGGNCTNCATGAGAGTGGNAGGGCATTGTNC
  ACCCAACCACAGCCCGAAATGACCTCCAAGAAGATATAAGTAAGCCTTGGAAAGGCTGNG
  GGAAAAGAGTCCTGGAACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018649 unedited
  GCAATCTAGAGTCGAGTCCCNCCCCTTTCCTTTCTTCTCTTTTTTTTTTTTTCTTTTCTT
  CCCCTTTTTTTTTTTTTCCTTAAAAAAACCACGGGATCTTTTATTCTTTTCCTTTCATTT
  TCTTTTCCTAAAAAAACAAAATGAAAAAAAAAAAACGCCTGAATCAAAACCTTTTTAGGA
  GTATTTACAGATTTTTTAGGGATGGGGGCGGGGGGCACTAAAACAAAAAAAAAAACCCCC
  AGGGAAATGTTTTTCCCATTTTTTTTTTTCCCCCCGGGAACACCCCCACCAACAAACCCC
  AGGCCCTTTTTTGCCCTTTTCCCTTGGAAAAAGGGGGAACAAAAAATTTAAAAATTTTGA
  AAGGATTTTTTTCTTGGGGAAAGGGGGGAAAAAGCAACTGCTATAAATCTAAACCGAGGT
  CTGTTTTTTTAAAAAAGTTCTAAACCACCGGGGGGAAACCCCCTGGAAAAAAACCCCTTG
  GGGAAACCTGTTTTTTTATAAATGGAGGTTTTTTTGAAGAAAAGTGGAAAGGGAAAAGGT
  CTCCTTGCCCCGGAAGGAACTGCCGGGACACCCTTCACTCCCTGGCAATAACCCCTTCTT
  TTTTTTTTTAAAAACAAAACCCCCCTTTTTGGAACTCCGGGCCCTCTCCCAACCCCTTGC
  TGAAAAAGGGGGGGGGCTTCCTTCGGCCGAAAACCTTGCCCTCTCCCTCGCCGAAAATGT
  CCAAGCTTTCCCCTTGTATAAAAAGAAAGAATCACACGTTTCTTTAGCAGGGACCCCGCT
  CAAATAATTAAAAGGGGCTTAAAATGGCTTTTAGGGTCACCCTGGCCCATATTTTGTTTG
  AGAAAACCATTTCTTCTCTTGTGGAAAGGCGAAACCGCACAGATTTTATTTTTTGCCCTC
  CCCGTACAAGAGATTTTTAGAGGTTCTCTTAACGTTTTCTACAATTGGCGGACCCCCCTT
  GGGGAGGGAACAGGTGAACACATTGCTTCAAACACCGTTTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018649
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018649.1, NP_061119.1
• RefSeq Size: 1932 bp
• RefSeq ORF: 1119 bp
• Locus ID: 55506
• UniProt ID: Q9P0M6
• Cytogenetics: 10q22.1
• Domains: H2A, A1pp, histone
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Oct 2015]