VAMP4 (NM_003762) Human Untagged Clone

CAT#: SC111716

VAMP4 (untagged)-Human vesicle-associated membrane protein 4 (VAMP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAMP4
• Synonyms: VAMP-4; VAMP24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003762 edited
  GAATTCGGCACGAGGCGGCTTCCTACTCCTTCCCCCGCCCGCCCAGCACCGCCAGCCCGG
  GAGAGCTGCCGGGAGTTCCCCGGGAGCCTCAGGTGGTGACTATCCTGTTGAGAAGCAAAA
  GATACTTTGCAAGTAAAAAATATGCCTCCCAAGTTTAAGCGCCACCTCAATGATGATGAT
  GTCACAGGTTCTGTGAAAAGTGAAAGGAGAAATCTTTTGGAAGATGATTCAGATGAAGAA
  GAGGACTTTTTTCTAAGGGGACCATCTGGACCAAGATTTGGACCTAGAAATGATAAAATT
  AAGCATGTTCAGAATCAAGTGGATGAAGTTATTGATGTCATGCAAGAAAATATTACAAAG
  GTAATTGAGAGAGGGGAGAGACTAGATGAACTACAGGACAAATCAGAAAGCTTATCGGAT
  AATGCAACAGCTTTTAGCAACAGATCCAAACAACTTCGAAGGCAAATGTGGTGGCGTGGA
  TGCAAAATAAAAGCCATCATGGCTTTGGTTGCTGCTATCCTTTTGCTAGTGATTATCATT
  CTTATAGTCATGAAATACCGTACTTGATTTGATGACAGAGATCTTCATTAAACAAGATCT
  GGGACAGTAATAAAAGATTGCTGCATAATTTAAATGAAACCTATGTGTATATAACTTTCA
  AAACTTCTTTTTCAAGAAACTAAGAGGCAAGTATCACTTCAAATTGGAACGTTGAGAATG
  TCCAATTATCTTCTCCCTTTCTAACAAAATGTTCTTTTAATAATTATGTTTAAGGCAAAG
  AGAACTAGCCTCTATTTTTCCATATTTCAAGGATCTAATTTGAAACTAGATACTTGCCAG
  TTCATTATTTGTGTATATAAACACTGATTATAATATTTCATATTAATATTTTAATGGCAT
  AAGGACTTGCATTATTGCATTAGGGAGCGGGTCATGCTGGGGGTCAGGAAGCCTGTGTAG
  AGTACCAAATTATATGCTGAAAGAGATGCATAACCATTCTGTCAATATTTGCAGAAATAT
  AGTTCCTTTCATATTACAGTTGTTTTGCAAGCAATTTCCACATTTATAGGTGTAACAAAA
  GCTAAATGTTGTAAATGTTGTTGCCCTCAGGTATAACTAAAAACATTCCAGTAAATATAT
  TTTTGACTGTGTAAGAGAATGTGTAGTAATTTTTTTGGCATTGGATTATGGAAATGGAAA
  TTTTTAAACAGTGTGAAAAAACATGGTATGGCATCATAAAACTGGAGATAATAAAGTTAT
  TGAAGAGTGTCATTGGAGTATTTGAAAATGACCAATATATAGCTGCCAGTCATGGTCCAG
  TCTGTTTTCTGTTAAACTCCAATAAAGAGAGACCAGTCAGTCCTAATGTGAGTAAATGAA
  ATTTACAGGTAACTTACTTACTCCTTAATTTTATTTTAGTTAATAGCCTCTAACTTCCAA
  CTATCTCATTTACCAAAAGTACAAAAAGTAGCAAAACCATCTATTAATACTTTGAATTTT
  ATATCTTTGCATTTCCTGTTCATCCTTGAGGAGATGTATGTTGCACAATCAAATTTTGCA
  TCAGCCAACAGTAATGAGTAGTAGAAAGTCAGGAACCTAGGTGTATATATATATATATAT
  ATATATATATATATATATATATATACACACAAACACACACACACACACACACACACACGC
  ACACATATGATAATCAGTCAGTCGGTAACCAAATGCTAGAGAGTGACCATTAACCTAAGC
  CTGTTTTTXXXXXXXXXXXXXXXXXXXXXTTTTAGAATTTCAGTAGCTAATAATTCAGGA
  ATGGAAATATTTTAACTGAATGGACAGAATCAGTCCTAACATTTGTATGTATGTCCTTTA
  TTTGAATTGGAACTAGGCTCATAAGTGGTTAATTTTCCATTAGTTGATGTAGAAATCTGG
  TTTCTCCATAATATCTACAAGTGAAGATGAAGATTACTTCTCAAAAAGTATCCATATTTC
  CAGTGTTCCTTTTAATAAAATATGCCACTTTTAAATAAATAATTACAAAATGTAAAATTA
  TAAAATTTTTAAATTTAAATAATAAGTAACAGTAAAATTTTTTGACAATATATATGTCAC
  TGTCTTTGTAATTTAAACATATTGCTGCCTGGGATATGGGTAACAGAATTATCACTCATG
  GAACTTTGTAGTAGTGATGGCTTGGTTTGTAATTCATCATTTCATCTTTCTTGGTCTTGA
  TTAATCCTGAAATCCTTAAAATGGCTGGCATGGTTATGACTTTATTGTGGTAACCTTGGA
  GATATAAGCCCTCTTACGATCAAAAACAGAAGAAAAAAATATATTGTTCAAACTGAAAAA
  AAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003762 unedited
  NAACGCCTCATTTGGATACGACTTCTATAGGCGGCCGACGAATTCGCACGAGGCGGCTTC
  CTACTCCTTCCCCCGCCCGCCCATCACCGCCAGCCCGGGAGAGCTGCCGGGAGTTCCCCG
  GGAGCCTCAGGTGGTGACTATCCTGTTGAGAAGCAAAAGATACTTTGCAAGTAAAAAATA
  TGCCTCCCAAGTTTAAGCGCCACCTCAATGATGATGATGTCACAGGTTCTGTGAAAAGTG
  AAAGGAGAAATCTTTTGGAAGATGATTCAGATGAAGAAGAGGACTTTTTTCTAAGGGGAC
  CATCTGGACCAAGATTTGGACCTAGAAATGATAAAATTAAGCATGTTCAGAATCAAGTGG
  ATGAAGTTATTGATGTCATGCAAGAAAATATTACAAAGGTAATTGAGAGAGGGGAGAGAC
  TAGATGAACTACAGGACAAATCAGAAAGCTTATCGGATAATGCAACAGCTTTTAGCAACA
  GATCCAAACAACTTCGAAGGCAAATGTGGTGGCGTGGATGCAAAATAAAAGCCATCATGG
  CTTTGGTTGCTGCTATCCTTTTGCTAGTGATTATCATTCTTATAGTCATGAAATACCGTA
  CTTGATTTGATGACAGAGATCTTCATTAAACAAGATCTGGGACAGTAATAAAAGATTGCT
  GCATAATTTAAATGAAACCTATGTGTATATAACTTTCAAAACTTCTTTTTCAAGAAACTA
  AGAGGCAAGTATCACTTCAAATTGGAACGTTGAGAATGTCCAATTATCTTCTCCCTTTCT
  AACANAATGTTCTTTTAATAATTATGTTTAAGGCANAGAAACTAGCCTCTATTTTTCCAT
  ATTTCAGGNNATCTATTNNGAACTAGATACTTGCCAGTTCATTATTTGTGTATTAAACAC
  TGGATATATATTTCATATTAATATTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003762
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003762.2, NP_003753.2
• RefSeq Size: 1254 bp
• RefSeq ORF: 426 bp
• Locus ID: 8674
• UniProt ID: O75379
• Cytogenetics: 1q24.3
• Domains: synaptobrevin
• Protein Families: Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.