PRAF1 (POLR1E) (NM_022490) Human Untagged Clone

CAT#: SC111400

POLR1E (untagged)-Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRAF1
• Synonyms: A49; PAF53; PRAF1; RPA49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022490, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGAGGTGTTGCCGAGTGCGAGGTGGCAGTATTGTGGGGCGCCCGACGGGAGCCAGAGAGCTG
  TACTGGTCCAGTTCTCCAACGGGAAGCTACAGAGTCCAGGCAACATGCGCTTTACCTTGTATGAGAACAA
  AGATTCCACCAACCCCAGGAAGAGGAATCAACGGATCCTGGCAGCTGAAACAGATAGGCTCTCCTATGTG
  GGAAACAATTTTGGGACTGGAGCCCTCAAATGCAACACTTTGTGCAGGCACTTTGTGGGAATTTTGAACA
  AGACCTCTGGCCAAATGGAAGTATATGATGCTGAATTGTTCAACATGCAGCCACTATTTTCAGATGTATC
  AGTTGAGAGTGAACTGGCGCTAGAGAGTCAGACCAAAACTTACAGAGAAAAGATGGATTCTTGTATTGAA
  GCCTTTGGTACCACCAAACAGAAGCGAGCTCTGAACACCAGGAGAATGAACAGAGTTGGCAATGAATCTT
  TGAATCGTGCAGTGGCTAAAGCTGCAGAGACTATCATTGATACGAAGGGTGTGACTGCTCTGGTCAGCGA
  TGCTATCCACAATGACTTGCAAGATGACTCCCTCTACCTTCCTCCCTGCTATGATGATGCAGCCAAGCCT
  GAAGACGTGTATAAATTTGAAGATCTTCTTTCCCCTGCGGAGTATGAAGCTCTTCAGAGCCCATCTGAAG
  CTTTCAGGAACGTCACGTCAGAAGAAATACTGAAGATGATTGAGGAGAACAGCCATTGCACCTTTGTCAT
  AGAAGCGTTGAAGTCTTTGCCATCAGATGTGGAGAGCCGAGACCGCCAGGCCCGATGCATATGGTTTCTG
  GATACCCTCATCAAATTTCGAGCTCATAGGGTAGTTAAGCGGAAAAGTGCTCTGGGACCTGGAGTTCCCC
  ACATCATCAACACCAAACTGCTGAAGCACTTTACTTGCTTGACCTACAACAATGGCAGATTACGGAACTT
  AATTTCGGATTCTATGAAGGCGAAGATTACTGCATATGTGATCATACTTGCCTTGCACATACATGACTTC
  CAAATTGACCTGACAGTGTTACAGAGGGACTTGAAGCTCAGTGAGAAAAGGATGATGGAGATAGCCAAAG
  CCATGAGGCTGAAGATCTCCAAAAGAAGGGTGTCTGTGGCCGCCGGCAGTGAAGAAGATCACAAACTGGG
  CACCCTGTCCCTCCCGCTGCCTCCAGCCCAGACCTCAGACCGCCTGGCAAAGCGGAGGAAGATTACCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022490 unedited
  GCCGTTCGGGATTTGTATACGATTTACTATAGGCGGCCGCGCAATTCGCACGAGGCTCGG
  CCTGGGCTCCCGCGTGTTTAAAATGCGCTTGTGGCTGCTGCTGTCTTAACTCCTGTGCTT
  GGCGGACAGACAGGCGAGATGGCGGCGGAGGTGTTGCCGAGTGCGAGGTGGCAGTATTGT
  GGGGCGCCCGACGGGAGCCACAGAGCTACTCTGGTCCAGTTCTCCAACGGGAAGCTACAG
  AGTCCAGGCAACATGCGCTTTACCTTGTATGAGAACAAAGATTCCACCAACCCCAGGAAG
  AGGAATCAACGGATCCTGGCAGCTGAAACAGATAGGCTCTCCTATGTGGGAAACAATTTT
  GGGACTGGAGCCCTCAAATGCAACACTTTGTGCAGGCACTTTGTGGGAATTTTGAACAAG
  ACCTCTACCCAAATGGAAGTATATGATGCTGAATTGTTCAATATGCTGCCACTATTTTCA
  GATGTATCAGTTGAGAGTGAACTGGCGCTAGAGAGTCAGACCAAAACTTACAGAGAAAAG
  ATGGATTCTTGTATTGAAGCCTTTGGTACCACCAAACAGAAGCGAGCTCTGAACACCAGG
  AGAATGAACAGAGTTGGCAATGAATCTTTGAATCGTGCAGTGGCTAAAGCTGCAGAGACT
  ATCATTGATACGAAGGGTGTGACTGCTCTGGTCACCGATGCTTTCCCAATGACTTGCAAG
  ATGACTCCCTCTACCTTCCTNCCTGCTATGATGATGCACCCAAGCCTGAATACGTGTTTA
  AATTGTGAAATCTTTTTTTCCCTGCGGAGTATGAAACTCTTTCAAAACGCATCTGAAGCT
  TTCACGAACCTCCCGCCAAAAGAATACTGGACATAATGGGGAGAACAGCCTTTGCCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022490 unedited
  CCCTCACTTGCTANAGTCGAGAAATTCTTTTTTTTTTTTGAAGTCCAACCTTTCCATCGC
  TTTATATTTCTGCATAAGACAAGCACAGTTGAGATGCTGGCTTCTGGCTTCATACCTGTG
  CTAAAGCAAGGCTTCTTTTTTCCTTATGTTACTTTTTTGAGACAAGGTCTCACTCTGTGA
  CCCAGGATGGAGTGTGGCCACTCATCTTAGCTCACTGCAACCTTGATTTCCCAAGCTCAA
  GTAATCGTCCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCGCACACCACCAGGCCT
  GACTCTTTTTTTTTTCCTCCGGTAGAGATGGGGTCTCCCTATGTTGTCCAGACTGGTTTC
  AAACTCCTGAGCTCAATGATCTTCCTGCCTCGGCCTCCCAAAGTGCTGGGATTTCAGGTG
  TGAGCCACCATCCCCGGACCTTTTCTTTTCAAAACATACATAAAAATGGAAATGAATAGG
  ACCAGCCAGTGGCTGTGATGCAGCCAAAACGCCCTGTCTGGAAAGCATGCGTCTAGGTAA
  TCTTCCTCCGCTTCGCCAGGCGGTCTGACGTCTGGGCTGGAGGCACCGGGAGGGACAGGG
  TGCCCAGTTTGTGATCTTCTCTCACTGCCGGCGGCCACAGACACCCTTCTTTTGGAGATC
  TTCAGCCTCATGGCTTTGGCTATCTCCATCATCCTTTTCTCACTGAGCTTCAAGTCCCTC
  TGTAACACTGCCAGGTCAATTTGGAAGTCATGCATGTGCCAGGCAAGGATGATCACATAT
  GCAGCGATTCTCCGCCTTCATAGACACCCGAAATTAAGTTCCGTACTCTGCCATTGTTGT
  AAGGCAAGCACGCAAAGTGCTTCCACAGCTCCGGTGCTGCTGATGTGCGGAACTCCAGGC
  CCAGCACCCACTTTCCCTTATAAACCCTACGACCCGAACTTGCCGAGGGGTCCACGAACC
  TTGCATCGGCGCTGCGGCTTCGGCTCCCACATCGAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022490
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022490.1, NP_071935.1
• RefSeq Size: 1854 bp
• RefSeq ORF: 1260 bp
• Locus ID: 64425
• UniProt ID: Q9GZS1
• Cytogenetics: 9p13.2
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.