DCUN1D1 (NM_020640) Human Untagged Clone

CAT#: SC113101

DCUN1D1 (untagged)-Human DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) (DCUN1D1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCUN1D1
• Synonyms: DCNL1; DCUN1L1; RP42; SCCRO; SCRO; Tes3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113101 sequence for NM_020640 edited (data generated by NextGen Sequencing)
  ATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTTTATGATCTTCACACAA
  TCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTCAAAATGACTGGAAGTTAGATGTTGCA
  ACAGATAATTTTTTCCAAAATCCTGAACTTTATATACGAGAGAGTGTAAAAGGATCATTG
  GACAGGAAGAAGTTAGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAA
  ATTGGAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCCAGCATT
  AGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTGCGAGTTCTCCAAACAG
  GAGTTCATGGATGGCATGACAGAATTAGGATGTGACAGCATAGAAAAACTAAAGGCCCAG
  ATACCCAAGATGGAACAAGAATTGAAAGAACCAGGACGATTTAAGGATTTTTACCAGTTT
  ACTTTTAATTTTGCAAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCC
  TACTGGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAATTTTTG
  TTGGAACATCATAAACGATCAATACCAAAAGACACTTGGAATCTTCTTTTAGACTTCAGT
  ACGATGATTGCAGATGACATGTCTAATTATGATGAAGAAGGAGCATGGCCTGTTCTTATT
  GATGACTTTGTGGAATTTGCACGCCCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAG
  
  Clone variation with respect to NM_020640.2
  318 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020640 unedited
  NTTTCGGGATTTGTATACGATTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGAGCCG
  GAGGAGGAGGGGAGAGGCCTGNGAGACACCAACATGAACAAGTTGAAATCATCGCAGAAG
  GATAAAGTTCGTCAGTTTATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGT
  CTTTCTCAAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCTGAA
  CTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTTAGAACAGCTGTAC
  AATAGATACAAAGACCCTCAAGATGAGAATAAAATTGGAATAGATGGCATACAGCAGTTC
  TGTGATGACCTGGCACTCGATCCAGCCAGCATTAGTGTGTTGATTATTGCATGGAAGTTC
  AGAGCAGCAACACAGTGCGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTA
  GGATGTGACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTGAAA
  GAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGCAAAGAATCCAGGA
  CAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACTGGAACTTAGTGCTTAATGGAAGA
  TTTANATTCTTAGACTTATGGAATAAATTTTTGTTGGAACATCATAAACGATCAATACCA
  AAAGACACTTGGAATCTTCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAAT
  TATGATGAAGAAGGAGCATGGCCTGNTCTTATTGATGAACCTTGTGGAATTGCACGCCCT
  CAATTGCTGGGACAAAAGTACACAGTGTAGCACTAAAGGACCCTCTAGAATGTCCTAGTC
  TGTACATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020640 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGTCATGGCAATTTCACTGCAG
  CTTTTATTGCCTCTGTTGGTAATATGACAGGGACTGTAAGAAACACAATTGAACTTACAG
  AGATATGCAGATGATATTCACTAAAGATAATCTTCATCTCAAGTATTTTTTATATGTGGG
  CACTTCTAATTTTATACATTTTTCTATTTAAAAAGCATTACATTTTAAAATTTGTACCTG
  TTTTGACATTTGGCAAAAAGGAAACTGTACCTATGGTTCAGTTTAAAACAAAGAATCAGC
  ATGGGGAGTTTACCGGAATGAGGACTGAAACACTTAGGAGTTAAAGCAATTCAAGGGGCG
  TGTGTGTGTCTCTCCCTTTCTCTGTCAATCTCTCTCACTCTCTCTGGCTCTCTCACAGAC
  ACAGACTCAAACACACACAGCCATATGTGTAATCATTTTTAGTACAAAATATTCCCTGTA
  GCCAGACATTTATCTTCAGAATAATCACACCACATTAAATCAGTCTTCTCTTTTCAGCCC
  TAGTAAAAGTTTTGTTTAATTCTAAGATGCCATCATAGTGAACAACCTTNACTNNTATGC
  TACATANNATATATTCAAAGCTTTATGTATTTTTTGTTTATAATTACACAGTAATTATAA
  ATGTTTAGTACCAACCTGATGACAAACACTATTTTTGTGGAAAAGGTTTTGCATTTAAAA
  AGAAAAATATGTAAAATCCAAGGCACTAGGCCACCGACCAATTTGACTTTTAAAGGAAAC
  ACTTTATTTTGGAAAAAAAAAAAAAAAAAAAAGCTATGGGGATCTCATTTCAAACCCAGA
  TTTGTGAATTAATCATCTTGACCCCATACCTTACCTCAAATCTTCAAGACCGGAACAAAA
  CGGGCTTTTTTTTGATTTTGGATTATTTTCTTTTTAAATTCTCAATATTGAAGTTCTTTT
  ATATTCCAAAAAACGGGATACTGGAGCGCAGATTTTCTTTCCTTTTCTTTATACCATTCC
  GGCCCGAATGATTTTCAATTTCTTTGGAAACGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020640
• Insert Size: 3520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020640.2, NP_065691.2
• RefSeq Size: 3192 bp
• RefSeq ORF: 780 bp
• Locus ID: 54165
• UniProt ID: Q96GG9
• Cytogenetics: 3q26.33
• Domains: DUF298
• Protein Families: Druggable Genome
• Gene Summary: Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed:25349211, PubMed:28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.