Proteasome subunit beta type 2 (PSMB2) (NM_002794) Human Untagged Clone

CAT#: SC110986

PSMB2 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Proteasome subunit beta type 2
• Synonyms: HC7-I
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110986 sequence for NM_002794 edited (data generated by NextGen Sequencing)
  ATGGAGTACCTCATCGGTATCCAAGGCCCCGACTATGTTCTTGTCGCCTCCGACCGGGTG
  GCCGCCAGCAATATTGTCCAGATGAAGGACGATCATGACAAGATGTTTAAGATGAGTGAA
  AAGATATTACTCCTGTGTGTTGGAGAGGCTGGAGACACTGTACAGTTTGCAGAATATATT
  CAGAAAAACGTGCAACTTTATAAGATGCGAAATGGATATGAATTGTCTCCCACGGCAGCA
  GCTAACTTCACACGCCGAAACCTGGCTGACTGTCTTCGGAGTCGGACCCCATATCATGTG
  AACCTCCTCCTGGCTGGCTATGATGAGCATGAAGGGCCAGCGCTGTATTACATGGACTAC
  CTGGCAGCCTTGGCCAAGGCCCCTTTTGCAGCCCACGGCTATGGTGCCTTCCTGACTCTC
  AGTATCCTCGACCGATACTACACACCGACTATCTCACGTGAGAGGGCAGTGGAACTCCTT
  AGGAAATGTCTGGAGGAGCTCCAGAAACGCTTCATCCTGAATCTGCCAACCTTCAGTGTT
  CGAATCATTGACAAAAATGGCATCCATGACCTGGATAACATTTCCTTCCCCAAACAGGGC
  TCCTAA
  
  Clone variation with respect to NM_002794.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002794 unedited
  TTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGCGCCAATCTTCGCTC
  TGAGGTGCTGTCTCACCGGTGAGACCTGGAAGCGGGCGAGTCTCGTGCTGTGTCGGACCT
  GCAGCCCCTGGCCTTCCGCCACCATGGAGTACCTCATCGGTATCCAAGGCCCCGACTATG
  TTCTTGTCGCCTCCGACCGGGTGGCCGCCAGCAATATTGTCCAGATGAAGGACGATCATG
  ACAAGATGTTTAAGATGAGTGAAAAGATATTACTCCTGTGTGTTGGAGAGGCTGGAGACA
  CTGTACAGTTTGCAGAATATATTCAGAAAAACGTGCAACTTTATAAGATGCGAAATGGAT
  ATGAATTGTCTCCCACGGCAGCAGCTAACTTCACACGCCGAAACCTGGCTGACTGTCTTC
  GGAGTCGGACCCCATATCATGTGAACCTCCTCCTGGCTGGCTATGATGAGCATGAAGGGC
  CAGCGCTGTATTACATGGACTACCTGGCAGCCTTGGCCAAGGCCCCTTTTGCAGCCCACG
  GCTATGGTGCCTTCCTGACTCTCAGTATCCTCGACCGATACTACACACCGACTATCTCAC
  GTGAGAGGGCAGTGGAACTCCTTAGGAAATGTCTGGAGGAGCTCCAGAAACGCTTCATCC
  TGAATCTGCCAACCTTCAGTGTTCGAATCATTGACAAAAATGGCATCCATGACCTGGATA
  ACATTTCCTTCCCCAACAGGGCTCCTACATCATGTCCTCCCTTCCACTTGCCAGAGAACT
  NTTTTTTGATGGGCTCCTTTATTNTTTTCTACTCTTTTCANGCGCACTCTTGATAAATGG
  GTATTAGAAATAAGGTGACTATGGATATAANTGAAAAAAAAAAAAAAACTCACTCTAGAT
  TGCGGCCGCGTCATAGCTGTTCCTGAAAGATTCCGGTGGCATCCCTGTGACCCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002794 unedited
  CCCCCCTCCCCATCCATCTCTTACCCAATCTATTCCCGTGTTTTAGTCTAGCACCGCGGA
  CCGCTTTCTAGNGATCGGTTTTTTTTTTTTTTTTTTCAATTATATCCATAGTCACCTTTA
  TTCTGAATTAACCATTTATCAAGAGTGCGCCTGAAAAGGTAGAAAAAAATAAAGGAGCCC
  ATCAAAAAAAAGTTCCCTGGCAAGTGGGAGGGAGGACATGATGTTAGGAGCCCTGTTTGG
  GGAAGGAAATGTTATCCAGGTCATGGATGCCATTTTTGTCAATGATTCGAACACTGAAGG
  TTGGCACATTCAGGATGAAGCGTTTTTGGAGCTCCTCCAGACATTTCCTAAGGAGTTCCA
  CTGCCCTCTCACGTGAGATAGTCGGTGTGTAGTATCGGTCGAGGATACTGAGAGTCAGGA
  AGGCACCATAGCCGTGGGCTGCAAAAGGGGCCTTGGCCAAGGCTGCCAGGTAGTCCATGT
  AATACAGCGCTGGCCCTTCATGCTCATCATAGCCAGCCAGGAGGAGGCTCACATGATATG
  GGGCCCGATTCCGAAACAGTCAGCCAGGTTTCGGCGTGTGAAGTCAGCTGCTGCCGTGGG
  AGACAATTCATATCCCATTCCGCATCTTATAAAGTTGCACGCTTTTCCTGAATATATTCT
  GCCAACTGCACAGTGTCCTACAGCTTTTCAACAAACAGGAGTAATATTTTTTTCCCTTAT
  NCTTAAACATTCTTGCCATGCACCGNTCCTTTATCTGGAACAATAATGGTTGGCGGCACA
  CCCCGTTCGGGAGGCGACCAAGACCATACTTCGGCGCCTTTGGATCCCGTAGAGGTCACT
  CCATGGGGGCCGAAAGCCCCTGGCCCGTAAGTTCCTAACACACCACAAAACTCTTCCCGT
  CTCCAAGTTTACCCGGTGAGAAACCCCTTAAAACCGAAATATTGCCCTCTCTCCCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002794
• Insert Size: 920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002794.3, NP_002785.1
• RefSeq Size: 850 bp
• RefSeq ORF: 606 bp
• Locus ID: 5690
• UniProt ID: P49721
• Cytogenetics: 1p34.3
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.